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Molecular Role of EGFR-MAPK Pathway in Patchouli Alcohol-Induced Apoptosis and Cell Cycle Arrest on A549 Cells In Vitro and In Vivo

Nowadays, chemotherapy is still the main effective treatment for cancer. Herb prescriptions containing Pogostemon cablin Benth (also known as “Guang-Huo-Xiang”) have been widely used in Chinese medicine today. In our research, we found that patchouli alcohol, a compound isolated from the oil of Pogo...

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Autores principales: Lu, XinGang, Yang, Liu, Lu, ChengHua, Xu, ZhenYu, Qiu, HongFu, Wu, JiaJia, Wang, JingWen, Tong, JiaFeng, Zhu, Yin, Shen, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5086517/
https://www.ncbi.nlm.nih.gov/pubmed/27830146
http://dx.doi.org/10.1155/2016/4567580
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author Lu, XinGang
Yang, Liu
Lu, ChengHua
Xu, ZhenYu
Qiu, HongFu
Wu, JiaJia
Wang, JingWen
Tong, JiaFeng
Zhu, Yin
Shen, Jie
author_facet Lu, XinGang
Yang, Liu
Lu, ChengHua
Xu, ZhenYu
Qiu, HongFu
Wu, JiaJia
Wang, JingWen
Tong, JiaFeng
Zhu, Yin
Shen, Jie
author_sort Lu, XinGang
collection PubMed
description Nowadays, chemotherapy is still the main effective treatment for cancer. Herb prescriptions containing Pogostemon cablin Benth (also known as “Guang-Huo-Xiang”) have been widely used in Chinese medicine today. In our research, we found that patchouli alcohol, a compound isolated from the oil of Pogostemon cablin Benth, exerted antitumor ability against human lung cancer A549 cells ability both in vitro and in vivo. MTT assay was used to assess cell viability. Hoechst 33342 staining and TUNEL cover glass staining provided the visual evidence of apoptosis. Caspase activity measurement showed that patchouli alcohol activated caspase 9 and caspase 3 of mitochondria-mediated apoptosis. Consistently, patchouli alcohol inhibited the xenograft tumor in vivo. Further investigation of the underlying molecular mechanism showed that MAPK and EGFR pathway might contribute to the antitumor effect of patchouli alcohol. Our study proved that patchouli alcohol might be able to serve as a novel antitumor compound in the clinical treatment of lung cancer.
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spelling pubmed-50865172016-11-09 Molecular Role of EGFR-MAPK Pathway in Patchouli Alcohol-Induced Apoptosis and Cell Cycle Arrest on A549 Cells In Vitro and In Vivo Lu, XinGang Yang, Liu Lu, ChengHua Xu, ZhenYu Qiu, HongFu Wu, JiaJia Wang, JingWen Tong, JiaFeng Zhu, Yin Shen, Jie Biomed Res Int Research Article Nowadays, chemotherapy is still the main effective treatment for cancer. Herb prescriptions containing Pogostemon cablin Benth (also known as “Guang-Huo-Xiang”) have been widely used in Chinese medicine today. In our research, we found that patchouli alcohol, a compound isolated from the oil of Pogostemon cablin Benth, exerted antitumor ability against human lung cancer A549 cells ability both in vitro and in vivo. MTT assay was used to assess cell viability. Hoechst 33342 staining and TUNEL cover glass staining provided the visual evidence of apoptosis. Caspase activity measurement showed that patchouli alcohol activated caspase 9 and caspase 3 of mitochondria-mediated apoptosis. Consistently, patchouli alcohol inhibited the xenograft tumor in vivo. Further investigation of the underlying molecular mechanism showed that MAPK and EGFR pathway might contribute to the antitumor effect of patchouli alcohol. Our study proved that patchouli alcohol might be able to serve as a novel antitumor compound in the clinical treatment of lung cancer. Hindawi Publishing Corporation 2016 2016-10-17 /pmc/articles/PMC5086517/ /pubmed/27830146 http://dx.doi.org/10.1155/2016/4567580 Text en Copyright © 2016 XinGang Lu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lu, XinGang
Yang, Liu
Lu, ChengHua
Xu, ZhenYu
Qiu, HongFu
Wu, JiaJia
Wang, JingWen
Tong, JiaFeng
Zhu, Yin
Shen, Jie
Molecular Role of EGFR-MAPK Pathway in Patchouli Alcohol-Induced Apoptosis and Cell Cycle Arrest on A549 Cells In Vitro and In Vivo
title Molecular Role of EGFR-MAPK Pathway in Patchouli Alcohol-Induced Apoptosis and Cell Cycle Arrest on A549 Cells In Vitro and In Vivo
title_full Molecular Role of EGFR-MAPK Pathway in Patchouli Alcohol-Induced Apoptosis and Cell Cycle Arrest on A549 Cells In Vitro and In Vivo
title_fullStr Molecular Role of EGFR-MAPK Pathway in Patchouli Alcohol-Induced Apoptosis and Cell Cycle Arrest on A549 Cells In Vitro and In Vivo
title_full_unstemmed Molecular Role of EGFR-MAPK Pathway in Patchouli Alcohol-Induced Apoptosis and Cell Cycle Arrest on A549 Cells In Vitro and In Vivo
title_short Molecular Role of EGFR-MAPK Pathway in Patchouli Alcohol-Induced Apoptosis and Cell Cycle Arrest on A549 Cells In Vitro and In Vivo
title_sort molecular role of egfr-mapk pathway in patchouli alcohol-induced apoptosis and cell cycle arrest on a549 cells in vitro and in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5086517/
https://www.ncbi.nlm.nih.gov/pubmed/27830146
http://dx.doi.org/10.1155/2016/4567580
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