Cargando…

Galectin-1 is essential for efficient liver regeneration following hepatectomy

Galectin-1 (Gal1) is a known immune/inflammatory regulator which acts both extracellularly and intracellularly, modulating innate and adaptive immune responses. Here, we explored the role of Gal1 in liver regeneration using 70% partial hepatectomy (PHx) of C57BL/6 wild type and Gal1-knockout (Gal1-K...

Descripción completa

Detalles Bibliográficos
Autores principales: Potikha, Tamara, Ella, Ezra, Cerliani, Juan P., Mizrahi, Lina, Pappo, Orit, Rabinovich, Gabriel A., Galun, Eithan, Goldenberg, Daniel S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5077973/
https://www.ncbi.nlm.nih.gov/pubmed/27166189
http://dx.doi.org/10.18632/oncotarget.9194
_version_ 1782462279206305792
author Potikha, Tamara
Ella, Ezra
Cerliani, Juan P.
Mizrahi, Lina
Pappo, Orit
Rabinovich, Gabriel A.
Galun, Eithan
Goldenberg, Daniel S.
author_facet Potikha, Tamara
Ella, Ezra
Cerliani, Juan P.
Mizrahi, Lina
Pappo, Orit
Rabinovich, Gabriel A.
Galun, Eithan
Goldenberg, Daniel S.
author_sort Potikha, Tamara
collection PubMed
description Galectin-1 (Gal1) is a known immune/inflammatory regulator which acts both extracellularly and intracellularly, modulating innate and adaptive immune responses. Here, we explored the role of Gal1 in liver regeneration using 70% partial hepatectomy (PHx) of C57BL/6 wild type and Gal1-knockout (Gal1-KO, Lgals1−/−) mice. Gene or protein expression, in liver samples collected at time intervals from 2 to 168 hours post-operation, was tested by either RT-PCR or by immunoblotting and immunohistochemistry, respectively. We demonstrated that Gal1 transcript and protein expression was induced in the liver tissue of wild type mice upon PHx. Liver regeneration following PHx was significantly delayed in the Gal1-KO compared to the control liver. This delay was accompanied by a decreased Akt phosphorylation, and accumulation of the hepatocyte nuclear p21 protein in the Gal1-KO versus control livers at 24 and 48 hours following PHx. Transcripts of several known regulators of inflammation, cell cycle and cell signaling, including some known PHx-induced genes, were aberrantly expressed (mainly down-regulated) in Gal1-KO compared to control livers at 2, 6 and 24 hours post-PHx. Transient steatosis, which is imperative for liver regeneration following PHx, was significantly delayed and decreased in the Gal1-KO compared to the control liver and was accompanied by a significantly decreased expression in the mutant liver of several genes encoding lipid metabolism regulators. Our results demonstrate that Gal1 protein is essential for efficient liver regeneration following PHx through the regulation of liver inflammation, hepatic cell proliferation, and the control of lipid storage in the regenerating liver.
format Online
Article
Text
id pubmed-5077973
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-50779732016-10-28 Galectin-1 is essential for efficient liver regeneration following hepatectomy Potikha, Tamara Ella, Ezra Cerliani, Juan P. Mizrahi, Lina Pappo, Orit Rabinovich, Gabriel A. Galun, Eithan Goldenberg, Daniel S. Oncotarget Research Paper: Pathology Galectin-1 (Gal1) is a known immune/inflammatory regulator which acts both extracellularly and intracellularly, modulating innate and adaptive immune responses. Here, we explored the role of Gal1 in liver regeneration using 70% partial hepatectomy (PHx) of C57BL/6 wild type and Gal1-knockout (Gal1-KO, Lgals1−/−) mice. Gene or protein expression, in liver samples collected at time intervals from 2 to 168 hours post-operation, was tested by either RT-PCR or by immunoblotting and immunohistochemistry, respectively. We demonstrated that Gal1 transcript and protein expression was induced in the liver tissue of wild type mice upon PHx. Liver regeneration following PHx was significantly delayed in the Gal1-KO compared to the control liver. This delay was accompanied by a decreased Akt phosphorylation, and accumulation of the hepatocyte nuclear p21 protein in the Gal1-KO versus control livers at 24 and 48 hours following PHx. Transcripts of several known regulators of inflammation, cell cycle and cell signaling, including some known PHx-induced genes, were aberrantly expressed (mainly down-regulated) in Gal1-KO compared to control livers at 2, 6 and 24 hours post-PHx. Transient steatosis, which is imperative for liver regeneration following PHx, was significantly delayed and decreased in the Gal1-KO compared to the control liver and was accompanied by a significantly decreased expression in the mutant liver of several genes encoding lipid metabolism regulators. Our results demonstrate that Gal1 protein is essential for efficient liver regeneration following PHx through the regulation of liver inflammation, hepatic cell proliferation, and the control of lipid storage in the regenerating liver. Impact Journals LLC 2016-05-05 /pmc/articles/PMC5077973/ /pubmed/27166189 http://dx.doi.org/10.18632/oncotarget.9194 Text en Copyright: © 2016 Potikha et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper: Pathology
Potikha, Tamara
Ella, Ezra
Cerliani, Juan P.
Mizrahi, Lina
Pappo, Orit
Rabinovich, Gabriel A.
Galun, Eithan
Goldenberg, Daniel S.
Galectin-1 is essential for efficient liver regeneration following hepatectomy
title Galectin-1 is essential for efficient liver regeneration following hepatectomy
title_full Galectin-1 is essential for efficient liver regeneration following hepatectomy
title_fullStr Galectin-1 is essential for efficient liver regeneration following hepatectomy
title_full_unstemmed Galectin-1 is essential for efficient liver regeneration following hepatectomy
title_short Galectin-1 is essential for efficient liver regeneration following hepatectomy
title_sort galectin-1 is essential for efficient liver regeneration following hepatectomy
topic Research Paper: Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5077973/
https://www.ncbi.nlm.nih.gov/pubmed/27166189
http://dx.doi.org/10.18632/oncotarget.9194
work_keys_str_mv AT potikhatamara galectin1isessentialforefficientliverregenerationfollowinghepatectomy
AT ellaezra galectin1isessentialforefficientliverregenerationfollowinghepatectomy
AT cerlianijuanp galectin1isessentialforefficientliverregenerationfollowinghepatectomy
AT mizrahilina galectin1isessentialforefficientliverregenerationfollowinghepatectomy
AT pappoorit galectin1isessentialforefficientliverregenerationfollowinghepatectomy
AT rabinovichgabriela galectin1isessentialforefficientliverregenerationfollowinghepatectomy
AT galuneithan galectin1isessentialforefficientliverregenerationfollowinghepatectomy
AT goldenbergdaniels galectin1isessentialforefficientliverregenerationfollowinghepatectomy