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Native Killer Yeasts as Biocontrol Agents of Postharvest Fungal Diseases in Lemons
Economic losses caused by postharvest diseases represent one of the main problems of the citrus industry worldwide. The major diseases affecting citrus are the "green mold" and "blue mold", caused by Penicillium digitatum and P. italicum, respectively. To control them, synthetic...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5085023/ https://www.ncbi.nlm.nih.gov/pubmed/27792761 http://dx.doi.org/10.1371/journal.pone.0165590 |
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author | Perez, María Florencia Contreras, Luciana Garnica, Nydia Mercedes Fernández-Zenoff, María Verónica Farías, María Eugenia Sepulveda, Milena Ramallo, Jacqueline Dib, Julián Rafael |
author_facet | Perez, María Florencia Contreras, Luciana Garnica, Nydia Mercedes Fernández-Zenoff, María Verónica Farías, María Eugenia Sepulveda, Milena Ramallo, Jacqueline Dib, Julián Rafael |
author_sort | Perez, María Florencia |
collection | PubMed |
description | Economic losses caused by postharvest diseases represent one of the main problems of the citrus industry worldwide. The major diseases affecting citrus are the "green mold" and "blue mold", caused by Penicillium digitatum and P. italicum, respectively. To control them, synthetic fungicides are the most commonly used method. However, often the emergence of resistant strains occurs and their use is becoming more restricted because of toxic effects and environmental pollution they generate, combined with trade barriers to international markets. The aim of this work was to isolate indigenous killer yeasts with antagonistic activity against fungal postharvest diseases in lemons, and to determine their control efficiency in in vitro and in vivo assays. Among 437 yeast isolates, 8.5% show to have a killer phenotype. According to molecular identification, based on the 26S rDNA D1/D2 domain sequences analysis, strains were identified belonging to the genera Saccharomyces, Wickerhamomyces, Kazachstania, Pichia, Candida and Clavispora. Killers were challenged with pathogenic molds and strains that caused the maximum in vitro inhibition of P. digitatum were selected for in vivo assays. Two strains of Pichia and one strain of Wickerhamomyces depicted a significant protection (p <0.05) from decay by P. digitatum in assays using wounded lemons. Thus, the native killer yeasts studied in this work showed to be an effective alternative for the biocontrol of postharvest fungal infections of lemons and could be promising agents for the development of commercial products for the biological control industry. |
format | Online Article Text |
id | pubmed-5085023 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-50850232016-11-04 Native Killer Yeasts as Biocontrol Agents of Postharvest Fungal Diseases in Lemons Perez, María Florencia Contreras, Luciana Garnica, Nydia Mercedes Fernández-Zenoff, María Verónica Farías, María Eugenia Sepulveda, Milena Ramallo, Jacqueline Dib, Julián Rafael PLoS One Research Article Economic losses caused by postharvest diseases represent one of the main problems of the citrus industry worldwide. The major diseases affecting citrus are the "green mold" and "blue mold", caused by Penicillium digitatum and P. italicum, respectively. To control them, synthetic fungicides are the most commonly used method. However, often the emergence of resistant strains occurs and their use is becoming more restricted because of toxic effects and environmental pollution they generate, combined with trade barriers to international markets. The aim of this work was to isolate indigenous killer yeasts with antagonistic activity against fungal postharvest diseases in lemons, and to determine their control efficiency in in vitro and in vivo assays. Among 437 yeast isolates, 8.5% show to have a killer phenotype. According to molecular identification, based on the 26S rDNA D1/D2 domain sequences analysis, strains were identified belonging to the genera Saccharomyces, Wickerhamomyces, Kazachstania, Pichia, Candida and Clavispora. Killers were challenged with pathogenic molds and strains that caused the maximum in vitro inhibition of P. digitatum were selected for in vivo assays. Two strains of Pichia and one strain of Wickerhamomyces depicted a significant protection (p <0.05) from decay by P. digitatum in assays using wounded lemons. Thus, the native killer yeasts studied in this work showed to be an effective alternative for the biocontrol of postharvest fungal infections of lemons and could be promising agents for the development of commercial products for the biological control industry. Public Library of Science 2016-10-28 /pmc/articles/PMC5085023/ /pubmed/27792761 http://dx.doi.org/10.1371/journal.pone.0165590 Text en © 2016 Perez et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Perez, María Florencia Contreras, Luciana Garnica, Nydia Mercedes Fernández-Zenoff, María Verónica Farías, María Eugenia Sepulveda, Milena Ramallo, Jacqueline Dib, Julián Rafael Native Killer Yeasts as Biocontrol Agents of Postharvest Fungal Diseases in Lemons |
title | Native Killer Yeasts as Biocontrol Agents of Postharvest Fungal Diseases in Lemons |
title_full | Native Killer Yeasts as Biocontrol Agents of Postharvest Fungal Diseases in Lemons |
title_fullStr | Native Killer Yeasts as Biocontrol Agents of Postharvest Fungal Diseases in Lemons |
title_full_unstemmed | Native Killer Yeasts as Biocontrol Agents of Postharvest Fungal Diseases in Lemons |
title_short | Native Killer Yeasts as Biocontrol Agents of Postharvest Fungal Diseases in Lemons |
title_sort | native killer yeasts as biocontrol agents of postharvest fungal diseases in lemons |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5085023/ https://www.ncbi.nlm.nih.gov/pubmed/27792761 http://dx.doi.org/10.1371/journal.pone.0165590 |
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