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Oral supplementation of healthy adults with 2′-O-fucosyllactose and lacto-N-neotetraose is well tolerated and shifts the intestinal microbiota
The gut microbiota has been established as an important player influencing many aspects of human physiology. Breast milk, the first diet for an infant, contains human milk oligosaccharides (HMO) that shape the infant’s gut microbiota by selectively stimulating the growth of specific bacteria, especi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cambridge University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082288/ https://www.ncbi.nlm.nih.gov/pubmed/27719686 http://dx.doi.org/10.1017/S0007114516003354 |
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author | Elison, Emma Vigsnaes, Louise K. Rindom Krogsgaard, Laura Rasmussen, Julie Sørensen, Nikolaj McConnell, Bruce Hennet, Thierry Sommer, Morten O. A. Bytzer, Peter |
author_facet | Elison, Emma Vigsnaes, Louise K. Rindom Krogsgaard, Laura Rasmussen, Julie Sørensen, Nikolaj McConnell, Bruce Hennet, Thierry Sommer, Morten O. A. Bytzer, Peter |
author_sort | Elison, Emma |
collection | PubMed |
description | The gut microbiota has been established as an important player influencing many aspects of human physiology. Breast milk, the first diet for an infant, contains human milk oligosaccharides (HMO) that shape the infant’s gut microbiota by selectively stimulating the growth of specific bacteria, especially bifidobacteria. In addition to their bifidogenic activity, the ability of HMO to modulate immune function and the gut barrier makes them prime candidates to restore a beneficial microbiota in dysbiotic adults and provide health benefits. We conducted a parallel, double-blind, randomised, placebo-controlled, HMO-supplementation study in 100 healthy, adult volunteers, consuming chemically produced 2′-O-fucosyllactose (2′FL) and/or lacto-N-neotetraose (LNnT) at various daily doses and mixes or placebo for 2 weeks. All participants completed the study without premature discontinuation. Supplementation of 2′FL and LNnT at daily doses up to 20 g was shown to be safe and well tolerated, as assessed using the gastrointestinal symptoms rating scale. 16S rRNA sequencing analysis showed that HMO supplementation specifically modified the adult gut microbiota with the primary impact being substantial increases in relative abundance of Actinobacteria and Bifidobacterium in particular and a reduction in relative abundance of Firmicutes and Proteobacteria. This study provides the first set of data on safety, tolerance and impact of HMO on the adult gut microbiota. Collectively, the results from this study show that supplementing the diet with HMO is a valuable strategy to shape the human gut microbiota and specifically promote the growth of beneficial bifidobacteria. |
format | Online Article Text |
id | pubmed-5082288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Cambridge University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-50822882016-11-07 Oral supplementation of healthy adults with 2′-O-fucosyllactose and lacto-N-neotetraose is well tolerated and shifts the intestinal microbiota Elison, Emma Vigsnaes, Louise K. Rindom Krogsgaard, Laura Rasmussen, Julie Sørensen, Nikolaj McConnell, Bruce Hennet, Thierry Sommer, Morten O. A. Bytzer, Peter Br J Nutr Full Papers The gut microbiota has been established as an important player influencing many aspects of human physiology. Breast milk, the first diet for an infant, contains human milk oligosaccharides (HMO) that shape the infant’s gut microbiota by selectively stimulating the growth of specific bacteria, especially bifidobacteria. In addition to their bifidogenic activity, the ability of HMO to modulate immune function and the gut barrier makes them prime candidates to restore a beneficial microbiota in dysbiotic adults and provide health benefits. We conducted a parallel, double-blind, randomised, placebo-controlled, HMO-supplementation study in 100 healthy, adult volunteers, consuming chemically produced 2′-O-fucosyllactose (2′FL) and/or lacto-N-neotetraose (LNnT) at various daily doses and mixes or placebo for 2 weeks. All participants completed the study without premature discontinuation. Supplementation of 2′FL and LNnT at daily doses up to 20 g was shown to be safe and well tolerated, as assessed using the gastrointestinal symptoms rating scale. 16S rRNA sequencing analysis showed that HMO supplementation specifically modified the adult gut microbiota with the primary impact being substantial increases in relative abundance of Actinobacteria and Bifidobacterium in particular and a reduction in relative abundance of Firmicutes and Proteobacteria. This study provides the first set of data on safety, tolerance and impact of HMO on the adult gut microbiota. Collectively, the results from this study show that supplementing the diet with HMO is a valuable strategy to shape the human gut microbiota and specifically promote the growth of beneficial bifidobacteria. Cambridge University Press 2016-10-10 2016-10-28 /pmc/articles/PMC5082288/ /pubmed/27719686 http://dx.doi.org/10.1017/S0007114516003354 Text en © The Authors 2016 http://creativecommons.org/licenses/by/4.0/ This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Papers Elison, Emma Vigsnaes, Louise K. Rindom Krogsgaard, Laura Rasmussen, Julie Sørensen, Nikolaj McConnell, Bruce Hennet, Thierry Sommer, Morten O. A. Bytzer, Peter Oral supplementation of healthy adults with 2′-O-fucosyllactose and lacto-N-neotetraose is well tolerated and shifts the intestinal microbiota |
title | Oral supplementation of healthy adults with
2′-O-fucosyllactose and lacto-N-neotetraose is well
tolerated and shifts the intestinal microbiota |
title_full | Oral supplementation of healthy adults with
2′-O-fucosyllactose and lacto-N-neotetraose is well
tolerated and shifts the intestinal microbiota |
title_fullStr | Oral supplementation of healthy adults with
2′-O-fucosyllactose and lacto-N-neotetraose is well
tolerated and shifts the intestinal microbiota |
title_full_unstemmed | Oral supplementation of healthy adults with
2′-O-fucosyllactose and lacto-N-neotetraose is well
tolerated and shifts the intestinal microbiota |
title_short | Oral supplementation of healthy adults with
2′-O-fucosyllactose and lacto-N-neotetraose is well
tolerated and shifts the intestinal microbiota |
title_sort | oral supplementation of healthy adults with
2′-o-fucosyllactose and lacto-n-neotetraose is well
tolerated and shifts the intestinal microbiota |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082288/ https://www.ncbi.nlm.nih.gov/pubmed/27719686 http://dx.doi.org/10.1017/S0007114516003354 |
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