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A transgenic-cloned pig model expressing non-fluorescent modified Plum

Genetically modified pigs that express fluorescent proteins such as green and red fluorescent proteins have become indispensable biomedical research tools in recent years. Cell or tissue transplantation studies using fluorescent markers should be conducted, wherein the xeno-antigenicity of the fluor...

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Autores principales: NAGAYA, Masaki, WATANABE, Masahito, KOBAYASHI, Mirina, NAKANO, Kazuaki, ARAI, Yoshikazu, ASANO, Yoshinori, TAKEISHI, Toki, UMEKI, Ikuma, FUKUDA, Tooru, YASHIMA, Sayaka, TAKAYANAGI, Shuko, WATANABE, Nobuyuki, ONODERA, Masafumi, MATSUNARI, Hitomi, UMEYAMA, Kazuhiro, NAGASHIMA, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081739/
https://www.ncbi.nlm.nih.gov/pubmed/27396383
http://dx.doi.org/10.1262/jrd.2016-041
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author NAGAYA, Masaki
WATANABE, Masahito
KOBAYASHI, Mirina
NAKANO, Kazuaki
ARAI, Yoshikazu
ASANO, Yoshinori
TAKEISHI, Toki
UMEKI, Ikuma
FUKUDA, Tooru
YASHIMA, Sayaka
TAKAYANAGI, Shuko
WATANABE, Nobuyuki
ONODERA, Masafumi
MATSUNARI, Hitomi
UMEYAMA, Kazuhiro
NAGASHIMA, Hiroshi
author_facet NAGAYA, Masaki
WATANABE, Masahito
KOBAYASHI, Mirina
NAKANO, Kazuaki
ARAI, Yoshikazu
ASANO, Yoshinori
TAKEISHI, Toki
UMEKI, Ikuma
FUKUDA, Tooru
YASHIMA, Sayaka
TAKAYANAGI, Shuko
WATANABE, Nobuyuki
ONODERA, Masafumi
MATSUNARI, Hitomi
UMEYAMA, Kazuhiro
NAGASHIMA, Hiroshi
author_sort NAGAYA, Masaki
collection PubMed
description Genetically modified pigs that express fluorescent proteins such as green and red fluorescent proteins have become indispensable biomedical research tools in recent years. Cell or tissue transplantation studies using fluorescent markers should be conducted, wherein the xeno-antigenicity of the fluorescent proteins does not affect engraftment or graft survival. Thus, we aimed to create a transgenic (Tg)-cloned pig that was immunologically tolerant to fluorescent protein antigens. In the present study, we generated a Tg-cloned pig harboring a derivative of Plum modified by a single amino acid substitution in the chromophore. The cells and tissues of this Tg-cloned pig expressing the modified Plum (mPlum) did not fluoresce. However, western blot and immunohistochemistry analyses clearly showed that the mPlum had the same antigenicity as Plum. Thus, we have obtained primary proof of principle for creating a cloned pig that is immunologically tolerant to fluorescent protein antigens.
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spelling pubmed-50817392016-10-28 A transgenic-cloned pig model expressing non-fluorescent modified Plum NAGAYA, Masaki WATANABE, Masahito KOBAYASHI, Mirina NAKANO, Kazuaki ARAI, Yoshikazu ASANO, Yoshinori TAKEISHI, Toki UMEKI, Ikuma FUKUDA, Tooru YASHIMA, Sayaka TAKAYANAGI, Shuko WATANABE, Nobuyuki ONODERA, Masafumi MATSUNARI, Hitomi UMEYAMA, Kazuhiro NAGASHIMA, Hiroshi J Reprod Dev Original Article Genetically modified pigs that express fluorescent proteins such as green and red fluorescent proteins have become indispensable biomedical research tools in recent years. Cell or tissue transplantation studies using fluorescent markers should be conducted, wherein the xeno-antigenicity of the fluorescent proteins does not affect engraftment or graft survival. Thus, we aimed to create a transgenic (Tg)-cloned pig that was immunologically tolerant to fluorescent protein antigens. In the present study, we generated a Tg-cloned pig harboring a derivative of Plum modified by a single amino acid substitution in the chromophore. The cells and tissues of this Tg-cloned pig expressing the modified Plum (mPlum) did not fluoresce. However, western blot and immunohistochemistry analyses clearly showed that the mPlum had the same antigenicity as Plum. Thus, we have obtained primary proof of principle for creating a cloned pig that is immunologically tolerant to fluorescent protein antigens. The Society for Reproduction and Development 2016-07-11 2016-10 /pmc/articles/PMC5081739/ /pubmed/27396383 http://dx.doi.org/10.1262/jrd.2016-041 Text en ©2016 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original Article
NAGAYA, Masaki
WATANABE, Masahito
KOBAYASHI, Mirina
NAKANO, Kazuaki
ARAI, Yoshikazu
ASANO, Yoshinori
TAKEISHI, Toki
UMEKI, Ikuma
FUKUDA, Tooru
YASHIMA, Sayaka
TAKAYANAGI, Shuko
WATANABE, Nobuyuki
ONODERA, Masafumi
MATSUNARI, Hitomi
UMEYAMA, Kazuhiro
NAGASHIMA, Hiroshi
A transgenic-cloned pig model expressing non-fluorescent modified Plum
title A transgenic-cloned pig model expressing non-fluorescent modified Plum
title_full A transgenic-cloned pig model expressing non-fluorescent modified Plum
title_fullStr A transgenic-cloned pig model expressing non-fluorescent modified Plum
title_full_unstemmed A transgenic-cloned pig model expressing non-fluorescent modified Plum
title_short A transgenic-cloned pig model expressing non-fluorescent modified Plum
title_sort transgenic-cloned pig model expressing non-fluorescent modified plum
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081739/
https://www.ncbi.nlm.nih.gov/pubmed/27396383
http://dx.doi.org/10.1262/jrd.2016-041
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