Fluorescent protein tagging of endogenous protein in brain neurons using CRISPR/Cas9-mediated knock-in and in utero electroporation techniques

Genome editing is a powerful technique for studying gene functions. CRISPR/Cas9-mediated gene knock-in has recently been applied to various cells and organisms. Here, we successfully knocked in an EGFP coding sequence at the site immediately after the first ATG codon of the β-actin gene in neurons i...

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Detalles Bibliográficos
Autores principales: Uemura, Takeshi, Mori, Takuma, Kurihara, Taiga, Kawase, Shiori, Koike, Rie, Satoga, Michiru, Cao, Xueshan, Li, Xue, Yanagawa, Toru, Sakurai, Takayuki, Shindo, Takayuki, Tabuchi, Katsuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5080626/
https://www.ncbi.nlm.nih.gov/pubmed/27782168
http://dx.doi.org/10.1038/srep35861

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