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Effects of Lycium barbarum. polysaccharide on type 2 diabetes mellitus rats by regulating biological rhythms

OBJECTIVE(S): Type 2 diabetes mellitus (T2DM) is associated with circadian disruption. Our previous experimental results have showed that dietary Lycium barbarum. polysaccharide (LBP-4a) exhibited hypoglycemic and improving insulin resistance (IR) activities. This study was to explore the mechanisms...

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Autores principales: Zhao, Rui, Gao, Xu, Zhang, Tao, Li, Xing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5080419/
https://www.ncbi.nlm.nih.gov/pubmed/27803791
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author Zhao, Rui
Gao, Xu
Zhang, Tao
Li, Xing
author_facet Zhao, Rui
Gao, Xu
Zhang, Tao
Li, Xing
author_sort Zhao, Rui
collection PubMed
description OBJECTIVE(S): Type 2 diabetes mellitus (T2DM) is associated with circadian disruption. Our previous experimental results have showed that dietary Lycium barbarum. polysaccharide (LBP-4a) exhibited hypoglycemic and improving insulin resistance (IR) activities. This study was to explore the mechanisms of LBP-4a for improving hyperglycemia and IR by regulating biological rhythms in T2DM rats. MATERIALS AND METHODS: The rats of T2DM were prepared by the high-sucrose-fat diets and injection of streptozotocin (STZ). The levels of insulin, leptin and melatonin were measured by enzyme linked immunosorbent assay (ELISA). The effect of LBP-4a on mRNA expression of melatonin receptors (MT2) in epididymal adipose tissue was evaluated by RT-PCR. The expression of CLOCK and BMAL1 in pancreatic islet cells was detected by Western blotting. RESULTS: Our data indicated that the 24-hr rhythm of blood glucose appeared to have consistent with normal rats after gavaged administration of LBP-4a for each day of the 4 weeks, and the effects of hypoglycemia and improving hyperinsulinemia in T2DM rats treated at high dose were much better than that at low dose. The mechanisms were related to increasing MT2 level in epididymal adipose tissue and affecting circadian clocks gene expression of CLOCK and BMAL1 in pancreatic islet cells. CONCLUSION: LBP-4a administration could treat T2DM rats. These observations provided the background for the further development of LBP-4a as a potential dietary therapeutic agent in the treatment of T2DM.
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spelling pubmed-50804192016-11-01 Effects of Lycium barbarum. polysaccharide on type 2 diabetes mellitus rats by regulating biological rhythms Zhao, Rui Gao, Xu Zhang, Tao Li, Xing Iran J Basic Med Sci Original Article OBJECTIVE(S): Type 2 diabetes mellitus (T2DM) is associated with circadian disruption. Our previous experimental results have showed that dietary Lycium barbarum. polysaccharide (LBP-4a) exhibited hypoglycemic and improving insulin resistance (IR) activities. This study was to explore the mechanisms of LBP-4a for improving hyperglycemia and IR by regulating biological rhythms in T2DM rats. MATERIALS AND METHODS: The rats of T2DM were prepared by the high-sucrose-fat diets and injection of streptozotocin (STZ). The levels of insulin, leptin and melatonin were measured by enzyme linked immunosorbent assay (ELISA). The effect of LBP-4a on mRNA expression of melatonin receptors (MT2) in epididymal adipose tissue was evaluated by RT-PCR. The expression of CLOCK and BMAL1 in pancreatic islet cells was detected by Western blotting. RESULTS: Our data indicated that the 24-hr rhythm of blood glucose appeared to have consistent with normal rats after gavaged administration of LBP-4a for each day of the 4 weeks, and the effects of hypoglycemia and improving hyperinsulinemia in T2DM rats treated at high dose were much better than that at low dose. The mechanisms were related to increasing MT2 level in epididymal adipose tissue and affecting circadian clocks gene expression of CLOCK and BMAL1 in pancreatic islet cells. CONCLUSION: LBP-4a administration could treat T2DM rats. These observations provided the background for the further development of LBP-4a as a potential dietary therapeutic agent in the treatment of T2DM. Mashhad University of Medical Sciences 2016-09 /pmc/articles/PMC5080419/ /pubmed/27803791 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zhao, Rui
Gao, Xu
Zhang, Tao
Li, Xing
Effects of Lycium barbarum. polysaccharide on type 2 diabetes mellitus rats by regulating biological rhythms
title Effects of Lycium barbarum. polysaccharide on type 2 diabetes mellitus rats by regulating biological rhythms
title_full Effects of Lycium barbarum. polysaccharide on type 2 diabetes mellitus rats by regulating biological rhythms
title_fullStr Effects of Lycium barbarum. polysaccharide on type 2 diabetes mellitus rats by regulating biological rhythms
title_full_unstemmed Effects of Lycium barbarum. polysaccharide on type 2 diabetes mellitus rats by regulating biological rhythms
title_short Effects of Lycium barbarum. polysaccharide on type 2 diabetes mellitus rats by regulating biological rhythms
title_sort effects of lycium barbarum. polysaccharide on type 2 diabetes mellitus rats by regulating biological rhythms
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5080419/
https://www.ncbi.nlm.nih.gov/pubmed/27803791
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