Cargando…
A transgenic-cloned pig model expressing non-fluorescent modified Plum
Genetically modified pigs that express fluorescent proteins such as green and red fluorescent proteins have become indispensable biomedical research tools in recent years. Cell or tissue transplantation studies using fluorescent markers should be conducted, wherein the xeno-antigenicity of the fluor...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society for Reproduction and Development
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081739/ https://www.ncbi.nlm.nih.gov/pubmed/27396383 http://dx.doi.org/10.1262/jrd.2016-041 |
_version_ | 1782462941531996160 |
---|---|
author | NAGAYA, Masaki WATANABE, Masahito KOBAYASHI, Mirina NAKANO, Kazuaki ARAI, Yoshikazu ASANO, Yoshinori TAKEISHI, Toki UMEKI, Ikuma FUKUDA, Tooru YASHIMA, Sayaka TAKAYANAGI, Shuko WATANABE, Nobuyuki ONODERA, Masafumi MATSUNARI, Hitomi UMEYAMA, Kazuhiro NAGASHIMA, Hiroshi |
author_facet | NAGAYA, Masaki WATANABE, Masahito KOBAYASHI, Mirina NAKANO, Kazuaki ARAI, Yoshikazu ASANO, Yoshinori TAKEISHI, Toki UMEKI, Ikuma FUKUDA, Tooru YASHIMA, Sayaka TAKAYANAGI, Shuko WATANABE, Nobuyuki ONODERA, Masafumi MATSUNARI, Hitomi UMEYAMA, Kazuhiro NAGASHIMA, Hiroshi |
author_sort | NAGAYA, Masaki |
collection | PubMed |
description | Genetically modified pigs that express fluorescent proteins such as green and red fluorescent proteins have become indispensable biomedical research tools in recent years. Cell or tissue transplantation studies using fluorescent markers should be conducted, wherein the xeno-antigenicity of the fluorescent proteins does not affect engraftment or graft survival. Thus, we aimed to create a transgenic (Tg)-cloned pig that was immunologically tolerant to fluorescent protein antigens. In the present study, we generated a Tg-cloned pig harboring a derivative of Plum modified by a single amino acid substitution in the chromophore. The cells and tissues of this Tg-cloned pig expressing the modified Plum (mPlum) did not fluoresce. However, western blot and immunohistochemistry analyses clearly showed that the mPlum had the same antigenicity as Plum. Thus, we have obtained primary proof of principle for creating a cloned pig that is immunologically tolerant to fluorescent protein antigens. |
format | Online Article Text |
id | pubmed-5081739 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Society for Reproduction and Development |
record_format | MEDLINE/PubMed |
spelling | pubmed-50817392016-10-28 A transgenic-cloned pig model expressing non-fluorescent modified Plum NAGAYA, Masaki WATANABE, Masahito KOBAYASHI, Mirina NAKANO, Kazuaki ARAI, Yoshikazu ASANO, Yoshinori TAKEISHI, Toki UMEKI, Ikuma FUKUDA, Tooru YASHIMA, Sayaka TAKAYANAGI, Shuko WATANABE, Nobuyuki ONODERA, Masafumi MATSUNARI, Hitomi UMEYAMA, Kazuhiro NAGASHIMA, Hiroshi J Reprod Dev Original Article Genetically modified pigs that express fluorescent proteins such as green and red fluorescent proteins have become indispensable biomedical research tools in recent years. Cell or tissue transplantation studies using fluorescent markers should be conducted, wherein the xeno-antigenicity of the fluorescent proteins does not affect engraftment or graft survival. Thus, we aimed to create a transgenic (Tg)-cloned pig that was immunologically tolerant to fluorescent protein antigens. In the present study, we generated a Tg-cloned pig harboring a derivative of Plum modified by a single amino acid substitution in the chromophore. The cells and tissues of this Tg-cloned pig expressing the modified Plum (mPlum) did not fluoresce. However, western blot and immunohistochemistry analyses clearly showed that the mPlum had the same antigenicity as Plum. Thus, we have obtained primary proof of principle for creating a cloned pig that is immunologically tolerant to fluorescent protein antigens. The Society for Reproduction and Development 2016-07-11 2016-10 /pmc/articles/PMC5081739/ /pubmed/27396383 http://dx.doi.org/10.1262/jrd.2016-041 Text en ©2016 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Original Article NAGAYA, Masaki WATANABE, Masahito KOBAYASHI, Mirina NAKANO, Kazuaki ARAI, Yoshikazu ASANO, Yoshinori TAKEISHI, Toki UMEKI, Ikuma FUKUDA, Tooru YASHIMA, Sayaka TAKAYANAGI, Shuko WATANABE, Nobuyuki ONODERA, Masafumi MATSUNARI, Hitomi UMEYAMA, Kazuhiro NAGASHIMA, Hiroshi A transgenic-cloned pig model expressing non-fluorescent modified Plum |
title | A transgenic-cloned pig model expressing non-fluorescent modified Plum |
title_full | A transgenic-cloned pig model expressing non-fluorescent modified Plum |
title_fullStr | A transgenic-cloned pig model expressing non-fluorescent modified Plum |
title_full_unstemmed | A transgenic-cloned pig model expressing non-fluorescent modified Plum |
title_short | A transgenic-cloned pig model expressing non-fluorescent modified Plum |
title_sort | transgenic-cloned pig model expressing non-fluorescent modified plum |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081739/ https://www.ncbi.nlm.nih.gov/pubmed/27396383 http://dx.doi.org/10.1262/jrd.2016-041 |
work_keys_str_mv | AT nagayamasaki atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT watanabemasahito atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT kobayashimirina atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT nakanokazuaki atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT araiyoshikazu atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT asanoyoshinori atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT takeishitoki atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT umekiikuma atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT fukudatooru atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT yashimasayaka atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT takayanagishuko atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT watanabenobuyuki atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT onoderamasafumi atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT matsunarihitomi atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT umeyamakazuhiro atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT nagashimahiroshi atransgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT nagayamasaki transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT watanabemasahito transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT kobayashimirina transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT nakanokazuaki transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT araiyoshikazu transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT asanoyoshinori transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT takeishitoki transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT umekiikuma transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT fukudatooru transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT yashimasayaka transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT takayanagishuko transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT watanabenobuyuki transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT onoderamasafumi transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT matsunarihitomi transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT umeyamakazuhiro transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum AT nagashimahiroshi transgenicclonedpigmodelexpressingnonfluorescentmodifiedplum |