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Protective Effects of Intratracheally-Administered Bee Venom Phospholipase A2 on Ovalbumin-Induced Allergic Asthma in Mice

Asthma is a common chronic disease characterized by bronchial inflammation, reversible airway obstruction, and airway hyperresponsiveness (AHR). Current therapeutic options for the management of asthma include inhaled corticosteroids and β2 agonists, which elicit harmful side effects. In the present...

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Autores principales: Jung, Kyung-Hwa, Baek, Hyunjung, Shin, Dasom, Lee, Gihyun, Park, Sangwon, Lee, Sujin, Choi, Dabin, Kim, Woojin, Bae, Hyunsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5086630/
https://www.ncbi.nlm.nih.gov/pubmed/27669297
http://dx.doi.org/10.3390/toxins8100269
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author Jung, Kyung-Hwa
Baek, Hyunjung
Shin, Dasom
Lee, Gihyun
Park, Sangwon
Lee, Sujin
Choi, Dabin
Kim, Woojin
Bae, Hyunsu
author_facet Jung, Kyung-Hwa
Baek, Hyunjung
Shin, Dasom
Lee, Gihyun
Park, Sangwon
Lee, Sujin
Choi, Dabin
Kim, Woojin
Bae, Hyunsu
author_sort Jung, Kyung-Hwa
collection PubMed
description Asthma is a common chronic disease characterized by bronchial inflammation, reversible airway obstruction, and airway hyperresponsiveness (AHR). Current therapeutic options for the management of asthma include inhaled corticosteroids and β2 agonists, which elicit harmful side effects. In the present study, we examined the capacity of phospholipase A2 (PLA2), one of the major components of bee venom (BV), to reduce airway inflammation and improve lung function in an experimental model of asthma. Allergic asthma was induced in female BALB/c mice by intraperitoneal administration of ovalbumin (OVA) on days 0 and 14, followed by intratracheal challenge with 1% OVA six times between days 22 and 30. The infiltration of immune cells, such as Th2 cytokines in the lungs, and the lung histology, were assessed in the OVA-challenged mice in the presence and absence of an intratracheal administration of bvPLA2. We showed that the intratracheal administration of bvPLA2 markedly suppressed the OVA-induced allergic airway inflammation by reducing AHR, overall area of inflammation, and goblet cell hyperplasia. Furthermore, the suppression was associated with a significant decrease in the production of Th2 cytokines, such as IL-4, IL-5, and IL-13, and a reduction in the number of total cells, including eosinophils, macrophages, and neutrophils in the airway.
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spelling pubmed-50866302016-11-02 Protective Effects of Intratracheally-Administered Bee Venom Phospholipase A2 on Ovalbumin-Induced Allergic Asthma in Mice Jung, Kyung-Hwa Baek, Hyunjung Shin, Dasom Lee, Gihyun Park, Sangwon Lee, Sujin Choi, Dabin Kim, Woojin Bae, Hyunsu Toxins (Basel) Article Asthma is a common chronic disease characterized by bronchial inflammation, reversible airway obstruction, and airway hyperresponsiveness (AHR). Current therapeutic options for the management of asthma include inhaled corticosteroids and β2 agonists, which elicit harmful side effects. In the present study, we examined the capacity of phospholipase A2 (PLA2), one of the major components of bee venom (BV), to reduce airway inflammation and improve lung function in an experimental model of asthma. Allergic asthma was induced in female BALB/c mice by intraperitoneal administration of ovalbumin (OVA) on days 0 and 14, followed by intratracheal challenge with 1% OVA six times between days 22 and 30. The infiltration of immune cells, such as Th2 cytokines in the lungs, and the lung histology, were assessed in the OVA-challenged mice in the presence and absence of an intratracheal administration of bvPLA2. We showed that the intratracheal administration of bvPLA2 markedly suppressed the OVA-induced allergic airway inflammation by reducing AHR, overall area of inflammation, and goblet cell hyperplasia. Furthermore, the suppression was associated with a significant decrease in the production of Th2 cytokines, such as IL-4, IL-5, and IL-13, and a reduction in the number of total cells, including eosinophils, macrophages, and neutrophils in the airway. MDPI 2016-09-22 /pmc/articles/PMC5086630/ /pubmed/27669297 http://dx.doi.org/10.3390/toxins8100269 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jung, Kyung-Hwa
Baek, Hyunjung
Shin, Dasom
Lee, Gihyun
Park, Sangwon
Lee, Sujin
Choi, Dabin
Kim, Woojin
Bae, Hyunsu
Protective Effects of Intratracheally-Administered Bee Venom Phospholipase A2 on Ovalbumin-Induced Allergic Asthma in Mice
title Protective Effects of Intratracheally-Administered Bee Venom Phospholipase A2 on Ovalbumin-Induced Allergic Asthma in Mice
title_full Protective Effects of Intratracheally-Administered Bee Venom Phospholipase A2 on Ovalbumin-Induced Allergic Asthma in Mice
title_fullStr Protective Effects of Intratracheally-Administered Bee Venom Phospholipase A2 on Ovalbumin-Induced Allergic Asthma in Mice
title_full_unstemmed Protective Effects of Intratracheally-Administered Bee Venom Phospholipase A2 on Ovalbumin-Induced Allergic Asthma in Mice
title_short Protective Effects of Intratracheally-Administered Bee Venom Phospholipase A2 on Ovalbumin-Induced Allergic Asthma in Mice
title_sort protective effects of intratracheally-administered bee venom phospholipase a2 on ovalbumin-induced allergic asthma in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5086630/
https://www.ncbi.nlm.nih.gov/pubmed/27669297
http://dx.doi.org/10.3390/toxins8100269
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