Cargando…

Characterization of Innate Immune Responses of Human Endothelial Cells Induced by Porphyromonas gingivalis and Their Derived Outer Membrane Vesicles

Atherosclerosis, a chronic inflammatory disease of the blood vessels, is one of the most common causes of morbidity and mortality world-wide. Involvement of Porphyromonas gingivalis in atherosclerosis is supported by observations from epidemiological, clinical, immunological, and molecular studies....

Descripción completa

Detalles Bibliográficos
Autores principales: Ho, Meng-Hsuan, Guo, Zhong-Mao, Chunga, Julio, Goodwin, J. Shawn, Xie, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5078693/
https://www.ncbi.nlm.nih.gov/pubmed/27826542
http://dx.doi.org/10.3389/fcimb.2016.00139
_version_ 1782462433735999488
author Ho, Meng-Hsuan
Guo, Zhong-Mao
Chunga, Julio
Goodwin, J. Shawn
Xie, Hua
author_facet Ho, Meng-Hsuan
Guo, Zhong-Mao
Chunga, Julio
Goodwin, J. Shawn
Xie, Hua
author_sort Ho, Meng-Hsuan
collection PubMed
description Atherosclerosis, a chronic inflammatory disease of the blood vessels, is one of the most common causes of morbidity and mortality world-wide. Involvement of Porphyromonas gingivalis in atherosclerosis is supported by observations from epidemiological, clinical, immunological, and molecular studies. Previously we reported that P. gingivalis vesicles have a much higher invasive efficiency than their originating cells. Here, we further compare the role of P. gingivalis cells and their vesicles in expression of chemoattractant proteins including CXCL1, CXCL2, and CXCL8, and adhesive molecules such as E-selectin in human umbilical vein endothelial cells (HUVECs). Both P. gingivalis 33277 cells and vesicles were able to up-regulate expression of these molecules, while the vesicles acted as more potent inducers of the inflammatory response associated with the development of atherosclerosis, consequently resulting in significant monocyte adhesion to a monolayer of HUVECs. Interestingly, we found that elevated expression of CXCL8 and E-selectin in endothelial cells induced by P. gingivalis correlated with the invasive ability of P. gingivalis cells and vesicles. Non-invasive bacterial cells and vesicles had no effect on expression of these genes. This study highlights the potential risk of P. gingivalis cells and vesicles in initiation of atherosclerosis and provides a potential target for the development of novel therapeutics against bacteria-associated atherosclerosis.
format Online
Article
Text
id pubmed-5078693
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-50786932016-11-08 Characterization of Innate Immune Responses of Human Endothelial Cells Induced by Porphyromonas gingivalis and Their Derived Outer Membrane Vesicles Ho, Meng-Hsuan Guo, Zhong-Mao Chunga, Julio Goodwin, J. Shawn Xie, Hua Front Cell Infect Microbiol Microbiology Atherosclerosis, a chronic inflammatory disease of the blood vessels, is one of the most common causes of morbidity and mortality world-wide. Involvement of Porphyromonas gingivalis in atherosclerosis is supported by observations from epidemiological, clinical, immunological, and molecular studies. Previously we reported that P. gingivalis vesicles have a much higher invasive efficiency than their originating cells. Here, we further compare the role of P. gingivalis cells and their vesicles in expression of chemoattractant proteins including CXCL1, CXCL2, and CXCL8, and adhesive molecules such as E-selectin in human umbilical vein endothelial cells (HUVECs). Both P. gingivalis 33277 cells and vesicles were able to up-regulate expression of these molecules, while the vesicles acted as more potent inducers of the inflammatory response associated with the development of atherosclerosis, consequently resulting in significant monocyte adhesion to a monolayer of HUVECs. Interestingly, we found that elevated expression of CXCL8 and E-selectin in endothelial cells induced by P. gingivalis correlated with the invasive ability of P. gingivalis cells and vesicles. Non-invasive bacterial cells and vesicles had no effect on expression of these genes. This study highlights the potential risk of P. gingivalis cells and vesicles in initiation of atherosclerosis and provides a potential target for the development of novel therapeutics against bacteria-associated atherosclerosis. Frontiers Media S.A. 2016-10-25 /pmc/articles/PMC5078693/ /pubmed/27826542 http://dx.doi.org/10.3389/fcimb.2016.00139 Text en Copyright © 2016 Ho, Guo, Chunga, Goodwin and Xie. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ho, Meng-Hsuan
Guo, Zhong-Mao
Chunga, Julio
Goodwin, J. Shawn
Xie, Hua
Characterization of Innate Immune Responses of Human Endothelial Cells Induced by Porphyromonas gingivalis and Their Derived Outer Membrane Vesicles
title Characterization of Innate Immune Responses of Human Endothelial Cells Induced by Porphyromonas gingivalis and Their Derived Outer Membrane Vesicles
title_full Characterization of Innate Immune Responses of Human Endothelial Cells Induced by Porphyromonas gingivalis and Their Derived Outer Membrane Vesicles
title_fullStr Characterization of Innate Immune Responses of Human Endothelial Cells Induced by Porphyromonas gingivalis and Their Derived Outer Membrane Vesicles
title_full_unstemmed Characterization of Innate Immune Responses of Human Endothelial Cells Induced by Porphyromonas gingivalis and Their Derived Outer Membrane Vesicles
title_short Characterization of Innate Immune Responses of Human Endothelial Cells Induced by Porphyromonas gingivalis and Their Derived Outer Membrane Vesicles
title_sort characterization of innate immune responses of human endothelial cells induced by porphyromonas gingivalis and their derived outer membrane vesicles
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5078693/
https://www.ncbi.nlm.nih.gov/pubmed/27826542
http://dx.doi.org/10.3389/fcimb.2016.00139
work_keys_str_mv AT homenghsuan characterizationofinnateimmuneresponsesofhumanendothelialcellsinducedbyporphyromonasgingivalisandtheirderivedoutermembranevesicles
AT guozhongmao characterizationofinnateimmuneresponsesofhumanendothelialcellsinducedbyporphyromonasgingivalisandtheirderivedoutermembranevesicles
AT chungajulio characterizationofinnateimmuneresponsesofhumanendothelialcellsinducedbyporphyromonasgingivalisandtheirderivedoutermembranevesicles
AT goodwinjshawn characterizationofinnateimmuneresponsesofhumanendothelialcellsinducedbyporphyromonasgingivalisandtheirderivedoutermembranevesicles
AT xiehua characterizationofinnateimmuneresponsesofhumanendothelialcellsinducedbyporphyromonasgingivalisandtheirderivedoutermembranevesicles