Cargando…

Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus

Based on the galactinol synthase (AnGolS1) fragment sequence from a cold-induced Suppression Subtractive Hybridization (SSH) library derived from Ammopiptanthus nanus (A. nanus) seedlings, AnGolS1 mRNA (including the 5′ UTR and 3′ UTR) (GenBank accession number: GU942748) was isolated and characteri...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, YuDong, Zhang, Li, Chen, LiJing, Ma, Hui, Ruan, YanYe, Xu, Tao, Xu, ChuanQiang, He, Yi, Qi, MingFang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081558/
https://www.ncbi.nlm.nih.gov/pubmed/27786294
http://dx.doi.org/10.1038/srep36113
_version_ 1782462921485320192
author Liu, YuDong
Zhang, Li
Chen, LiJing
Ma, Hui
Ruan, YanYe
Xu, Tao
Xu, ChuanQiang
He, Yi
Qi, MingFang
author_facet Liu, YuDong
Zhang, Li
Chen, LiJing
Ma, Hui
Ruan, YanYe
Xu, Tao
Xu, ChuanQiang
He, Yi
Qi, MingFang
author_sort Liu, YuDong
collection PubMed
description Based on the galactinol synthase (AnGolS1) fragment sequence from a cold-induced Suppression Subtractive Hybridization (SSH) library derived from Ammopiptanthus nanus (A. nanus) seedlings, AnGolS1 mRNA (including the 5′ UTR and 3′ UTR) (GenBank accession number: GU942748) was isolated and characterized by rapid amplification of cDNA ends polymerase chain reaction (RACE–PCR). A substrate reaction test revealed that AnGolS1 possessed galactinol synthase activity in vitro and could potentially be an early-responsive gene. Furthermore, quantitative real-time PCR (qRT-PCR) indicated that AnGolS1 was responded to cold, salts and drought stresses, however, significantly up-regulated in all origans by low temperatures, especially in plant stems. In addition, the hybridization signals in the fascicular cambium were strongest in all cells under low temperature. Thus, we propose that AnGolS1 plays critical roles in A. nanus low-temperature stress resistance and that fascicular cambium cells could be involved in AnGolS1 mRNA transcription, galactinol transportation and coordination under low-temperature stress.
format Online
Article
Text
id pubmed-5081558
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-50815582016-10-31 Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus Liu, YuDong Zhang, Li Chen, LiJing Ma, Hui Ruan, YanYe Xu, Tao Xu, ChuanQiang He, Yi Qi, MingFang Sci Rep Article Based on the galactinol synthase (AnGolS1) fragment sequence from a cold-induced Suppression Subtractive Hybridization (SSH) library derived from Ammopiptanthus nanus (A. nanus) seedlings, AnGolS1 mRNA (including the 5′ UTR and 3′ UTR) (GenBank accession number: GU942748) was isolated and characterized by rapid amplification of cDNA ends polymerase chain reaction (RACE–PCR). A substrate reaction test revealed that AnGolS1 possessed galactinol synthase activity in vitro and could potentially be an early-responsive gene. Furthermore, quantitative real-time PCR (qRT-PCR) indicated that AnGolS1 was responded to cold, salts and drought stresses, however, significantly up-regulated in all origans by low temperatures, especially in plant stems. In addition, the hybridization signals in the fascicular cambium were strongest in all cells under low temperature. Thus, we propose that AnGolS1 plays critical roles in A. nanus low-temperature stress resistance and that fascicular cambium cells could be involved in AnGolS1 mRNA transcription, galactinol transportation and coordination under low-temperature stress. Nature Publishing Group 2016-10-27 /pmc/articles/PMC5081558/ /pubmed/27786294 http://dx.doi.org/10.1038/srep36113 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Liu, YuDong
Zhang, Li
Chen, LiJing
Ma, Hui
Ruan, YanYe
Xu, Tao
Xu, ChuanQiang
He, Yi
Qi, MingFang
Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus
title Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus
title_full Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus
title_fullStr Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus
title_full_unstemmed Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus
title_short Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus
title_sort molecular cloning and expression of an encoding galactinol synthase gene (angols1) in seedling of ammopiptanthus nanus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081558/
https://www.ncbi.nlm.nih.gov/pubmed/27786294
http://dx.doi.org/10.1038/srep36113
work_keys_str_mv AT liuyudong molecularcloningandexpressionofanencodinggalactinolsynthasegeneangols1inseedlingofammopiptanthusnanus
AT zhangli molecularcloningandexpressionofanencodinggalactinolsynthasegeneangols1inseedlingofammopiptanthusnanus
AT chenlijing molecularcloningandexpressionofanencodinggalactinolsynthasegeneangols1inseedlingofammopiptanthusnanus
AT mahui molecularcloningandexpressionofanencodinggalactinolsynthasegeneangols1inseedlingofammopiptanthusnanus
AT ruanyanye molecularcloningandexpressionofanencodinggalactinolsynthasegeneangols1inseedlingofammopiptanthusnanus
AT xutao molecularcloningandexpressionofanencodinggalactinolsynthasegeneangols1inseedlingofammopiptanthusnanus
AT xuchuanqiang molecularcloningandexpressionofanencodinggalactinolsynthasegeneangols1inseedlingofammopiptanthusnanus
AT heyi molecularcloningandexpressionofanencodinggalactinolsynthasegeneangols1inseedlingofammopiptanthusnanus
AT qimingfang molecularcloningandexpressionofanencodinggalactinolsynthasegeneangols1inseedlingofammopiptanthusnanus