Super-resolution imaging of fluorescently labeled, endogenous RNA Polymerase II in living cells with CRISPR/Cas9-mediated gene editing

Live cell imaging of mammalian RNA polymerase II (Pol II) has previously relied on random insertions of exogenous, mutant Pol II coupled with the degradation of endogenous Pol II using a toxin, α-amanitin. Therefore, it has been unclear whether over-expression of labeled Pol II under an exogenous pr...

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Detalles Bibliográficos
Autores principales: Cho, Won-Ki, Jayanth, Namrata, Mullen, Susan, Tan, Tzer Han, Jung, Yoon J., Cissé, Ibrahim I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5080603/
https://www.ncbi.nlm.nih.gov/pubmed/27782203
http://dx.doi.org/10.1038/srep35949

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