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MicroRNA-146a Contributes to SCI Recovery via Regulating TRAF6 and IRAK1 Expression
MicroRNA-146a participates in spinal cord injury (SCI) recovery. Until recently, how miRNA-146a participates in SCI remained unclear. In this study, we tried to explore the roles of miRNA-146a in the recovery of SCI using a rat model. The expression of the probable target genes of miRNA-146a (includ...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5086513/ https://www.ncbi.nlm.nih.gov/pubmed/27830143 http://dx.doi.org/10.1155/2016/4013487 |
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author | Wei, Jinsong Wang, Jiafeng Zhou, Yulan Yan, Shouquan Li, Keshen Lin, Hongsheng |
author_facet | Wei, Jinsong Wang, Jiafeng Zhou, Yulan Yan, Shouquan Li, Keshen Lin, Hongsheng |
author_sort | Wei, Jinsong |
collection | PubMed |
description | MicroRNA-146a participates in spinal cord injury (SCI) recovery. Until recently, how miRNA-146a participates in SCI remained unclear. In this study, we tried to explore the roles of miRNA-146a in the recovery of SCI using a rat model. The expression of the probable target genes of miRNA-146a (including IRAK1 and TARF6) as well as proinflammation cytokines were measured until 7 days after surgery in the three groups (sham group, SCI group, and miRNA-146a antagomir injection group). Also, the animals' motivations were estimated using Basso Beattie Bresnahan (BBB) during the whole experiment. A luciferase assay was performed to demonstrate that miRNA-146a could directly target the mRNAs of IRAK1 and TRAF6. Our experiments indicate that miRNA-146a inhibits proinflammatory cytokine secretion by suppressing IRAK1 and TRAF6 expression in the SCI model. In contrast, miRNA-146a may be upregulated by inflammatory mediators via the IRAK1/TRAF6 pathway in the spinal cord. As a negative feedback element, miRNA-146a could make sure that the expression of IRAK1- and TRAF6-mediated genes was under tight control. Thus, miRNA-146a may serve as a novel therapeutic target for SCI interventions. |
format | Online Article Text |
id | pubmed-5086513 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-50865132016-11-09 MicroRNA-146a Contributes to SCI Recovery via Regulating TRAF6 and IRAK1 Expression Wei, Jinsong Wang, Jiafeng Zhou, Yulan Yan, Shouquan Li, Keshen Lin, Hongsheng Biomed Res Int Research Article MicroRNA-146a participates in spinal cord injury (SCI) recovery. Until recently, how miRNA-146a participates in SCI remained unclear. In this study, we tried to explore the roles of miRNA-146a in the recovery of SCI using a rat model. The expression of the probable target genes of miRNA-146a (including IRAK1 and TARF6) as well as proinflammation cytokines were measured until 7 days after surgery in the three groups (sham group, SCI group, and miRNA-146a antagomir injection group). Also, the animals' motivations were estimated using Basso Beattie Bresnahan (BBB) during the whole experiment. A luciferase assay was performed to demonstrate that miRNA-146a could directly target the mRNAs of IRAK1 and TRAF6. Our experiments indicate that miRNA-146a inhibits proinflammatory cytokine secretion by suppressing IRAK1 and TRAF6 expression in the SCI model. In contrast, miRNA-146a may be upregulated by inflammatory mediators via the IRAK1/TRAF6 pathway in the spinal cord. As a negative feedback element, miRNA-146a could make sure that the expression of IRAK1- and TRAF6-mediated genes was under tight control. Thus, miRNA-146a may serve as a novel therapeutic target for SCI interventions. Hindawi Publishing Corporation 2016 2016-10-17 /pmc/articles/PMC5086513/ /pubmed/27830143 http://dx.doi.org/10.1155/2016/4013487 Text en Copyright © 2016 Jinsong Wei et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wei, Jinsong Wang, Jiafeng Zhou, Yulan Yan, Shouquan Li, Keshen Lin, Hongsheng MicroRNA-146a Contributes to SCI Recovery via Regulating TRAF6 and IRAK1 Expression |
title | MicroRNA-146a Contributes to SCI Recovery via Regulating TRAF6 and IRAK1 Expression |
title_full | MicroRNA-146a Contributes to SCI Recovery via Regulating TRAF6 and IRAK1 Expression |
title_fullStr | MicroRNA-146a Contributes to SCI Recovery via Regulating TRAF6 and IRAK1 Expression |
title_full_unstemmed | MicroRNA-146a Contributes to SCI Recovery via Regulating TRAF6 and IRAK1 Expression |
title_short | MicroRNA-146a Contributes to SCI Recovery via Regulating TRAF6 and IRAK1 Expression |
title_sort | microrna-146a contributes to sci recovery via regulating traf6 and irak1 expression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5086513/ https://www.ncbi.nlm.nih.gov/pubmed/27830143 http://dx.doi.org/10.1155/2016/4013487 |
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