Cargando…
Expression of varied GFPs in Saccharomyces cerevisiae: codon optimization yields stronger than expected expression and fluorescence intensity
Green fluorescent protein (GFP), which was originally isolated from jellyfish, is a widely used tool in biological research, and homologs from other organisms are available. However, researchers must determine which GFP is the most suitable for a specific host. Here, we expressed GFPs from several s...
Autores principales: | Kaishima, Misato, Ishii, Jun, Matsuno, Toshihide, Fukuda, Nobuo, Kondo, Akihiko |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5080575/ https://www.ncbi.nlm.nih.gov/pubmed/27782154 http://dx.doi.org/10.1038/srep35932 |
Ejemplares similares
-
Desired Alteration of Protein Affinities: Competitive Selection of Protein Variants Using Yeast Signal Transduction Machinery
por: Kaishima, Misato, et al.
Publicado: (2014) -
Gγ recruitment systems specifically select PPI and affinity-enhanced candidate proteins that interact with membrane protein targets
por: Kaishima, Misato, et al.
Publicado: (2015) -
Continuous crossbreeding of sake yeasts using growth selection systems for a-type and α-type cells
por: Fukuda, Nobuo, et al.
Publicado: (2016) -
Synthetic Biology of Proteins: Tuning GFPs Folding and Stability with Fluoroproline
por: Steiner, Thomas, et al.
Publicado: (2008) -
Identification of stop codon readthrough genes in Saccharomyces cerevisiae
por: Namy, Olivier, et al.
Publicado: (2003)