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Microparticles in the blood of patients with systemic lupus erythematosus (SLE): phenotypic characterization and clinical associations
Systemic lupus erythematosus (SLE) is a prototypic autoimmune disease characterized by circulating autoantibodies and the formation of immune complexes. In these responses, the selecting self-antigens likely derive from the remains of dead and dying cells, as well as from disturbances in clearance....
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5078765/ https://www.ncbi.nlm.nih.gov/pubmed/27777414 http://dx.doi.org/10.1038/srep36025 |
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author | Mobarrez, Fariborz Vikerfors, Anna Gustafsson, Johanna T. Gunnarsson, Iva Zickert, Agneta Larsson, Anders Pisetsky, David S. Wallén, Håkan Svenungsson, Elisabet |
author_facet | Mobarrez, Fariborz Vikerfors, Anna Gustafsson, Johanna T. Gunnarsson, Iva Zickert, Agneta Larsson, Anders Pisetsky, David S. Wallén, Håkan Svenungsson, Elisabet |
author_sort | Mobarrez, Fariborz |
collection | PubMed |
description | Systemic lupus erythematosus (SLE) is a prototypic autoimmune disease characterized by circulating autoantibodies and the formation of immune complexes. In these responses, the selecting self-antigens likely derive from the remains of dead and dying cells, as well as from disturbances in clearance. During cell death/activation, microparticles (MPs) can be released to the circulation. Previous MP studies in SLE have been limited in size and differ regarding numbers and phenotypes. Therefore, to characterize MPs more completely, we investigated 280 SLE patients and 280 individually matched controls. MPs were measured with flow cytometry and phenotyped according to phosphatidylserine expression (PS(+)/PS(−)), cellular origin and inflammatory markers. MPs, regardless of phenotype, are 2–10 times more abundant in SLE blood compared to controls. PS(−) MPs predominated in SLE, but not in controls (66% vs. 42%). Selectively in SLE, PS(−) MPs were more numerous in females and smokers. MP numbers decreased with declining renal function, but no clear association with disease activity was observed. The striking abundance of MPs, especially PS(−) MPs, suggests a generalized disturbance in SLE. MPs may be regarded as “liquid biopsies” to assess the production and clearance of dead, dying and activated cells, i.e. pivotal events for SLE pathogenesis. |
format | Online Article Text |
id | pubmed-5078765 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50787652016-10-28 Microparticles in the blood of patients with systemic lupus erythematosus (SLE): phenotypic characterization and clinical associations Mobarrez, Fariborz Vikerfors, Anna Gustafsson, Johanna T. Gunnarsson, Iva Zickert, Agneta Larsson, Anders Pisetsky, David S. Wallén, Håkan Svenungsson, Elisabet Sci Rep Article Systemic lupus erythematosus (SLE) is a prototypic autoimmune disease characterized by circulating autoantibodies and the formation of immune complexes. In these responses, the selecting self-antigens likely derive from the remains of dead and dying cells, as well as from disturbances in clearance. During cell death/activation, microparticles (MPs) can be released to the circulation. Previous MP studies in SLE have been limited in size and differ regarding numbers and phenotypes. Therefore, to characterize MPs more completely, we investigated 280 SLE patients and 280 individually matched controls. MPs were measured with flow cytometry and phenotyped according to phosphatidylserine expression (PS(+)/PS(−)), cellular origin and inflammatory markers. MPs, regardless of phenotype, are 2–10 times more abundant in SLE blood compared to controls. PS(−) MPs predominated in SLE, but not in controls (66% vs. 42%). Selectively in SLE, PS(−) MPs were more numerous in females and smokers. MP numbers decreased with declining renal function, but no clear association with disease activity was observed. The striking abundance of MPs, especially PS(−) MPs, suggests a generalized disturbance in SLE. MPs may be regarded as “liquid biopsies” to assess the production and clearance of dead, dying and activated cells, i.e. pivotal events for SLE pathogenesis. Nature Publishing Group 2016-10-25 /pmc/articles/PMC5078765/ /pubmed/27777414 http://dx.doi.org/10.1038/srep36025 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Mobarrez, Fariborz Vikerfors, Anna Gustafsson, Johanna T. Gunnarsson, Iva Zickert, Agneta Larsson, Anders Pisetsky, David S. Wallén, Håkan Svenungsson, Elisabet Microparticles in the blood of patients with systemic lupus erythematosus (SLE): phenotypic characterization and clinical associations |
title | Microparticles in the blood of patients with systemic lupus erythematosus (SLE): phenotypic characterization and clinical associations |
title_full | Microparticles in the blood of patients with systemic lupus erythematosus (SLE): phenotypic characterization and clinical associations |
title_fullStr | Microparticles in the blood of patients with systemic lupus erythematosus (SLE): phenotypic characterization and clinical associations |
title_full_unstemmed | Microparticles in the blood of patients with systemic lupus erythematosus (SLE): phenotypic characterization and clinical associations |
title_short | Microparticles in the blood of patients with systemic lupus erythematosus (SLE): phenotypic characterization and clinical associations |
title_sort | microparticles in the blood of patients with systemic lupus erythematosus (sle): phenotypic characterization and clinical associations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5078765/ https://www.ncbi.nlm.nih.gov/pubmed/27777414 http://dx.doi.org/10.1038/srep36025 |
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