ns-μs Time-Resolved Step-Scan FTIR of ba(3) Oxidoreductase from Thermus thermophilus: Protonic Connectivity of w941-w946-w927

Time-resolved step-scan FTIR spectroscopy has been employed to probe the dynamics of the ba(3) oxidoreductase from Thermus thermophilus in the ns-μs time range and in the pH/pD 6–9 range. The data revealed a pH/pD sensitivity of the D372 residue and of the ring-A propionate of heme a(3). Based on th...

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Autores principales: Nicolaides, Antonis, Soulimane, Tewfik, Varotsis, Constantinos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5085690/
https://www.ncbi.nlm.nih.gov/pubmed/27690021
http://dx.doi.org/10.3390/ijms17101657
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author Nicolaides, Antonis
Soulimane, Tewfik
Varotsis, Constantinos
author_facet Nicolaides, Antonis
Soulimane, Tewfik
Varotsis, Constantinos
author_sort Nicolaides, Antonis
collection PubMed
description Time-resolved step-scan FTIR spectroscopy has been employed to probe the dynamics of the ba(3) oxidoreductase from Thermus thermophilus in the ns-μs time range and in the pH/pD 6–9 range. The data revealed a pH/pD sensitivity of the D372 residue and of the ring-A propionate of heme a(3). Based on the observed transient changes a model in which the protonic connectivity of w941-w946-927 to the D372 and the ring-A propionate of heme a(3) is described.
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spelling pubmed-50856902016-11-01 ns-μs Time-Resolved Step-Scan FTIR of ba(3) Oxidoreductase from Thermus thermophilus: Protonic Connectivity of w941-w946-w927 Nicolaides, Antonis Soulimane, Tewfik Varotsis, Constantinos Int J Mol Sci Article Time-resolved step-scan FTIR spectroscopy has been employed to probe the dynamics of the ba(3) oxidoreductase from Thermus thermophilus in the ns-μs time range and in the pH/pD 6–9 range. The data revealed a pH/pD sensitivity of the D372 residue and of the ring-A propionate of heme a(3). Based on the observed transient changes a model in which the protonic connectivity of w941-w946-927 to the D372 and the ring-A propionate of heme a(3) is described. MDPI 2016-09-29 /pmc/articles/PMC5085690/ /pubmed/27690021 http://dx.doi.org/10.3390/ijms17101657 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nicolaides, Antonis
Soulimane, Tewfik
Varotsis, Constantinos
ns-μs Time-Resolved Step-Scan FTIR of ba(3) Oxidoreductase from Thermus thermophilus: Protonic Connectivity of w941-w946-w927
title ns-μs Time-Resolved Step-Scan FTIR of ba(3) Oxidoreductase from Thermus thermophilus: Protonic Connectivity of w941-w946-w927
title_full ns-μs Time-Resolved Step-Scan FTIR of ba(3) Oxidoreductase from Thermus thermophilus: Protonic Connectivity of w941-w946-w927
title_fullStr ns-μs Time-Resolved Step-Scan FTIR of ba(3) Oxidoreductase from Thermus thermophilus: Protonic Connectivity of w941-w946-w927
title_full_unstemmed ns-μs Time-Resolved Step-Scan FTIR of ba(3) Oxidoreductase from Thermus thermophilus: Protonic Connectivity of w941-w946-w927
title_short ns-μs Time-Resolved Step-Scan FTIR of ba(3) Oxidoreductase from Thermus thermophilus: Protonic Connectivity of w941-w946-w927
title_sort ns-μs time-resolved step-scan ftir of ba(3) oxidoreductase from thermus thermophilus: protonic connectivity of w941-w946-w927
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5085690/
https://www.ncbi.nlm.nih.gov/pubmed/27690021
http://dx.doi.org/10.3390/ijms17101657
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