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Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial-mesenchymal transition of gastric cancer

LncRNAs play critical roles in gastric cancer (GC). In this study, the expression of fourteen cancer related lncRNAs were investigated in paired tissues of 66 patients with GC, Realtime RT-PCR revealed that ZFAS1 was significantly upregulated. We then examined the expression of ZFAS1 in plasmas deri...

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Autores principales: Zhou, Hu, Wang, Fubing, Chen, Hao, Tan, Qian, Qiu, Shili, Chen, Shanshan, Jing, Wei, Yu, Mingxia, Liang, Chunzi, Ye, Shengwei, Tu, Jiancheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5076450/
https://www.ncbi.nlm.nih.gov/pubmed/27654478
http://dx.doi.org/10.18632/aging.101048
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author Zhou, Hu
Wang, Fubing
Chen, Hao
Tan, Qian
Qiu, Shili
Chen, Shanshan
Jing, Wei
Yu, Mingxia
Liang, Chunzi
Ye, Shengwei
Tu, Jiancheng
author_facet Zhou, Hu
Wang, Fubing
Chen, Hao
Tan, Qian
Qiu, Shili
Chen, Shanshan
Jing, Wei
Yu, Mingxia
Liang, Chunzi
Ye, Shengwei
Tu, Jiancheng
author_sort Zhou, Hu
collection PubMed
description LncRNAs play critical roles in gastric cancer (GC). In this study, the expression of fourteen cancer related lncRNAs were investigated in paired tissues of 66 patients with GC, Realtime RT-PCR revealed that ZFAS1 was significantly upregulated. We then examined the expression of ZFAS1 in plasmas derived from 77 GC patients before- and post-operations and 60 healthy individuals, and found that circulating ZFAS1 was also upregulated in GC patients and operation can reduce its presence in plasma. To investigate the potential mechanisms, we compared the expression of ZFAS1 in multiple gastric cell lines and one normal cell line and found that ZFAS1 was up-regulated in GC cell lines. Furthermore, circulating tumor cells (CTC) were simulated by mixing GC cells with peripheral blood. After EpCAM antibody-based cell sorting, we found that the expression of ZFAS1 was positively correlated with EMT property of CTCs. In GC patient tissue samples, we found that Twist was positively correlated with ZFAS1 by immunohistochemical staining. Taken together, our results suggested that ZFAS1 was up-regulated in both tissues and plasmas of GC patients, and may be involved in regulation of EMT in GC progression. Thus, ZFAS1 might serve as a potential diagnostic marker and/or therapeutic target for GC.
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spelling pubmed-50764502016-10-27 Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial-mesenchymal transition of gastric cancer Zhou, Hu Wang, Fubing Chen, Hao Tan, Qian Qiu, Shili Chen, Shanshan Jing, Wei Yu, Mingxia Liang, Chunzi Ye, Shengwei Tu, Jiancheng Aging (Albany NY) Research Paper LncRNAs play critical roles in gastric cancer (GC). In this study, the expression of fourteen cancer related lncRNAs were investigated in paired tissues of 66 patients with GC, Realtime RT-PCR revealed that ZFAS1 was significantly upregulated. We then examined the expression of ZFAS1 in plasmas derived from 77 GC patients before- and post-operations and 60 healthy individuals, and found that circulating ZFAS1 was also upregulated in GC patients and operation can reduce its presence in plasma. To investigate the potential mechanisms, we compared the expression of ZFAS1 in multiple gastric cell lines and one normal cell line and found that ZFAS1 was up-regulated in GC cell lines. Furthermore, circulating tumor cells (CTC) were simulated by mixing GC cells with peripheral blood. After EpCAM antibody-based cell sorting, we found that the expression of ZFAS1 was positively correlated with EMT property of CTCs. In GC patient tissue samples, we found that Twist was positively correlated with ZFAS1 by immunohistochemical staining. Taken together, our results suggested that ZFAS1 was up-regulated in both tissues and plasmas of GC patients, and may be involved in regulation of EMT in GC progression. Thus, ZFAS1 might serve as a potential diagnostic marker and/or therapeutic target for GC. Impact Journals LLC 2016-09-18 /pmc/articles/PMC5076450/ /pubmed/27654478 http://dx.doi.org/10.18632/aging.101048 Text en Copyright: © 2016 Zhou et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Zhou, Hu
Wang, Fubing
Chen, Hao
Tan, Qian
Qiu, Shili
Chen, Shanshan
Jing, Wei
Yu, Mingxia
Liang, Chunzi
Ye, Shengwei
Tu, Jiancheng
Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial-mesenchymal transition of gastric cancer
title Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial-mesenchymal transition of gastric cancer
title_full Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial-mesenchymal transition of gastric cancer
title_fullStr Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial-mesenchymal transition of gastric cancer
title_full_unstemmed Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial-mesenchymal transition of gastric cancer
title_short Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial-mesenchymal transition of gastric cancer
title_sort increased expression of long-noncoding rna zfas1 is associated with epithelial-mesenchymal transition of gastric cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5076450/
https://www.ncbi.nlm.nih.gov/pubmed/27654478
http://dx.doi.org/10.18632/aging.101048
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