Cargando…

A Viral Immunity Chromosome in the Marine Picoeukaryote, Ostreococcus tauri

Micro-algae of the genus Ostreococcus and related species of the order Mamiellales are globally distributed in the photic zone of world's oceans where they contribute to fixation of atmospheric carbon and production of oxygen, besides providing a primary source of nutrition in the food web. The...

Descripción completa

Detalles Bibliográficos
Autores principales: Yau, Sheree, Hemon, Claire, Derelle, Evelyne, Moreau, Hervé, Piganeau, Gwenaël, Grimsley, Nigel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082852/
https://www.ncbi.nlm.nih.gov/pubmed/27788272
http://dx.doi.org/10.1371/journal.ppat.1005965
_version_ 1782463134344151040
author Yau, Sheree
Hemon, Claire
Derelle, Evelyne
Moreau, Hervé
Piganeau, Gwenaël
Grimsley, Nigel
author_facet Yau, Sheree
Hemon, Claire
Derelle, Evelyne
Moreau, Hervé
Piganeau, Gwenaël
Grimsley, Nigel
author_sort Yau, Sheree
collection PubMed
description Micro-algae of the genus Ostreococcus and related species of the order Mamiellales are globally distributed in the photic zone of world's oceans where they contribute to fixation of atmospheric carbon and production of oxygen, besides providing a primary source of nutrition in the food web. Their tiny size, simple cells, ease of culture, compact genomes and susceptibility to the most abundant large DNA viruses in the sea render them attractive as models for integrative marine biology. In culture, spontaneous resistance to viruses occurs frequently. Here, we show that virus-producing resistant cell lines arise in many independent cell lines during lytic infections, but over two years, more and more of these lines stop producing viruses. We observed sweeping over-expression of all genes in more than half of chromosome 19 in resistant lines, and karyotypic analyses showed physical rearrangements of this chromosome. Chromosome 19 has an unusual genetic structure whose equivalent is found in all of the sequenced genomes in this ecologically important group of green algae.
format Online
Article
Text
id pubmed-5082852
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-50828522016-11-04 A Viral Immunity Chromosome in the Marine Picoeukaryote, Ostreococcus tauri Yau, Sheree Hemon, Claire Derelle, Evelyne Moreau, Hervé Piganeau, Gwenaël Grimsley, Nigel PLoS Pathog Research Article Micro-algae of the genus Ostreococcus and related species of the order Mamiellales are globally distributed in the photic zone of world's oceans where they contribute to fixation of atmospheric carbon and production of oxygen, besides providing a primary source of nutrition in the food web. Their tiny size, simple cells, ease of culture, compact genomes and susceptibility to the most abundant large DNA viruses in the sea render them attractive as models for integrative marine biology. In culture, spontaneous resistance to viruses occurs frequently. Here, we show that virus-producing resistant cell lines arise in many independent cell lines during lytic infections, but over two years, more and more of these lines stop producing viruses. We observed sweeping over-expression of all genes in more than half of chromosome 19 in resistant lines, and karyotypic analyses showed physical rearrangements of this chromosome. Chromosome 19 has an unusual genetic structure whose equivalent is found in all of the sequenced genomes in this ecologically important group of green algae. Public Library of Science 2016-10-27 /pmc/articles/PMC5082852/ /pubmed/27788272 http://dx.doi.org/10.1371/journal.ppat.1005965 Text en © 2016 Yau et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yau, Sheree
Hemon, Claire
Derelle, Evelyne
Moreau, Hervé
Piganeau, Gwenaël
Grimsley, Nigel
A Viral Immunity Chromosome in the Marine Picoeukaryote, Ostreococcus tauri
title A Viral Immunity Chromosome in the Marine Picoeukaryote, Ostreococcus tauri
title_full A Viral Immunity Chromosome in the Marine Picoeukaryote, Ostreococcus tauri
title_fullStr A Viral Immunity Chromosome in the Marine Picoeukaryote, Ostreococcus tauri
title_full_unstemmed A Viral Immunity Chromosome in the Marine Picoeukaryote, Ostreococcus tauri
title_short A Viral Immunity Chromosome in the Marine Picoeukaryote, Ostreococcus tauri
title_sort viral immunity chromosome in the marine picoeukaryote, ostreococcus tauri
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082852/
https://www.ncbi.nlm.nih.gov/pubmed/27788272
http://dx.doi.org/10.1371/journal.ppat.1005965
work_keys_str_mv AT yausheree aviralimmunitychromosomeinthemarinepicoeukaryoteostreococcustauri
AT hemonclaire aviralimmunitychromosomeinthemarinepicoeukaryoteostreococcustauri
AT derelleevelyne aviralimmunitychromosomeinthemarinepicoeukaryoteostreococcustauri
AT moreauherve aviralimmunitychromosomeinthemarinepicoeukaryoteostreococcustauri
AT piganeaugwenael aviralimmunitychromosomeinthemarinepicoeukaryoteostreococcustauri
AT grimsleynigel aviralimmunitychromosomeinthemarinepicoeukaryoteostreococcustauri
AT yausheree viralimmunitychromosomeinthemarinepicoeukaryoteostreococcustauri
AT hemonclaire viralimmunitychromosomeinthemarinepicoeukaryoteostreococcustauri
AT derelleevelyne viralimmunitychromosomeinthemarinepicoeukaryoteostreococcustauri
AT moreauherve viralimmunitychromosomeinthemarinepicoeukaryoteostreococcustauri
AT piganeaugwenael viralimmunitychromosomeinthemarinepicoeukaryoteostreococcustauri
AT grimsleynigel viralimmunitychromosomeinthemarinepicoeukaryoteostreococcustauri