Cargando…

Lactobacillus fermentum ATCC 23271 Displays In vitro Inhibitory Activities against Candida spp.

Lactobacilli are involved in the microbial homeostasis in the female genital tract. Due to the high prevalence of many bacterial diseases of the female genital tract and the resistance of microorganisms to various antimicrobial agents, alternative means to control these infections are necessary. Thu...

Descripción completa

Detalles Bibliográficos
Autores principales: do Carmo, Monique S., Noronha, Francisca M. F., Arruda, Mariana O., Costa, Ênnio P. da Silva, Bomfim, Maria R. Q., Monteiro, Andrea S., Ferro, Thiago A. F., Fernandes, Elizabeth S., Girón, Jorge A., Monteiro-Neto, Valério
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082230/
https://www.ncbi.nlm.nih.gov/pubmed/27833605
http://dx.doi.org/10.3389/fmicb.2016.01722
_version_ 1782463017631350784
author do Carmo, Monique S.
Noronha, Francisca M. F.
Arruda, Mariana O.
Costa, Ênnio P. da Silva
Bomfim, Maria R. Q.
Monteiro, Andrea S.
Ferro, Thiago A. F.
Fernandes, Elizabeth S.
Girón, Jorge A.
Monteiro-Neto, Valério
author_facet do Carmo, Monique S.
Noronha, Francisca M. F.
Arruda, Mariana O.
Costa, Ênnio P. da Silva
Bomfim, Maria R. Q.
Monteiro, Andrea S.
Ferro, Thiago A. F.
Fernandes, Elizabeth S.
Girón, Jorge A.
Monteiro-Neto, Valério
author_sort do Carmo, Monique S.
collection PubMed
description Lactobacilli are involved in the microbial homeostasis in the female genital tract. Due to the high prevalence of many bacterial diseases of the female genital tract and the resistance of microorganisms to various antimicrobial agents, alternative means to control these infections are necessary. Thus, this study aimed to evaluate the probiotic properties of well-characterized Lactobacillus species, including L. acidophilus (ATCC 4356), L. brevis (ATCC 367), L. delbrueckii ssp. delbrueckii (ATCC 9645), L. fermentum (ATCC 23271), L. paracasei (ATCC 335), L. plantarum (ATCC 8014), and L. rhamnosus (ATCC 9595), against Candida albicans (ATCC 18804), Neisseria gonorrhoeae (ATCC 9826), and Streptococcus agalactiae (ATCC 13813). The probiotic potential was investigated by using the following criteria: (i) adhesion to host epithelial cells and mucus, (ii) biofilm formation, (iii) co-aggregation with bacterial pathogens, (iv) inhibition of pathogen adhesion to mucus and HeLa cells, and (v) antimicrobial activity. Tested lactobacilli adhered to mucin, co-aggregated with all genital microorganisms, and displayed antimicrobial activity. With the exception of L. acidophilus and L. paracasei, they adhered to HeLa cells. However, only L. fermentum produced a moderate biofilm and a higher level of co-aggregation and mucin binding. The displacement assay demonstrated that all Lactobacillus strains inhibit C. albicans binding to mucin (p < 0.001), likely due to the production of substances with antimicrobial activity. Clinical isolates belonging to the most common Candida species associated to vaginal candidiasis were inhibited by L. fermentum. Collectively, our data suggest that L. fermentum ATCC 23271 is a potential probiotic candidate, particularly to complement candidiasis treatment, since presented with the best probiotic profile in comparison with the other tested lactobacilli strains.
format Online
Article
Text
id pubmed-5082230
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-50822302016-11-10 Lactobacillus fermentum ATCC 23271 Displays In vitro Inhibitory Activities against Candida spp. do Carmo, Monique S. Noronha, Francisca M. F. Arruda, Mariana O. Costa, Ênnio P. da Silva Bomfim, Maria R. Q. Monteiro, Andrea S. Ferro, Thiago A. F. Fernandes, Elizabeth S. Girón, Jorge A. Monteiro-Neto, Valério Front Microbiol Microbiology Lactobacilli are involved in the microbial homeostasis in the female genital tract. Due to the high prevalence of many bacterial diseases of the female genital tract and the resistance of microorganisms to various antimicrobial agents, alternative means to control these infections are necessary. Thus, this study aimed to evaluate the probiotic properties of well-characterized Lactobacillus species, including L. acidophilus (ATCC 4356), L. brevis (ATCC 367), L. delbrueckii ssp. delbrueckii (ATCC 9645), L. fermentum (ATCC 23271), L. paracasei (ATCC 335), L. plantarum (ATCC 8014), and L. rhamnosus (ATCC 9595), against Candida albicans (ATCC 18804), Neisseria gonorrhoeae (ATCC 9826), and Streptococcus agalactiae (ATCC 13813). The probiotic potential was investigated by using the following criteria: (i) adhesion to host epithelial cells and mucus, (ii) biofilm formation, (iii) co-aggregation with bacterial pathogens, (iv) inhibition of pathogen adhesion to mucus and HeLa cells, and (v) antimicrobial activity. Tested lactobacilli adhered to mucin, co-aggregated with all genital microorganisms, and displayed antimicrobial activity. With the exception of L. acidophilus and L. paracasei, they adhered to HeLa cells. However, only L. fermentum produced a moderate biofilm and a higher level of co-aggregation and mucin binding. The displacement assay demonstrated that all Lactobacillus strains inhibit C. albicans binding to mucin (p < 0.001), likely due to the production of substances with antimicrobial activity. Clinical isolates belonging to the most common Candida species associated to vaginal candidiasis were inhibited by L. fermentum. Collectively, our data suggest that L. fermentum ATCC 23271 is a potential probiotic candidate, particularly to complement candidiasis treatment, since presented with the best probiotic profile in comparison with the other tested lactobacilli strains. Frontiers Media S.A. 2016-10-27 /pmc/articles/PMC5082230/ /pubmed/27833605 http://dx.doi.org/10.3389/fmicb.2016.01722 Text en Copyright © 2016 Carmo, Noronha, Arruda, Costa, Bomfim, Monteiro, Ferro, Fernandes, Girón and Monteiro-Neto. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
do Carmo, Monique S.
Noronha, Francisca M. F.
Arruda, Mariana O.
Costa, Ênnio P. da Silva
Bomfim, Maria R. Q.
Monteiro, Andrea S.
Ferro, Thiago A. F.
Fernandes, Elizabeth S.
Girón, Jorge A.
Monteiro-Neto, Valério
Lactobacillus fermentum ATCC 23271 Displays In vitro Inhibitory Activities against Candida spp.
title Lactobacillus fermentum ATCC 23271 Displays In vitro Inhibitory Activities against Candida spp.
title_full Lactobacillus fermentum ATCC 23271 Displays In vitro Inhibitory Activities against Candida spp.
title_fullStr Lactobacillus fermentum ATCC 23271 Displays In vitro Inhibitory Activities against Candida spp.
title_full_unstemmed Lactobacillus fermentum ATCC 23271 Displays In vitro Inhibitory Activities against Candida spp.
title_short Lactobacillus fermentum ATCC 23271 Displays In vitro Inhibitory Activities against Candida spp.
title_sort lactobacillus fermentum atcc 23271 displays in vitro inhibitory activities against candida spp.
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082230/
https://www.ncbi.nlm.nih.gov/pubmed/27833605
http://dx.doi.org/10.3389/fmicb.2016.01722
work_keys_str_mv AT docarmomoniques lactobacillusfermentumatcc23271displaysinvitroinhibitoryactivitiesagainstcandidaspp
AT noronhafranciscamf lactobacillusfermentumatcc23271displaysinvitroinhibitoryactivitiesagainstcandidaspp
AT arrudamarianao lactobacillusfermentumatcc23271displaysinvitroinhibitoryactivitiesagainstcandidaspp
AT costaenniopdasilva lactobacillusfermentumatcc23271displaysinvitroinhibitoryactivitiesagainstcandidaspp
AT bomfimmariarq lactobacillusfermentumatcc23271displaysinvitroinhibitoryactivitiesagainstcandidaspp
AT monteiroandreas lactobacillusfermentumatcc23271displaysinvitroinhibitoryactivitiesagainstcandidaspp
AT ferrothiagoaf lactobacillusfermentumatcc23271displaysinvitroinhibitoryactivitiesagainstcandidaspp
AT fernandeselizabeths lactobacillusfermentumatcc23271displaysinvitroinhibitoryactivitiesagainstcandidaspp
AT gironjorgea lactobacillusfermentumatcc23271displaysinvitroinhibitoryactivitiesagainstcandidaspp
AT monteironetovalerio lactobacillusfermentumatcc23271displaysinvitroinhibitoryactivitiesagainstcandidaspp