Antioxidation of Cerium Oxide Nanoparticles to Several Series of Oxidative Damage Related to Type II Diabetes Mellitus In Vitro

BACKGROUND: It is well known that cerium oxide nanoparticles (CeNPs) have intense antioxidant activity. The antioxidant property of CeNPs are widely used in different areas of research, but little is known about the oxidative damage of Cu(2+) associated with Type II diabetes mellitus (T2DM). MATERIAL/METHODS: In our research, the function of CeNPs was tested for its protection of β-cells from the damage of Cu(2+) or H(2)O(2). We detected hydroxyl radicals using terephthalic acid assay, hydrogen peroxide using Amplex Ultra Red assay, and cell viability using MTT reduction. RESULTS: We found that CeNPs can persistently inhibit Cu(2+)/H(2)O(2) evoked hydroxyl radicals and hydrogen peroxide in oxidative stress of β-cells. CONCLUSIONS: CeNPs will be useful in developing strategies for the prevention of T2DM.