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In vitro maturation of immature rat oocytes under simple culture conditions and subsequent developmental ability

Rat oocytes can be produced artificially by superovulation. Because some strains show low sensitivity to superovulation treatment, in vitro maturation is an alternative method to produce numerous matured oocytes. Furthermore, establishment of an in vitro maturation system with simple culture conditi...

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Autores principales: TAKETSURU, Hiroaki, KANEKO, Takehito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081740/
https://www.ncbi.nlm.nih.gov/pubmed/27375268
http://dx.doi.org/10.1262/jrd.2016-057
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author TAKETSURU, Hiroaki
KANEKO, Takehito
author_facet TAKETSURU, Hiroaki
KANEKO, Takehito
author_sort TAKETSURU, Hiroaki
collection PubMed
description Rat oocytes can be produced artificially by superovulation. Because some strains show low sensitivity to superovulation treatment, in vitro maturation is an alternative method to produce numerous matured oocytes. Furthermore, establishment of an in vitro maturation system with simple culture conditions is cost effective and leads to easy handling of oocytes. This study examined developmental ability of rat germinal vesicle (GV) oocytes maturing in vitro under simple culture conditions. Significantly different numbers of ovulated oocytes reached the second metaphase of meiosis (MII) among Jcl:Wistar (17.0), F344/Stm (31.0), and BN/SsNSlc (2.2) rats in whom superovulation was induced by pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin. However, similar numbers of GV oocytes were obtained from ovaries of PMSG-injected Wistar (27.7), F344 (34.7), and BN (24.7) rats. These GV oocytes were cultured in vitro in HTF, αMEM, and a 1:1 HTF + αMEM or TYH + αMEM mixture. High proportions of Wistar and F344 oocytes that matured to MII in αMEM were parthenogenetically activated by strontium chloride treatment (78% and 74%, respectively). Additionally, 10% of matured oocytes of both strains developed into offspring after intracytoplasmic sperm injection and embryo transfer to foster mothers. Although BN oocytes cultured in αMEM could be parthenogenetically activated and developed into offspring, the success rate was lower than that for Wistar and F344 oocytes. This study demonstrated that numerous GV oocytes were produced in rat ovaries by PMSG injection. This simple in vitro maturation system of immature oocytes could be further developed to maintain valuable rat strains experiencing reproductive difficulties.
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spelling pubmed-50817402016-10-28 In vitro maturation of immature rat oocytes under simple culture conditions and subsequent developmental ability TAKETSURU, Hiroaki KANEKO, Takehito J Reprod Dev Original Article Rat oocytes can be produced artificially by superovulation. Because some strains show low sensitivity to superovulation treatment, in vitro maturation is an alternative method to produce numerous matured oocytes. Furthermore, establishment of an in vitro maturation system with simple culture conditions is cost effective and leads to easy handling of oocytes. This study examined developmental ability of rat germinal vesicle (GV) oocytes maturing in vitro under simple culture conditions. Significantly different numbers of ovulated oocytes reached the second metaphase of meiosis (MII) among Jcl:Wistar (17.0), F344/Stm (31.0), and BN/SsNSlc (2.2) rats in whom superovulation was induced by pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin. However, similar numbers of GV oocytes were obtained from ovaries of PMSG-injected Wistar (27.7), F344 (34.7), and BN (24.7) rats. These GV oocytes were cultured in vitro in HTF, αMEM, and a 1:1 HTF + αMEM or TYH + αMEM mixture. High proportions of Wistar and F344 oocytes that matured to MII in αMEM were parthenogenetically activated by strontium chloride treatment (78% and 74%, respectively). Additionally, 10% of matured oocytes of both strains developed into offspring after intracytoplasmic sperm injection and embryo transfer to foster mothers. Although BN oocytes cultured in αMEM could be parthenogenetically activated and developed into offspring, the success rate was lower than that for Wistar and F344 oocytes. This study demonstrated that numerous GV oocytes were produced in rat ovaries by PMSG injection. This simple in vitro maturation system of immature oocytes could be further developed to maintain valuable rat strains experiencing reproductive difficulties. The Society for Reproduction and Development 2016-07-02 2016-10 /pmc/articles/PMC5081740/ /pubmed/27375268 http://dx.doi.org/10.1262/jrd.2016-057 Text en ©2016 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original Article
TAKETSURU, Hiroaki
KANEKO, Takehito
In vitro maturation of immature rat oocytes under simple culture conditions and subsequent developmental ability
title In vitro maturation of immature rat oocytes under simple culture conditions and subsequent developmental ability
title_full In vitro maturation of immature rat oocytes under simple culture conditions and subsequent developmental ability
title_fullStr In vitro maturation of immature rat oocytes under simple culture conditions and subsequent developmental ability
title_full_unstemmed In vitro maturation of immature rat oocytes under simple culture conditions and subsequent developmental ability
title_short In vitro maturation of immature rat oocytes under simple culture conditions and subsequent developmental ability
title_sort in vitro maturation of immature rat oocytes under simple culture conditions and subsequent developmental ability
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081740/
https://www.ncbi.nlm.nih.gov/pubmed/27375268
http://dx.doi.org/10.1262/jrd.2016-057
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