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COMMD7 is correlated with a novel NF-κB positive feedback loop in hepatocellular carcinoma

The correlation between nuclear factor-kappa B (NF-κB) and COMMD7 in hepatocellular carcinoma (HCC) development remained unclear. Here, our clinicopathological data showed that COMMD7 is overexpressed in HCC with a correlation to NF-κB. Using HepG2 and SMMC-7721 cells that aberrantly overexpressed C...

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Autores principales: Zheng, Lu, Deng, Chang-Lin, Wang, Liang, Huang, Xiao-Bing, You, Nan, Tang, Yi-Chen, Wu, Ke, Liang, Ping, Mi, Na, Li, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5078050/
https://www.ncbi.nlm.nih.gov/pubmed/27129158
http://dx.doi.org/10.18632/oncotarget.9047
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author Zheng, Lu
Deng, Chang-Lin
Wang, Liang
Huang, Xiao-Bing
You, Nan
Tang, Yi-Chen
Wu, Ke
Liang, Ping
Mi, Na
Li, Jing
author_facet Zheng, Lu
Deng, Chang-Lin
Wang, Liang
Huang, Xiao-Bing
You, Nan
Tang, Yi-Chen
Wu, Ke
Liang, Ping
Mi, Na
Li, Jing
author_sort Zheng, Lu
collection PubMed
description The correlation between nuclear factor-kappa B (NF-κB) and COMMD7 in hepatocellular carcinoma (HCC) development remained unclear. Here, our clinicopathological data showed that COMMD7 is overexpressed in HCC with a correlation to NF-κB. Using HepG2 and SMMC-7721 cells that aberrantly overexpressed COMMD7, we found that NF-κB directly binds with COMMD7 promoter and serves as an activator for COMMD7 transcription by luciferase reporter assay, chromatin immunoprecipitation (ChIP), and electrophoretic mobility shift assay (EMSA). In both HepG2 cells and SMMC-7721 cells, the silencing of COMMD7 significantly inhibited the cell proliferation, whereas NF-κB silencing inhibited the expression of COMMD7 and further inhibited cell proliferation. In addition, cell apoptosis was promoted by COMMD7 silencing, and further promoted by NF-κB silencing. Cell migration and invasion were also inhibited by COMMD7 silencing, and further inhibited by NF-κB silencing. Thus, COMMD7 is correlated with a novel NF-κB positive feedback loop in hepatocellular carcinoma. Developing strategies for the treatment of HCC should consider the correlation between NF-κB and COMMD7, so as to improve the specificity and sensitivity of therapy and to reduce toxicity.
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spelling pubmed-50780502016-10-28 COMMD7 is correlated with a novel NF-κB positive feedback loop in hepatocellular carcinoma Zheng, Lu Deng, Chang-Lin Wang, Liang Huang, Xiao-Bing You, Nan Tang, Yi-Chen Wu, Ke Liang, Ping Mi, Na Li, Jing Oncotarget Research Paper The correlation between nuclear factor-kappa B (NF-κB) and COMMD7 in hepatocellular carcinoma (HCC) development remained unclear. Here, our clinicopathological data showed that COMMD7 is overexpressed in HCC with a correlation to NF-κB. Using HepG2 and SMMC-7721 cells that aberrantly overexpressed COMMD7, we found that NF-κB directly binds with COMMD7 promoter and serves as an activator for COMMD7 transcription by luciferase reporter assay, chromatin immunoprecipitation (ChIP), and electrophoretic mobility shift assay (EMSA). In both HepG2 cells and SMMC-7721 cells, the silencing of COMMD7 significantly inhibited the cell proliferation, whereas NF-κB silencing inhibited the expression of COMMD7 and further inhibited cell proliferation. In addition, cell apoptosis was promoted by COMMD7 silencing, and further promoted by NF-κB silencing. Cell migration and invasion were also inhibited by COMMD7 silencing, and further inhibited by NF-κB silencing. Thus, COMMD7 is correlated with a novel NF-κB positive feedback loop in hepatocellular carcinoma. Developing strategies for the treatment of HCC should consider the correlation between NF-κB and COMMD7, so as to improve the specificity and sensitivity of therapy and to reduce toxicity. Impact Journals LLC 2016-04-27 /pmc/articles/PMC5078050/ /pubmed/27129158 http://dx.doi.org/10.18632/oncotarget.9047 Text en Copyright: © 2016 Zheng et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Zheng, Lu
Deng, Chang-Lin
Wang, Liang
Huang, Xiao-Bing
You, Nan
Tang, Yi-Chen
Wu, Ke
Liang, Ping
Mi, Na
Li, Jing
COMMD7 is correlated with a novel NF-κB positive feedback loop in hepatocellular carcinoma
title COMMD7 is correlated with a novel NF-κB positive feedback loop in hepatocellular carcinoma
title_full COMMD7 is correlated with a novel NF-κB positive feedback loop in hepatocellular carcinoma
title_fullStr COMMD7 is correlated with a novel NF-κB positive feedback loop in hepatocellular carcinoma
title_full_unstemmed COMMD7 is correlated with a novel NF-κB positive feedback loop in hepatocellular carcinoma
title_short COMMD7 is correlated with a novel NF-κB positive feedback loop in hepatocellular carcinoma
title_sort commd7 is correlated with a novel nf-κb positive feedback loop in hepatocellular carcinoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5078050/
https://www.ncbi.nlm.nih.gov/pubmed/27129158
http://dx.doi.org/10.18632/oncotarget.9047
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