Nuclear incorporation of iron during the eukaryotic cell cycle

Scanning X-ray fluorescence microscopy has been used to probe the distribution of S, P and Fe within cell nuclei. Nuclei, which may have originated at different phases of the cell cycle, are found to show very different levels of Fe present with a strongly inhomogeneous distribution. P and S signals...

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Detalles Bibliográficos
Autores principales: Robinson, Ian, Yang, Yang, Zhang, Fucai, Lynch, Christophe, Yusuf, Mohammed, Cloetens, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2016
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082466/
https://www.ncbi.nlm.nih.gov/pubmed/27787255
http://dx.doi.org/10.1107/S1600577516012807
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author Robinson, Ian
Yang, Yang
Zhang, Fucai
Lynch, Christophe
Yusuf, Mohammed
Cloetens, Peter
author_facet Robinson, Ian
Yang, Yang
Zhang, Fucai
Lynch, Christophe
Yusuf, Mohammed
Cloetens, Peter
author_sort Robinson, Ian
collection PubMed
description Scanning X-ray fluorescence microscopy has been used to probe the distribution of S, P and Fe within cell nuclei. Nuclei, which may have originated at different phases of the cell cycle, are found to show very different levels of Fe present with a strongly inhomogeneous distribution. P and S signals, presumably from DNA and associated nucleosomes, are high and relatively uniform across all the nuclei; these agree with X-ray phase contrast projection microscopy images of the same samples. Possible reasons for the Fe incorporation are discussed.
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spelling pubmed-50824662016-11-11 Nuclear incorporation of iron during the eukaryotic cell cycle Robinson, Ian Yang, Yang Zhang, Fucai Lynch, Christophe Yusuf, Mohammed Cloetens, Peter J Synchrotron Radiat Research Papers Scanning X-ray fluorescence microscopy has been used to probe the distribution of S, P and Fe within cell nuclei. Nuclei, which may have originated at different phases of the cell cycle, are found to show very different levels of Fe present with a strongly inhomogeneous distribution. P and S signals, presumably from DNA and associated nucleosomes, are high and relatively uniform across all the nuclei; these agree with X-ray phase contrast projection microscopy images of the same samples. Possible reasons for the Fe incorporation are discussed. International Union of Crystallography 2016-10-18 /pmc/articles/PMC5082466/ /pubmed/27787255 http://dx.doi.org/10.1107/S1600577516012807 Text en © Ian Robinson et al. 2016 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Robinson, Ian
Yang, Yang
Zhang, Fucai
Lynch, Christophe
Yusuf, Mohammed
Cloetens, Peter
Nuclear incorporation of iron during the eukaryotic cell cycle
title Nuclear incorporation of iron during the eukaryotic cell cycle
title_full Nuclear incorporation of iron during the eukaryotic cell cycle
title_fullStr Nuclear incorporation of iron during the eukaryotic cell cycle
title_full_unstemmed Nuclear incorporation of iron during the eukaryotic cell cycle
title_short Nuclear incorporation of iron during the eukaryotic cell cycle
title_sort nuclear incorporation of iron during the eukaryotic cell cycle
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082466/
https://www.ncbi.nlm.nih.gov/pubmed/27787255
http://dx.doi.org/10.1107/S1600577516012807
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