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Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering
Concentrations of S-Adenosyl-L-Methionine (SAM) in aqueous solution and fermentation liquids were quantitatively determined by surface-enhanced Raman scattering (SERS) and verified by high-pressure liquid chromatography (HPLC). The Ag nanoparticle/silicon nanowire array substrate was fabricated and...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081456/ https://www.ncbi.nlm.nih.gov/pubmed/27818834 http://dx.doi.org/10.1155/2016/4910630 |
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author | Ren, Hairui Chen, Zhaoyang Zhang, Xin Zhao, Yongmei Wang, Zheng Wu, Zhenglong Xu, Haijun |
author_facet | Ren, Hairui Chen, Zhaoyang Zhang, Xin Zhao, Yongmei Wang, Zheng Wu, Zhenglong Xu, Haijun |
author_sort | Ren, Hairui |
collection | PubMed |
description | Concentrations of S-Adenosyl-L-Methionine (SAM) in aqueous solution and fermentation liquids were quantitatively determined by surface-enhanced Raman scattering (SERS) and verified by high-pressure liquid chromatography (HPLC). The Ag nanoparticle/silicon nanowire array substrate was fabricated and employed as an active SERS substrate to indirectly measure the SAM concentration. The linear relationship between the integrated intensity of peak centered at ~2920 cm(−1) in SERS spectra and the SAM concentration was established, and the limit of detections of SAM concentrations was analyzed to be ~0.1 g/L. The concentration of SAM in real solution could be predicted by the linear relationship and verified by the HPLC detection method. The relative deviations (δ) of the predicted SAM concentration are less than 13% and the correlation coefficient is 0.9998. Rolling-Circle Filter was utilized to subtract fluorescence background and the optimal results were obtained when the radius of the analyzing circle is 650 cm(−1). |
format | Online Article Text |
id | pubmed-5081456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-50814562016-11-06 Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering Ren, Hairui Chen, Zhaoyang Zhang, Xin Zhao, Yongmei Wang, Zheng Wu, Zhenglong Xu, Haijun J Anal Methods Chem Research Article Concentrations of S-Adenosyl-L-Methionine (SAM) in aqueous solution and fermentation liquids were quantitatively determined by surface-enhanced Raman scattering (SERS) and verified by high-pressure liquid chromatography (HPLC). The Ag nanoparticle/silicon nanowire array substrate was fabricated and employed as an active SERS substrate to indirectly measure the SAM concentration. The linear relationship between the integrated intensity of peak centered at ~2920 cm(−1) in SERS spectra and the SAM concentration was established, and the limit of detections of SAM concentrations was analyzed to be ~0.1 g/L. The concentration of SAM in real solution could be predicted by the linear relationship and verified by the HPLC detection method. The relative deviations (δ) of the predicted SAM concentration are less than 13% and the correlation coefficient is 0.9998. Rolling-Circle Filter was utilized to subtract fluorescence background and the optimal results were obtained when the radius of the analyzing circle is 650 cm(−1). Hindawi Publishing Corporation 2016 2016-10-13 /pmc/articles/PMC5081456/ /pubmed/27818834 http://dx.doi.org/10.1155/2016/4910630 Text en Copyright © 2016 Hairui Ren et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ren, Hairui Chen, Zhaoyang Zhang, Xin Zhao, Yongmei Wang, Zheng Wu, Zhenglong Xu, Haijun Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering |
title | Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering |
title_full | Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering |
title_fullStr | Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering |
title_full_unstemmed | Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering |
title_short | Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering |
title_sort | rapid and quantitative determination of s-adenosyl-l-methionine in the fermentation process by surface-enhanced raman scattering |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081456/ https://www.ncbi.nlm.nih.gov/pubmed/27818834 http://dx.doi.org/10.1155/2016/4910630 |
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