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Chromatin proteins and RNA are associated with DNA during all phases of mitosis
Mitosis brings about major changes to chromosome and nuclear structure. We used recently developed proximity ligation assay-based techniques to investigate the association with DNA of chromatin-associated proteins and RNAs in Drosophila embryos during mitosis. All groups of tested proteins, histone-...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5078801/ https://www.ncbi.nlm.nih.gov/pubmed/27807477 http://dx.doi.org/10.1038/celldisc.2016.38 |
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author | L Black, Kathryn Petruk, Svetlana Fenstermaker, Tyler K Hodgson, Jacob W Caplan, Jeffrey L Brock, Hugh W Mazo, Alexander |
author_facet | L Black, Kathryn Petruk, Svetlana Fenstermaker, Tyler K Hodgson, Jacob W Caplan, Jeffrey L Brock, Hugh W Mazo, Alexander |
author_sort | L Black, Kathryn |
collection | PubMed |
description | Mitosis brings about major changes to chromosome and nuclear structure. We used recently developed proximity ligation assay-based techniques to investigate the association with DNA of chromatin-associated proteins and RNAs in Drosophila embryos during mitosis. All groups of tested proteins, histone-modifying and chromatin-remodeling proteins and methylated histones remained in close proximity to DNA during all phases of mitosis. We also found that RNA transcripts are associated with DNA during all stages of mitosis. Reduction of H3K27me3 levels or elimination of RNAs had no effect on the association of the components of PcG and TrxG complexes to DNA. Using a combination of proximity ligation assay-based techniques and super-resolution microscopy, we found that the number of protein–DNA and RNA–DNA foci undergoes significant reduction during mitosis, suggesting that mitosis may be accompanied by structural re-arrangement or compaction of specific chromatin domains. |
format | Online Article Text |
id | pubmed-5078801 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50788012016-11-02 Chromatin proteins and RNA are associated with DNA during all phases of mitosis L Black, Kathryn Petruk, Svetlana Fenstermaker, Tyler K Hodgson, Jacob W Caplan, Jeffrey L Brock, Hugh W Mazo, Alexander Cell Discov Article Mitosis brings about major changes to chromosome and nuclear structure. We used recently developed proximity ligation assay-based techniques to investigate the association with DNA of chromatin-associated proteins and RNAs in Drosophila embryos during mitosis. All groups of tested proteins, histone-modifying and chromatin-remodeling proteins and methylated histones remained in close proximity to DNA during all phases of mitosis. We also found that RNA transcripts are associated with DNA during all stages of mitosis. Reduction of H3K27me3 levels or elimination of RNAs had no effect on the association of the components of PcG and TrxG complexes to DNA. Using a combination of proximity ligation assay-based techniques and super-resolution microscopy, we found that the number of protein–DNA and RNA–DNA foci undergoes significant reduction during mitosis, suggesting that mitosis may be accompanied by structural re-arrangement or compaction of specific chromatin domains. Nature Publishing Group 2016-10-25 /pmc/articles/PMC5078801/ /pubmed/27807477 http://dx.doi.org/10.1038/celldisc.2016.38 Text en Copyright © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article L Black, Kathryn Petruk, Svetlana Fenstermaker, Tyler K Hodgson, Jacob W Caplan, Jeffrey L Brock, Hugh W Mazo, Alexander Chromatin proteins and RNA are associated with DNA during all phases of mitosis |
title | Chromatin proteins and RNA are associated with DNA during all phases of mitosis |
title_full | Chromatin proteins and RNA are associated with DNA during all phases of mitosis |
title_fullStr | Chromatin proteins and RNA are associated with DNA during all phases of mitosis |
title_full_unstemmed | Chromatin proteins and RNA are associated with DNA during all phases of mitosis |
title_short | Chromatin proteins and RNA are associated with DNA during all phases of mitosis |
title_sort | chromatin proteins and rna are associated with dna during all phases of mitosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5078801/ https://www.ncbi.nlm.nih.gov/pubmed/27807477 http://dx.doi.org/10.1038/celldisc.2016.38 |
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