Quantitative Nuclear Magnetic Resonance Spectroscopy Based on PULCON Methodology: Application to Quantification of Invaluable Marine Toxin, Okadaic Acid

ERETIC2 (Electronic Reference To access In vivo Concentrations 2) based on PULCON (Pulse Length–based Concentration determination) methodology is a quantitative NMR (qNMR) using an external standard. The performance of the PULCON method was assessed using maleic acid (MA). Quantification of the diar...

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Autores principales: Watanabe, Ryuichi, Sugai, Chika, Yamazaki, Taichi, Matsushima, Ryoji, Uchida, Hajime, Matsumiya, Masahiro, Takatsu, Akiko, Suzuki, Toshiyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5086654/
https://www.ncbi.nlm.nih.gov/pubmed/27754382
http://dx.doi.org/10.3390/toxins8100294
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author Watanabe, Ryuichi
Sugai, Chika
Yamazaki, Taichi
Matsushima, Ryoji
Uchida, Hajime
Matsumiya, Masahiro
Takatsu, Akiko
Suzuki, Toshiyuki
author_facet Watanabe, Ryuichi
Sugai, Chika
Yamazaki, Taichi
Matsushima, Ryoji
Uchida, Hajime
Matsumiya, Masahiro
Takatsu, Akiko
Suzuki, Toshiyuki
author_sort Watanabe, Ryuichi
collection PubMed
description ERETIC2 (Electronic Reference To access In vivo Concentrations 2) based on PULCON (Pulse Length–based Concentration determination) methodology is a quantitative NMR (qNMR) using an external standard. The performance of the PULCON method was assessed using maleic acid (MA). Quantification of the diarrhetic shellfish toxin and okadaic acid by PULCON was successfully consistent with that obtained by a conventional internal standard method, demonstrating that the PULCON method is useful for the quantification of invaluable marine toxins without any contaminations by an internal standard.
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spelling pubmed-50866542016-11-02 Quantitative Nuclear Magnetic Resonance Spectroscopy Based on PULCON Methodology: Application to Quantification of Invaluable Marine Toxin, Okadaic Acid Watanabe, Ryuichi Sugai, Chika Yamazaki, Taichi Matsushima, Ryoji Uchida, Hajime Matsumiya, Masahiro Takatsu, Akiko Suzuki, Toshiyuki Toxins (Basel) Communication ERETIC2 (Electronic Reference To access In vivo Concentrations 2) based on PULCON (Pulse Length–based Concentration determination) methodology is a quantitative NMR (qNMR) using an external standard. The performance of the PULCON method was assessed using maleic acid (MA). Quantification of the diarrhetic shellfish toxin and okadaic acid by PULCON was successfully consistent with that obtained by a conventional internal standard method, demonstrating that the PULCON method is useful for the quantification of invaluable marine toxins without any contaminations by an internal standard. MDPI 2016-10-13 /pmc/articles/PMC5086654/ /pubmed/27754382 http://dx.doi.org/10.3390/toxins8100294 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Watanabe, Ryuichi
Sugai, Chika
Yamazaki, Taichi
Matsushima, Ryoji
Uchida, Hajime
Matsumiya, Masahiro
Takatsu, Akiko
Suzuki, Toshiyuki
Quantitative Nuclear Magnetic Resonance Spectroscopy Based on PULCON Methodology: Application to Quantification of Invaluable Marine Toxin, Okadaic Acid
title Quantitative Nuclear Magnetic Resonance Spectroscopy Based on PULCON Methodology: Application to Quantification of Invaluable Marine Toxin, Okadaic Acid
title_full Quantitative Nuclear Magnetic Resonance Spectroscopy Based on PULCON Methodology: Application to Quantification of Invaluable Marine Toxin, Okadaic Acid
title_fullStr Quantitative Nuclear Magnetic Resonance Spectroscopy Based on PULCON Methodology: Application to Quantification of Invaluable Marine Toxin, Okadaic Acid
title_full_unstemmed Quantitative Nuclear Magnetic Resonance Spectroscopy Based on PULCON Methodology: Application to Quantification of Invaluable Marine Toxin, Okadaic Acid
title_short Quantitative Nuclear Magnetic Resonance Spectroscopy Based on PULCON Methodology: Application to Quantification of Invaluable Marine Toxin, Okadaic Acid
title_sort quantitative nuclear magnetic resonance spectroscopy based on pulcon methodology: application to quantification of invaluable marine toxin, okadaic acid
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5086654/
https://www.ncbi.nlm.nih.gov/pubmed/27754382
http://dx.doi.org/10.3390/toxins8100294
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