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Identification of a Pro-Angiogenic Potential and Cellular Uptake Mechanism of a LMW Highly Sulfated Fraction of Fucoidan from Ascophyllum nodosum

Herein we investigate the structure/function relationships of fucoidans from Ascophyllum nodosum to analyze their pro-angiogenic effect and cellular uptake in native and glycosaminoglycan-free (GAG-free) human endothelial cells (HUVECs). Fucoidans are marine sulfated polysaccharides, which act as gl...

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Autores principales: Marinval, Nicolas, Saboural, Pierre, Haddad, Oualid, Maire, Murielle, Bassand, Kevin, Geinguenaud, Frederic, Djaker, Nadia, Ben Akrout, Khadija, Lamy de la Chapelle, Marc, Robert, Romain, Oudar, Olivier, Guyot, Erwan, Laguillier-Morizot, Christelle, Sutton, Angela, Chauvierre, Cedric, Chaubet, Frederic, Charnaux, Nathalie, Hlawaty, Hanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082333/
https://www.ncbi.nlm.nih.gov/pubmed/27763505
http://dx.doi.org/10.3390/md14100185
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author Marinval, Nicolas
Saboural, Pierre
Haddad, Oualid
Maire, Murielle
Bassand, Kevin
Geinguenaud, Frederic
Djaker, Nadia
Ben Akrout, Khadija
Lamy de la Chapelle, Marc
Robert, Romain
Oudar, Olivier
Guyot, Erwan
Laguillier-Morizot, Christelle
Sutton, Angela
Chauvierre, Cedric
Chaubet, Frederic
Charnaux, Nathalie
Hlawaty, Hanna
author_facet Marinval, Nicolas
Saboural, Pierre
Haddad, Oualid
Maire, Murielle
Bassand, Kevin
Geinguenaud, Frederic
Djaker, Nadia
Ben Akrout, Khadija
Lamy de la Chapelle, Marc
Robert, Romain
Oudar, Olivier
Guyot, Erwan
Laguillier-Morizot, Christelle
Sutton, Angela
Chauvierre, Cedric
Chaubet, Frederic
Charnaux, Nathalie
Hlawaty, Hanna
author_sort Marinval, Nicolas
collection PubMed
description Herein we investigate the structure/function relationships of fucoidans from Ascophyllum nodosum to analyze their pro-angiogenic effect and cellular uptake in native and glycosaminoglycan-free (GAG-free) human endothelial cells (HUVECs). Fucoidans are marine sulfated polysaccharides, which act as glycosaminoglycans mimetics. We hypothesized that the size and sulfation rate of fucoidans influence their ability to induce pro-angiogenic processes independently of GAGs. We collected two fractions of fucoidans, Low and Medium Molecular Weight Fucoidan (LMWF and MMWF, respectively) by size exclusion chromatography and characterized their composition (sulfate, fucose and uronic acid) by colorimetric measurement and Raman and FT-IR spectroscopy. The high affinities of fractionated fucoidans to heparin binding proteins were confirmed by Surface Plasmon Resonance. We evidenced that LMWF has a higher pro-angiogenic (2D-angiogenesis on Matrigel) and pro-migratory (Boyden chamber) potential on HUVECs, compared to MMWF. Interestingly, in a GAG-free HUVECs model, LMWF kept a pro-angiogenic potential. Finally, to evaluate the association of LMWF-induced biological effects and its cellular uptake, we analyzed by confocal microscopy the GAGs involvement in the internalization of a fluorescent LMWF. The fluorescent LMWF was mainly internalized through HUVEC clathrin-dependent endocytosis in which GAGs were partially involved. In conclusion, a better characterization of the relationships between the fucoidan structure and its pro-angiogenic potential in GAG-free endothelial cells was required to identify an adapted fucoidan to enhance vascular repair in ischemia.
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spelling pubmed-50823332016-10-28 Identification of a Pro-Angiogenic Potential and Cellular Uptake Mechanism of a LMW Highly Sulfated Fraction of Fucoidan from Ascophyllum nodosum Marinval, Nicolas Saboural, Pierre Haddad, Oualid Maire, Murielle Bassand, Kevin Geinguenaud, Frederic Djaker, Nadia Ben Akrout, Khadija Lamy de la Chapelle, Marc Robert, Romain Oudar, Olivier Guyot, Erwan Laguillier-Morizot, Christelle Sutton, Angela Chauvierre, Cedric Chaubet, Frederic Charnaux, Nathalie Hlawaty, Hanna Mar Drugs Article Herein we investigate the structure/function relationships of fucoidans from Ascophyllum nodosum to analyze their pro-angiogenic effect and cellular uptake in native and glycosaminoglycan-free (GAG-free) human endothelial cells (HUVECs). Fucoidans are marine sulfated polysaccharides, which act as glycosaminoglycans mimetics. We hypothesized that the size and sulfation rate of fucoidans influence their ability to induce pro-angiogenic processes independently of GAGs. We collected two fractions of fucoidans, Low and Medium Molecular Weight Fucoidan (LMWF and MMWF, respectively) by size exclusion chromatography and characterized their composition (sulfate, fucose and uronic acid) by colorimetric measurement and Raman and FT-IR spectroscopy. The high affinities of fractionated fucoidans to heparin binding proteins were confirmed by Surface Plasmon Resonance. We evidenced that LMWF has a higher pro-angiogenic (2D-angiogenesis on Matrigel) and pro-migratory (Boyden chamber) potential on HUVECs, compared to MMWF. Interestingly, in a GAG-free HUVECs model, LMWF kept a pro-angiogenic potential. Finally, to evaluate the association of LMWF-induced biological effects and its cellular uptake, we analyzed by confocal microscopy the GAGs involvement in the internalization of a fluorescent LMWF. The fluorescent LMWF was mainly internalized through HUVEC clathrin-dependent endocytosis in which GAGs were partially involved. In conclusion, a better characterization of the relationships between the fucoidan structure and its pro-angiogenic potential in GAG-free endothelial cells was required to identify an adapted fucoidan to enhance vascular repair in ischemia. MDPI 2016-10-17 /pmc/articles/PMC5082333/ /pubmed/27763505 http://dx.doi.org/10.3390/md14100185 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marinval, Nicolas
Saboural, Pierre
Haddad, Oualid
Maire, Murielle
Bassand, Kevin
Geinguenaud, Frederic
Djaker, Nadia
Ben Akrout, Khadija
Lamy de la Chapelle, Marc
Robert, Romain
Oudar, Olivier
Guyot, Erwan
Laguillier-Morizot, Christelle
Sutton, Angela
Chauvierre, Cedric
Chaubet, Frederic
Charnaux, Nathalie
Hlawaty, Hanna
Identification of a Pro-Angiogenic Potential and Cellular Uptake Mechanism of a LMW Highly Sulfated Fraction of Fucoidan from Ascophyllum nodosum
title Identification of a Pro-Angiogenic Potential and Cellular Uptake Mechanism of a LMW Highly Sulfated Fraction of Fucoidan from Ascophyllum nodosum
title_full Identification of a Pro-Angiogenic Potential and Cellular Uptake Mechanism of a LMW Highly Sulfated Fraction of Fucoidan from Ascophyllum nodosum
title_fullStr Identification of a Pro-Angiogenic Potential and Cellular Uptake Mechanism of a LMW Highly Sulfated Fraction of Fucoidan from Ascophyllum nodosum
title_full_unstemmed Identification of a Pro-Angiogenic Potential and Cellular Uptake Mechanism of a LMW Highly Sulfated Fraction of Fucoidan from Ascophyllum nodosum
title_short Identification of a Pro-Angiogenic Potential and Cellular Uptake Mechanism of a LMW Highly Sulfated Fraction of Fucoidan from Ascophyllum nodosum
title_sort identification of a pro-angiogenic potential and cellular uptake mechanism of a lmw highly sulfated fraction of fucoidan from ascophyllum nodosum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082333/
https://www.ncbi.nlm.nih.gov/pubmed/27763505
http://dx.doi.org/10.3390/md14100185
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