Cargando…

Apoptosis induction of human endometriotic epithelial and stromal cells by noscapine

OBJECTIVE(S): Endometriosis is a complex gynecologic disease with unknown etiology. Noscapine has been introduced as a cancer cell suppressor. Endometriosis was considered as a cancer like disorder, The aim of present study was to investigate noscapine apoptotic effect on human endometriotic epithel...

Descripción completa

Detalles Bibliográficos
Autores principales: Khazaei, Mohammad Rasoul, Rashidi, Zahra, Chobsaz, Farzaneh, Khazaei, Mozafar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5080423/
https://www.ncbi.nlm.nih.gov/pubmed/27803780
_version_ 1782462710553772032
author Khazaei, Mohammad Rasoul
Rashidi, Zahra
Chobsaz, Farzaneh
Khazaei, Mozafar
author_facet Khazaei, Mohammad Rasoul
Rashidi, Zahra
Chobsaz, Farzaneh
Khazaei, Mozafar
author_sort Khazaei, Mohammad Rasoul
collection PubMed
description OBJECTIVE(S): Endometriosis is a complex gynecologic disease with unknown etiology. Noscapine has been introduced as a cancer cell suppressor. Endometriosis was considered as a cancer like disorder, The aim of present study was to investigate noscapine apoptotic effect on human endometriotic epithelial and stromal cells in vitro. MATERIALS AND METHODS: In this in vitro study, endometrial biopsies from endometriosis patients (n=9) were prepared and digested by an enzymatic method (collagenase I, 2 mg/ml). Stromal and epithelial cells were separated by sequential filtration through a cell strainer and ficoll layering. The cells of each sample were divided into five groups: control (0), 10, 25, 50 and 100 micromole/liter (µM) concentration of noscapine and were cultured for three different periods of times; 24, 48 and 72 hr. Cell viability was assessed by colorimetric assay. Nitric oxide (NO) concentration was measured by Griess reagent. Cell death was analyzed by Acridine Orange (AO)–Ethidium Bromide (EB) double staining and Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay. Data were analyzed by one-way ANOVA. RESULTS: Viability of endometrial epithelial and stromal cells significantly decreased in 10, 25, 50 and 100 µM noscapine concentration in 24, 48, 72 hr (P<0.05) and apoptotic index increased in 25, 50 and 100 µM noscapine concentrations in 48 hr significantly (P<0.05). Concentrations of NO didn’t show a significant decrease. CONCLUSION: Noscapine increased endometriotic epithelial and stromal cell death and can be suggested as a treatment for endometriosis.
format Online
Article
Text
id pubmed-5080423
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Mashhad University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-50804232016-11-01 Apoptosis induction of human endometriotic epithelial and stromal cells by noscapine Khazaei, Mohammad Rasoul Rashidi, Zahra Chobsaz, Farzaneh Khazaei, Mozafar Iran J Basic Med Sci Original Article OBJECTIVE(S): Endometriosis is a complex gynecologic disease with unknown etiology. Noscapine has been introduced as a cancer cell suppressor. Endometriosis was considered as a cancer like disorder, The aim of present study was to investigate noscapine apoptotic effect on human endometriotic epithelial and stromal cells in vitro. MATERIALS AND METHODS: In this in vitro study, endometrial biopsies from endometriosis patients (n=9) were prepared and digested by an enzymatic method (collagenase I, 2 mg/ml). Stromal and epithelial cells were separated by sequential filtration through a cell strainer and ficoll layering. The cells of each sample were divided into five groups: control (0), 10, 25, 50 and 100 micromole/liter (µM) concentration of noscapine and were cultured for three different periods of times; 24, 48 and 72 hr. Cell viability was assessed by colorimetric assay. Nitric oxide (NO) concentration was measured by Griess reagent. Cell death was analyzed by Acridine Orange (AO)–Ethidium Bromide (EB) double staining and Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay. Data were analyzed by one-way ANOVA. RESULTS: Viability of endometrial epithelial and stromal cells significantly decreased in 10, 25, 50 and 100 µM noscapine concentration in 24, 48, 72 hr (P<0.05) and apoptotic index increased in 25, 50 and 100 µM noscapine concentrations in 48 hr significantly (P<0.05). Concentrations of NO didn’t show a significant decrease. CONCLUSION: Noscapine increased endometriotic epithelial and stromal cell death and can be suggested as a treatment for endometriosis. Mashhad University of Medical Sciences 2016-09 /pmc/articles/PMC5080423/ /pubmed/27803780 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Khazaei, Mohammad Rasoul
Rashidi, Zahra
Chobsaz, Farzaneh
Khazaei, Mozafar
Apoptosis induction of human endometriotic epithelial and stromal cells by noscapine
title Apoptosis induction of human endometriotic epithelial and stromal cells by noscapine
title_full Apoptosis induction of human endometriotic epithelial and stromal cells by noscapine
title_fullStr Apoptosis induction of human endometriotic epithelial and stromal cells by noscapine
title_full_unstemmed Apoptosis induction of human endometriotic epithelial and stromal cells by noscapine
title_short Apoptosis induction of human endometriotic epithelial and stromal cells by noscapine
title_sort apoptosis induction of human endometriotic epithelial and stromal cells by noscapine
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5080423/
https://www.ncbi.nlm.nih.gov/pubmed/27803780
work_keys_str_mv AT khazaeimohammadrasoul apoptosisinductionofhumanendometrioticepithelialandstromalcellsbynoscapine
AT rashidizahra apoptosisinductionofhumanendometrioticepithelialandstromalcellsbynoscapine
AT chobsazfarzaneh apoptosisinductionofhumanendometrioticepithelialandstromalcellsbynoscapine
AT khazaeimozafar apoptosisinductionofhumanendometrioticepithelialandstromalcellsbynoscapine