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Expression of varied GFPs in Saccharomyces cerevisiae: codon optimization yields stronger than expected expression and fluorescence intensity

Green fluorescent protein (GFP), which was originally isolated from jellyfish, is a widely used tool in biological research, and homologs from other organisms are available. However, researchers must determine which GFP is the most suitable for a specific host. Here, we expressed GFPs from several s...

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Detalles Bibliográficos
Autores principales: Kaishima, Misato, Ishii, Jun, Matsuno, Toshihide, Fukuda, Nobuo, Kondo, Akihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5080575/
https://www.ncbi.nlm.nih.gov/pubmed/27782154
http://dx.doi.org/10.1038/srep35932

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