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Diagnostic utility of Wilms’ tumour-1 protein (WT-1) immunostaining in paediatric renal tumours

BACKGROUND & OBJECTIVES: Renal tumours constitute about 7 per cent of all neoplasms in children. It is important to differentiate Wilms’ tumour (commonest tumour) from non-Wilms’ tumours. The aim of this study was to evaluate the immunoexpression and diagnostic role of Wilms’ tumour-1 protein (W...

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Autores principales: Goyal, Surbhi, Mishra, Kiran, Sarkar, Urvee, Sharma, Satendra, Kumari, Anita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5080930/
https://www.ncbi.nlm.nih.gov/pubmed/27748279
http://dx.doi.org/10.4103/0971-5916.191776
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author Goyal, Surbhi
Mishra, Kiran
Sarkar, Urvee
Sharma, Satendra
Kumari, Anita
author_facet Goyal, Surbhi
Mishra, Kiran
Sarkar, Urvee
Sharma, Satendra
Kumari, Anita
author_sort Goyal, Surbhi
collection PubMed
description BACKGROUND & OBJECTIVES: Renal tumours constitute about 7 per cent of all neoplasms in children. It is important to differentiate Wilms’ tumour (commonest tumour) from non-Wilms’ tumours. The aim of this study was to evaluate the immunoexpression and diagnostic role of Wilms’ tumour-1 protein (WT1) in paediatric renal tumours. METHODS: A total of 53 cases of renal tumours in children (below 18 yr) who underwent total nephrectomy were included in this retrospective study. WT1 immunostaining was done using mouse monoclonal WT1 antibody (clone: 6F-H2). RESULTS: Of the 53 cases, 38 (72%) were of Wilms’ tumour. Non-Wilms’ group (15) included six cases of mesoblastic nephroma (MN), two each of clear cell sarcoma (CCSK), renal cell carcinoma (RCC) and peripheral neuroectodermal tumour (PNET) and one each of angiomyolipoma (AML), rhabdomyosarcoma (RMS) and malignant rhabdoid tumour (MRT). Proportion of WT1 positivity in Wilms’ tumour was 100 per cent in contrast to 26.7 per cent in non-Wilms’ tumours (P<0.001). Epithelial and blastemal components of Wilms’ tumour showed moderate (2+) nuclear and cytoplasmic staining in 80 (24/30) and 75 per cent (24/32) cases, respectively. MN, PNET, CCSK and AML were negative for WT1. RMS, RCC and MRT showed cytoplasmic staining, strongest in RMS. No significant association was seen between WT1 expression and NWTSG (National Wilms’ Tumor Study Group) stage. INTERPRETATION & CONCLUSIONS: WT1 helps to differentiate Wilms’ tumour from other paediatric renal tumours. It may help in differentiating the two subgroups of Wilms’ tumour which have distinct molecular pathogenesis and biological behaviour, however, further prospective studies are required for validation of this hypothesis.
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spelling pubmed-50809302016-11-14 Diagnostic utility of Wilms’ tumour-1 protein (WT-1) immunostaining in paediatric renal tumours Goyal, Surbhi Mishra, Kiran Sarkar, Urvee Sharma, Satendra Kumari, Anita Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Renal tumours constitute about 7 per cent of all neoplasms in children. It is important to differentiate Wilms’ tumour (commonest tumour) from non-Wilms’ tumours. The aim of this study was to evaluate the immunoexpression and diagnostic role of Wilms’ tumour-1 protein (WT1) in paediatric renal tumours. METHODS: A total of 53 cases of renal tumours in children (below 18 yr) who underwent total nephrectomy were included in this retrospective study. WT1 immunostaining was done using mouse monoclonal WT1 antibody (clone: 6F-H2). RESULTS: Of the 53 cases, 38 (72%) were of Wilms’ tumour. Non-Wilms’ group (15) included six cases of mesoblastic nephroma (MN), two each of clear cell sarcoma (CCSK), renal cell carcinoma (RCC) and peripheral neuroectodermal tumour (PNET) and one each of angiomyolipoma (AML), rhabdomyosarcoma (RMS) and malignant rhabdoid tumour (MRT). Proportion of WT1 positivity in Wilms’ tumour was 100 per cent in contrast to 26.7 per cent in non-Wilms’ tumours (P<0.001). Epithelial and blastemal components of Wilms’ tumour showed moderate (2+) nuclear and cytoplasmic staining in 80 (24/30) and 75 per cent (24/32) cases, respectively. MN, PNET, CCSK and AML were negative for WT1. RMS, RCC and MRT showed cytoplasmic staining, strongest in RMS. No significant association was seen between WT1 expression and NWTSG (National Wilms’ Tumor Study Group) stage. INTERPRETATION & CONCLUSIONS: WT1 helps to differentiate Wilms’ tumour from other paediatric renal tumours. It may help in differentiating the two subgroups of Wilms’ tumour which have distinct molecular pathogenesis and biological behaviour, however, further prospective studies are required for validation of this hypothesis. Medknow Publications & Media Pvt Ltd 2016-05 /pmc/articles/PMC5080930/ /pubmed/27748279 http://dx.doi.org/10.4103/0971-5916.191776 Text en Copyright: © Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Goyal, Surbhi
Mishra, Kiran
Sarkar, Urvee
Sharma, Satendra
Kumari, Anita
Diagnostic utility of Wilms’ tumour-1 protein (WT-1) immunostaining in paediatric renal tumours
title Diagnostic utility of Wilms’ tumour-1 protein (WT-1) immunostaining in paediatric renal tumours
title_full Diagnostic utility of Wilms’ tumour-1 protein (WT-1) immunostaining in paediatric renal tumours
title_fullStr Diagnostic utility of Wilms’ tumour-1 protein (WT-1) immunostaining in paediatric renal tumours
title_full_unstemmed Diagnostic utility of Wilms’ tumour-1 protein (WT-1) immunostaining in paediatric renal tumours
title_short Diagnostic utility of Wilms’ tumour-1 protein (WT-1) immunostaining in paediatric renal tumours
title_sort diagnostic utility of wilms’ tumour-1 protein (wt-1) immunostaining in paediatric renal tumours
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5080930/
https://www.ncbi.nlm.nih.gov/pubmed/27748279
http://dx.doi.org/10.4103/0971-5916.191776
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