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Detection and imaging of quorum sensing in Pseudomonas aeruginosa biofilm communities by surface-enhanced resonance Raman scattering

Most bacteria in nature exist as biofilms, which support intercellular signaling processes such as quorum sensing (QS), a cell-to-cell communication mechanism that allows bacteria to monitor and respond to cell density and changes in the environment. Because QS and biofilms are involved in the abili...

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Autores principales: Bodelón, Gustavo, Montes-García, Verónica, López-Puente, Vanesa, Hill, Eric H., Hamon, Cyrille, Sanz-Ortiz, Marta N., Rodal-Cedeira, Sergio, Costas, Celina, Celiksoy, Sirin, Pérez-Juste, Ignacio, Scarabelli, Leonardo, Porta, Andrea La, Pérez-Juste, Jorge, Pastoriza-Santos, Isabel, Liz-Marzán, Luis M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082732/
https://www.ncbi.nlm.nih.gov/pubmed/27500808
http://dx.doi.org/10.1038/nmat4720
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author Bodelón, Gustavo
Montes-García, Verónica
López-Puente, Vanesa
Hill, Eric H.
Hamon, Cyrille
Sanz-Ortiz, Marta N.
Rodal-Cedeira, Sergio
Costas, Celina
Celiksoy, Sirin
Pérez-Juste, Ignacio
Scarabelli, Leonardo
Porta, Andrea La
Pérez-Juste, Jorge
Pastoriza-Santos, Isabel
Liz-Marzán, Luis M.
author_facet Bodelón, Gustavo
Montes-García, Verónica
López-Puente, Vanesa
Hill, Eric H.
Hamon, Cyrille
Sanz-Ortiz, Marta N.
Rodal-Cedeira, Sergio
Costas, Celina
Celiksoy, Sirin
Pérez-Juste, Ignacio
Scarabelli, Leonardo
Porta, Andrea La
Pérez-Juste, Jorge
Pastoriza-Santos, Isabel
Liz-Marzán, Luis M.
author_sort Bodelón, Gustavo
collection PubMed
description Most bacteria in nature exist as biofilms, which support intercellular signaling processes such as quorum sensing (QS), a cell-to-cell communication mechanism that allows bacteria to monitor and respond to cell density and changes in the environment. Because QS and biofilms are involved in the ability of bacteria to cause disease, there is a need for the development of methods for the non-invasive analysis of QS in natural bacterial populations. Here, by using surface-enhanced resonance Raman scattering spectroscopy, we report rationally designed nanostructured plasmonic substrates for the in-situ, label-free detection of a QS signaling metabolite in growing Pseudomonas aeruginosa biofilms and microcolonies. The in situ, non-invasive plasmonic imaging of QS in biofilms provides a powerful analytical approach for studying intercellular communication on the basis of secreted molecules as signals.
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spelling pubmed-50827322017-02-08 Detection and imaging of quorum sensing in Pseudomonas aeruginosa biofilm communities by surface-enhanced resonance Raman scattering Bodelón, Gustavo Montes-García, Verónica López-Puente, Vanesa Hill, Eric H. Hamon, Cyrille Sanz-Ortiz, Marta N. Rodal-Cedeira, Sergio Costas, Celina Celiksoy, Sirin Pérez-Juste, Ignacio Scarabelli, Leonardo Porta, Andrea La Pérez-Juste, Jorge Pastoriza-Santos, Isabel Liz-Marzán, Luis M. Nat Mater Article Most bacteria in nature exist as biofilms, which support intercellular signaling processes such as quorum sensing (QS), a cell-to-cell communication mechanism that allows bacteria to monitor and respond to cell density and changes in the environment. Because QS and biofilms are involved in the ability of bacteria to cause disease, there is a need for the development of methods for the non-invasive analysis of QS in natural bacterial populations. Here, by using surface-enhanced resonance Raman scattering spectroscopy, we report rationally designed nanostructured plasmonic substrates for the in-situ, label-free detection of a QS signaling metabolite in growing Pseudomonas aeruginosa biofilms and microcolonies. The in situ, non-invasive plasmonic imaging of QS in biofilms provides a powerful analytical approach for studying intercellular communication on the basis of secreted molecules as signals. 2016-08-08 2016-11 /pmc/articles/PMC5082732/ /pubmed/27500808 http://dx.doi.org/10.1038/nmat4720 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Bodelón, Gustavo
Montes-García, Verónica
López-Puente, Vanesa
Hill, Eric H.
Hamon, Cyrille
Sanz-Ortiz, Marta N.
Rodal-Cedeira, Sergio
Costas, Celina
Celiksoy, Sirin
Pérez-Juste, Ignacio
Scarabelli, Leonardo
Porta, Andrea La
Pérez-Juste, Jorge
Pastoriza-Santos, Isabel
Liz-Marzán, Luis M.
Detection and imaging of quorum sensing in Pseudomonas aeruginosa biofilm communities by surface-enhanced resonance Raman scattering
title Detection and imaging of quorum sensing in Pseudomonas aeruginosa biofilm communities by surface-enhanced resonance Raman scattering
title_full Detection and imaging of quorum sensing in Pseudomonas aeruginosa biofilm communities by surface-enhanced resonance Raman scattering
title_fullStr Detection and imaging of quorum sensing in Pseudomonas aeruginosa biofilm communities by surface-enhanced resonance Raman scattering
title_full_unstemmed Detection and imaging of quorum sensing in Pseudomonas aeruginosa biofilm communities by surface-enhanced resonance Raman scattering
title_short Detection and imaging of quorum sensing in Pseudomonas aeruginosa biofilm communities by surface-enhanced resonance Raman scattering
title_sort detection and imaging of quorum sensing in pseudomonas aeruginosa biofilm communities by surface-enhanced resonance raman scattering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082732/
https://www.ncbi.nlm.nih.gov/pubmed/27500808
http://dx.doi.org/10.1038/nmat4720
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