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Different Culture Metabolites of the Red Sea Fungus Fusarium equiseti Optimize the Inhibition of Hepatitis C Virus NS3/4A Protease (HCV PR)

The endophytic fungus Fusarium equiseti was isolated from the brown alga Padina pavonica, collected from the Red Sea. The fungus was identified by its morphology and 18S rDNA. Cultivation of this fungal strain in biomalt-peptone medium led to isolation of 12 known metabolites of diketopeprazines and...

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Autores principales: Hawas, Usama W., Al-Farawati, Radwan, Abou El-Kassem, Lamia T., Turki, Adnan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082338/
https://www.ncbi.nlm.nih.gov/pubmed/27775589
http://dx.doi.org/10.3390/md14100190
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author Hawas, Usama W.
Al-Farawati, Radwan
Abou El-Kassem, Lamia T.
Turki, Adnan J.
author_facet Hawas, Usama W.
Al-Farawati, Radwan
Abou El-Kassem, Lamia T.
Turki, Adnan J.
author_sort Hawas, Usama W.
collection PubMed
description The endophytic fungus Fusarium equiseti was isolated from the brown alga Padina pavonica, collected from the Red Sea. The fungus was identified by its morphology and 18S rDNA. Cultivation of this fungal strain in biomalt-peptone medium led to isolation of 12 known metabolites of diketopeprazines and anthraquinones. The organic extract and isolated compounds were screened for their inhibition of hepatitis C virus NS3/4A protease (HCV PR). As a result, the fungal metabolites showed inhibition of HCV protease (IC(50) from 19 to 77 μM), and the fungus was subjected to culture on Czapek’s (Cz) media, with a yield of nine metabolites with potent HCV protease inhibition ranging from IC(50) 10 to 37 μM. The Cz culture extract exhibited high-level inhibition of HCV protease (IC(50) 27.6 μg/mL) compared to the biomalt culture extract (IC(50) 56 μg/mL), and the most potent HCV PR isolated compound (Griseoxanthone C, IC(50) 19.8 μM) from the bio-malt culture extract showed less of an inhibitory effect compared to isolated ω-hydroxyemodin (IC(50) 10.7 μM) from the optimized Cz culture extract. Both HCV PR active inhibitors ω-hydroxyemodin and griseoxanthone C were considered as the lowest selective safe constituents against Trypsin inhibitory effect with IC(50) 48.5 and 51.3 μM, respectively.
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spelling pubmed-50823382016-10-28 Different Culture Metabolites of the Red Sea Fungus Fusarium equiseti Optimize the Inhibition of Hepatitis C Virus NS3/4A Protease (HCV PR) Hawas, Usama W. Al-Farawati, Radwan Abou El-Kassem, Lamia T. Turki, Adnan J. Mar Drugs Article The endophytic fungus Fusarium equiseti was isolated from the brown alga Padina pavonica, collected from the Red Sea. The fungus was identified by its morphology and 18S rDNA. Cultivation of this fungal strain in biomalt-peptone medium led to isolation of 12 known metabolites of diketopeprazines and anthraquinones. The organic extract and isolated compounds were screened for their inhibition of hepatitis C virus NS3/4A protease (HCV PR). As a result, the fungal metabolites showed inhibition of HCV protease (IC(50) from 19 to 77 μM), and the fungus was subjected to culture on Czapek’s (Cz) media, with a yield of nine metabolites with potent HCV protease inhibition ranging from IC(50) 10 to 37 μM. The Cz culture extract exhibited high-level inhibition of HCV protease (IC(50) 27.6 μg/mL) compared to the biomalt culture extract (IC(50) 56 μg/mL), and the most potent HCV PR isolated compound (Griseoxanthone C, IC(50) 19.8 μM) from the bio-malt culture extract showed less of an inhibitory effect compared to isolated ω-hydroxyemodin (IC(50) 10.7 μM) from the optimized Cz culture extract. Both HCV PR active inhibitors ω-hydroxyemodin and griseoxanthone C were considered as the lowest selective safe constituents against Trypsin inhibitory effect with IC(50) 48.5 and 51.3 μM, respectively. MDPI 2016-10-20 /pmc/articles/PMC5082338/ /pubmed/27775589 http://dx.doi.org/10.3390/md14100190 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hawas, Usama W.
Al-Farawati, Radwan
Abou El-Kassem, Lamia T.
Turki, Adnan J.
Different Culture Metabolites of the Red Sea Fungus Fusarium equiseti Optimize the Inhibition of Hepatitis C Virus NS3/4A Protease (HCV PR)
title Different Culture Metabolites of the Red Sea Fungus Fusarium equiseti Optimize the Inhibition of Hepatitis C Virus NS3/4A Protease (HCV PR)
title_full Different Culture Metabolites of the Red Sea Fungus Fusarium equiseti Optimize the Inhibition of Hepatitis C Virus NS3/4A Protease (HCV PR)
title_fullStr Different Culture Metabolites of the Red Sea Fungus Fusarium equiseti Optimize the Inhibition of Hepatitis C Virus NS3/4A Protease (HCV PR)
title_full_unstemmed Different Culture Metabolites of the Red Sea Fungus Fusarium equiseti Optimize the Inhibition of Hepatitis C Virus NS3/4A Protease (HCV PR)
title_short Different Culture Metabolites of the Red Sea Fungus Fusarium equiseti Optimize the Inhibition of Hepatitis C Virus NS3/4A Protease (HCV PR)
title_sort different culture metabolites of the red sea fungus fusarium equiseti optimize the inhibition of hepatitis c virus ns3/4a protease (hcv pr)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082338/
https://www.ncbi.nlm.nih.gov/pubmed/27775589
http://dx.doi.org/10.3390/md14100190
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