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Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans
Temperature sensation by the nervous system is essential for life and proliferation of animals. The molecular-physiological mechanisms underlying temperature signaling have not been fully elucidated. We show here that diverse regulatory machinery underlies temperature sensation through trimeric G-pr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082853/ https://www.ncbi.nlm.nih.gov/pubmed/27788246 http://dx.doi.org/10.1371/journal.pone.0165518 |
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author | Ujisawa, Tomoyo Ohta, Akane Uda-Yagi, Misato Kuhara, Atsushi |
author_facet | Ujisawa, Tomoyo Ohta, Akane Uda-Yagi, Misato Kuhara, Atsushi |
author_sort | Ujisawa, Tomoyo |
collection | PubMed |
description | Temperature sensation by the nervous system is essential for life and proliferation of animals. The molecular-physiological mechanisms underlying temperature signaling have not been fully elucidated. We show here that diverse regulatory machinery underlies temperature sensation through trimeric G-protein signaling in the nematode Caenorhabditis elegans. Molecular-genetic studies demonstrated that cold tolerance is regulated by additive functions of three Gα proteins in a temperature-sensing neuron, ASJ, which is also known to be a light-sensing neuron. Optical recording of calcium concentration in ASJ upon temperature-changes demonstrated that three Gα proteins act in different aspects of temperature signaling. Calcium concentration changes in ASJ upon temperature change were unexpectedly decreased in a mutant defective in phosphodiesterase, which is well known as a negative regulator of calcium increase. Together, these data demonstrate commonalities and differences in the molecular components concerned with light and temperature signaling in a single sensory neuron. |
format | Online Article Text |
id | pubmed-5082853 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-50828532016-11-04 Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans Ujisawa, Tomoyo Ohta, Akane Uda-Yagi, Misato Kuhara, Atsushi PLoS One Research Article Temperature sensation by the nervous system is essential for life and proliferation of animals. The molecular-physiological mechanisms underlying temperature signaling have not been fully elucidated. We show here that diverse regulatory machinery underlies temperature sensation through trimeric G-protein signaling in the nematode Caenorhabditis elegans. Molecular-genetic studies demonstrated that cold tolerance is regulated by additive functions of three Gα proteins in a temperature-sensing neuron, ASJ, which is also known to be a light-sensing neuron. Optical recording of calcium concentration in ASJ upon temperature-changes demonstrated that three Gα proteins act in different aspects of temperature signaling. Calcium concentration changes in ASJ upon temperature change were unexpectedly decreased in a mutant defective in phosphodiesterase, which is well known as a negative regulator of calcium increase. Together, these data demonstrate commonalities and differences in the molecular components concerned with light and temperature signaling in a single sensory neuron. Public Library of Science 2016-10-27 /pmc/articles/PMC5082853/ /pubmed/27788246 http://dx.doi.org/10.1371/journal.pone.0165518 Text en © 2016 Ujisawa et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ujisawa, Tomoyo Ohta, Akane Uda-Yagi, Misato Kuhara, Atsushi Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans |
title | Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans |
title_full | Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans |
title_fullStr | Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans |
title_full_unstemmed | Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans |
title_short | Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans |
title_sort | diverse regulation of temperature sensation by trimeric g-protein signaling in caenorhabditis elegans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082853/ https://www.ncbi.nlm.nih.gov/pubmed/27788246 http://dx.doi.org/10.1371/journal.pone.0165518 |
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