The Convenience of Single Homology Arm Donor DNA and CRISPR/Cas9-Nickase for Targeted Insertion of Long DNA Fragment

OBJECTIVE: CRISPR/Cas9 technology provides a powerful tool for targeted modification of genomes. In this system, a donor DNA harboring two flanking homology arms is mostly used for targeted insertion of long exogenous DNA. Here, we introduced an alternative design for the donor DNA by incorporation...

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Detalles Bibliográficos
Autores principales: Basiri, Mohsen, Behmanesh, Mehrdad, Tahamtani, Yaser, Khalooghi, Keynoosh, Moradmand, Azadeh, Baharvand, Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2017
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5086331/
https://www.ncbi.nlm.nih.gov/pubmed/28042537

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