Typing Method for the QUB11a Locus of Mycobacterium tuberculosis: IS6110 Insertions and Tandem Repeat Analysis

QUB11a is used as a locus for variable number of tandem repeats (VNTR) analysis of Mycobacterium tuberculosis Beijing lineage. However, amplification of QUB11a occasionally produces large fragments (>1,400 bp) that are not easily measured by capillary electrophoresis because of a lack of the typi...

Descripción completa

Detalles Bibliográficos
Autores principales: Maeda-Mitani, Eriko, Murakami, Koichi, Oishi, Akira, Etoh, Yoshiki, Sera, Nobuyuki, Fujimoto, Shuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5080463/
https://www.ncbi.nlm.nih.gov/pubmed/27812529
http://dx.doi.org/10.1155/2016/5216530
_version_ 1782462721570111488
author Maeda-Mitani, Eriko
Murakami, Koichi
Oishi, Akira
Etoh, Yoshiki
Sera, Nobuyuki
Fujimoto, Shuji
author_facet Maeda-Mitani, Eriko
Murakami, Koichi
Oishi, Akira
Etoh, Yoshiki
Sera, Nobuyuki
Fujimoto, Shuji
author_sort Maeda-Mitani, Eriko
collection PubMed
description QUB11a is used as a locus for variable number of tandem repeats (VNTR) analysis of Mycobacterium tuberculosis Beijing lineage. However, amplification of QUB11a occasionally produces large fragments (>1,400 bp) that are not easily measured by capillary electrophoresis because of a lack of the typical stutter peak patterns that are used for counting repeat numbers. IS6110 insertion may complicate VNTR analysis of large QUB11a fragments in M. tuberculosis. We established a method for determining both tandem repeat numbers and IS6110 insertion in the QUB11a locus of M. tuberculosis using capillary electrophoresis analysis and BsmBI digestion. All 29 large QUB11a fragments (>1,200 bp) investigated contained IS6110 insertions and varied in the number of repeats (18 patterns) and location of IS6110 insertions. This method allows VNTR analysis with high discrimination.
format Online
Article
Text
id pubmed-5080463
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-50804632016-11-03 Typing Method for the QUB11a Locus of Mycobacterium tuberculosis: IS6110 Insertions and Tandem Repeat Analysis Maeda-Mitani, Eriko Murakami, Koichi Oishi, Akira Etoh, Yoshiki Sera, Nobuyuki Fujimoto, Shuji Biomed Res Int Research Article QUB11a is used as a locus for variable number of tandem repeats (VNTR) analysis of Mycobacterium tuberculosis Beijing lineage. However, amplification of QUB11a occasionally produces large fragments (>1,400 bp) that are not easily measured by capillary electrophoresis because of a lack of the typical stutter peak patterns that are used for counting repeat numbers. IS6110 insertion may complicate VNTR analysis of large QUB11a fragments in M. tuberculosis. We established a method for determining both tandem repeat numbers and IS6110 insertion in the QUB11a locus of M. tuberculosis using capillary electrophoresis analysis and BsmBI digestion. All 29 large QUB11a fragments (>1,200 bp) investigated contained IS6110 insertions and varied in the number of repeats (18 patterns) and location of IS6110 insertions. This method allows VNTR analysis with high discrimination. Hindawi Publishing Corporation 2016 2016-10-16 /pmc/articles/PMC5080463/ /pubmed/27812529 http://dx.doi.org/10.1155/2016/5216530 Text en Copyright © 2016 Eriko Maeda-Mitani et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Maeda-Mitani, Eriko
Murakami, Koichi
Oishi, Akira
Etoh, Yoshiki
Sera, Nobuyuki
Fujimoto, Shuji
Typing Method for the QUB11a Locus of Mycobacterium tuberculosis: IS6110 Insertions and Tandem Repeat Analysis
title Typing Method for the QUB11a Locus of Mycobacterium tuberculosis: IS6110 Insertions and Tandem Repeat Analysis
title_full Typing Method for the QUB11a Locus of Mycobacterium tuberculosis: IS6110 Insertions and Tandem Repeat Analysis
title_fullStr Typing Method for the QUB11a Locus of Mycobacterium tuberculosis: IS6110 Insertions and Tandem Repeat Analysis
title_full_unstemmed Typing Method for the QUB11a Locus of Mycobacterium tuberculosis: IS6110 Insertions and Tandem Repeat Analysis
title_short Typing Method for the QUB11a Locus of Mycobacterium tuberculosis: IS6110 Insertions and Tandem Repeat Analysis
title_sort typing method for the qub11a locus of mycobacterium tuberculosis: is6110 insertions and tandem repeat analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5080463/
https://www.ncbi.nlm.nih.gov/pubmed/27812529
http://dx.doi.org/10.1155/2016/5216530
work_keys_str_mv AT maedamitanieriko typingmethodforthequb11alocusofmycobacteriumtuberculosisis6110insertionsandtandemrepeatanalysis
AT murakamikoichi typingmethodforthequb11alocusofmycobacteriumtuberculosisis6110insertionsandtandemrepeatanalysis
AT oishiakira typingmethodforthequb11alocusofmycobacteriumtuberculosisis6110insertionsandtandemrepeatanalysis
AT etohyoshiki typingmethodforthequb11alocusofmycobacteriumtuberculosisis6110insertionsandtandemrepeatanalysis
AT seranobuyuki typingmethodforthequb11alocusofmycobacteriumtuberculosisis6110insertionsandtandemrepeatanalysis
AT fujimotoshuji typingmethodforthequb11alocusofmycobacteriumtuberculosisis6110insertionsandtandemrepeatanalysis

Ejemplares similares