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Evaluation of Biogenic Amines Levels, and Biochemical and Microbiological Characterization of Italian-type Salami Sold in Rio de Janeiro, Brazil

The objective of the present study was to evaluate the levels of biogenic amines (cadaverine, putrescine, tyramine, histamine, spermidine and spermine) by high performance liquid chromatography (HPLC) and the physicochemical (moisture, lipids, proteins, pH, water activity and fixed mineral residue)...

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Autores principales: dos Santos, Luiz Felipe Lopes, Mársico, Eliane Teixeira, Lázaro, César Aquiles, Teixeira, Rose, Doro, Laís, Júnior, Carlos Adam Conte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5076629/
https://www.ncbi.nlm.nih.gov/pubmed/27800400
http://dx.doi.org/10.4081/ijfs.2015.4048
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author dos Santos, Luiz Felipe Lopes
Mársico, Eliane Teixeira
Lázaro, César Aquiles
Teixeira, Rose
Doro, Laís
Júnior, Carlos Adam Conte
author_facet dos Santos, Luiz Felipe Lopes
Mársico, Eliane Teixeira
Lázaro, César Aquiles
Teixeira, Rose
Doro, Laís
Júnior, Carlos Adam Conte
author_sort dos Santos, Luiz Felipe Lopes
collection PubMed
description The objective of the present study was to evaluate the levels of biogenic amines (cadaverine, putrescine, tyramine, histamine, spermidine and spermine) by high performance liquid chromatography (HPLC) and the physicochemical (moisture, lipids, proteins, pH, water activity and fixed mineral residue) and microbiological (lactic acid bacteria and aerobic heterotrophic mesophilic bacteria count) characteristics of six Italian-type salami brands sold in the city of Niteroi (Rio de Janeiro, Brazil). The salami showed lactic acid bacteria count from 5.7 to 8.6 CFU•mL(-1), and heterotrophic mesophilic bacteria count from 5.8 to 8.7 CFU•mL(-1). Three brands showed moisture contents above 35% and one brand had protein content below 25%. The mean values obtained for the amines were: 197.43, 143.29, 73.02, 4.52, 90.66 and 36.17 mg•kg(-1) for tyramine, putrescine, cadaverine, spermidine, histamine, and spermine respectively. Two brands presented histamine contents above the legal limit established in 100 mg•kg(-1). We concluded that the evaluated salami presented a wide variation in the count of the bacterial groups with a predominance of lactic acid bacteria. The moisture contents indicate insufficient drying before commercialization and protein content had values below the minimum limit determined by the Brazilian legislation. Finally, the levels of biogenic amines found could cause adverse reactions in susceptible consumers, depending of the amount and frequency of intake of these products.
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spelling pubmed-50766292016-10-31 Evaluation of Biogenic Amines Levels, and Biochemical and Microbiological Characterization of Italian-type Salami Sold in Rio de Janeiro, Brazil dos Santos, Luiz Felipe Lopes Mársico, Eliane Teixeira Lázaro, César Aquiles Teixeira, Rose Doro, Laís Júnior, Carlos Adam Conte Ital J Food Saf Article The objective of the present study was to evaluate the levels of biogenic amines (cadaverine, putrescine, tyramine, histamine, spermidine and spermine) by high performance liquid chromatography (HPLC) and the physicochemical (moisture, lipids, proteins, pH, water activity and fixed mineral residue) and microbiological (lactic acid bacteria and aerobic heterotrophic mesophilic bacteria count) characteristics of six Italian-type salami brands sold in the city of Niteroi (Rio de Janeiro, Brazil). The salami showed lactic acid bacteria count from 5.7 to 8.6 CFU•mL(-1), and heterotrophic mesophilic bacteria count from 5.8 to 8.7 CFU•mL(-1). Three brands showed moisture contents above 35% and one brand had protein content below 25%. The mean values obtained for the amines were: 197.43, 143.29, 73.02, 4.52, 90.66 and 36.17 mg•kg(-1) for tyramine, putrescine, cadaverine, spermidine, histamine, and spermine respectively. Two brands presented histamine contents above the legal limit established in 100 mg•kg(-1). We concluded that the evaluated salami presented a wide variation in the count of the bacterial groups with a predominance of lactic acid bacteria. The moisture contents indicate insufficient drying before commercialization and protein content had values below the minimum limit determined by the Brazilian legislation. Finally, the levels of biogenic amines found could cause adverse reactions in susceptible consumers, depending of the amount and frequency of intake of these products. PAGEPress Publications, Pavia, Italy 2015-08-28 /pmc/articles/PMC5076629/ /pubmed/27800400 http://dx.doi.org/10.4081/ijfs.2015.4048 Text en ©Copyright L.F. Lopes dos Santos et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution 3.0 License (by-nc 3.0). (http://creativecommons.org/licenses/by-nc/3.0/)
spellingShingle Article
dos Santos, Luiz Felipe Lopes
Mársico, Eliane Teixeira
Lázaro, César Aquiles
Teixeira, Rose
Doro, Laís
Júnior, Carlos Adam Conte
Evaluation of Biogenic Amines Levels, and Biochemical and Microbiological Characterization of Italian-type Salami Sold in Rio de Janeiro, Brazil
title Evaluation of Biogenic Amines Levels, and Biochemical and Microbiological Characterization of Italian-type Salami Sold in Rio de Janeiro, Brazil
title_full Evaluation of Biogenic Amines Levels, and Biochemical and Microbiological Characterization of Italian-type Salami Sold in Rio de Janeiro, Brazil
title_fullStr Evaluation of Biogenic Amines Levels, and Biochemical and Microbiological Characterization of Italian-type Salami Sold in Rio de Janeiro, Brazil
title_full_unstemmed Evaluation of Biogenic Amines Levels, and Biochemical and Microbiological Characterization of Italian-type Salami Sold in Rio de Janeiro, Brazil
title_short Evaluation of Biogenic Amines Levels, and Biochemical and Microbiological Characterization of Italian-type Salami Sold in Rio de Janeiro, Brazil
title_sort evaluation of biogenic amines levels, and biochemical and microbiological characterization of italian-type salami sold in rio de janeiro, brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5076629/
https://www.ncbi.nlm.nih.gov/pubmed/27800400
http://dx.doi.org/10.4081/ijfs.2015.4048
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