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Expression of heparanase in basal cell carcinoma and squamous cell carcinoma

BACKGROUND: Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and squamous cell carcinoma comprise types of nonmelanoma skin cancer. OBJECTIVES: Evaluate the glycosaminoglycans profile and expression o...

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Autores principales: Pinhal, Maria Aparecida Silva, Almeida, Maria Carolina Leal, Costa, Alessandra Scorse, Theodoro, Thérèse Rachell, Serrano, Rodrigo Lorenzetti, Machado Filho, Carlos D'Apparecida Santos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Dermatologia 2016
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5087216/
https://www.ncbi.nlm.nih.gov/pubmed/27828631
http://dx.doi.org/10.1590/abd1806-4841.20164957
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author Pinhal, Maria Aparecida Silva
Almeida, Maria Carolina Leal
Costa, Alessandra Scorse
Theodoro, Thérèse Rachell
Serrano, Rodrigo Lorenzetti
Machado Filho, Carlos D'Apparecida Santos
author_facet Pinhal, Maria Aparecida Silva
Almeida, Maria Carolina Leal
Costa, Alessandra Scorse
Theodoro, Thérèse Rachell
Serrano, Rodrigo Lorenzetti
Machado Filho, Carlos D'Apparecida Santos
author_sort Pinhal, Maria Aparecida Silva
collection PubMed
description BACKGROUND: Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and squamous cell carcinoma comprise types of nonmelanoma skin cancer. OBJECTIVES: Evaluate the glycosaminoglycans profile and expression of heparanase in two human cell lines established in culture, immortalized skin keratinocyte (HaCaT) and squamous cell carcinoma (A431) and also investigate the expression of heparanase in basal cell carcinoma, squamous cell carcinoma and eyelid skin of individuals not affected by the disease (control). METHODS: Glycosaminoglycans were quantified by electrophoresis and indirect ELISA method. The heparanase expression was analyzed by quantitative RT-PCR (qRTPCR). RESULTS: The A431 strain showed significant increase in the sulfated glycosaminoglycans, increased heparanase expression and decreased hyaluronic acid, comparing to the HaCaT lineage. The mRNA expression of heparanase was significantly higher in Basal cell carcinoma and squamous cell carcinoma compared with control skin samples. It was also observed increased heparanase expression in squamous cell carcinoma compared to the Basal cell carcinoma. CONCLUSION: The glycosaminoglycans profile, as well as heparanase expression are different between HaCaT and A431 cell lines. The increased expression of heparanase in Basal cell carcinoma and squamous cell carcinoma suggests that this enzyme could be a marker for the diagnosis of such types of non-melanoma cancers, and may be useful as a target molecule for future alternative treatment.
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spelling pubmed-50872162016-11-01 Expression of heparanase in basal cell carcinoma and squamous cell carcinoma Pinhal, Maria Aparecida Silva Almeida, Maria Carolina Leal Costa, Alessandra Scorse Theodoro, Thérèse Rachell Serrano, Rodrigo Lorenzetti Machado Filho, Carlos D'Apparecida Santos An Bras Dermatol Investigation BACKGROUND: Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and squamous cell carcinoma comprise types of nonmelanoma skin cancer. OBJECTIVES: Evaluate the glycosaminoglycans profile and expression of heparanase in two human cell lines established in culture, immortalized skin keratinocyte (HaCaT) and squamous cell carcinoma (A431) and also investigate the expression of heparanase in basal cell carcinoma, squamous cell carcinoma and eyelid skin of individuals not affected by the disease (control). METHODS: Glycosaminoglycans were quantified by electrophoresis and indirect ELISA method. The heparanase expression was analyzed by quantitative RT-PCR (qRTPCR). RESULTS: The A431 strain showed significant increase in the sulfated glycosaminoglycans, increased heparanase expression and decreased hyaluronic acid, comparing to the HaCaT lineage. The mRNA expression of heparanase was significantly higher in Basal cell carcinoma and squamous cell carcinoma compared with control skin samples. It was also observed increased heparanase expression in squamous cell carcinoma compared to the Basal cell carcinoma. CONCLUSION: The glycosaminoglycans profile, as well as heparanase expression are different between HaCaT and A431 cell lines. The increased expression of heparanase in Basal cell carcinoma and squamous cell carcinoma suggests that this enzyme could be a marker for the diagnosis of such types of non-melanoma cancers, and may be useful as a target molecule for future alternative treatment. Sociedade Brasileira de Dermatologia 2016 /pmc/articles/PMC5087216/ /pubmed/27828631 http://dx.doi.org/10.1590/abd1806-4841.20164957 Text en ©2016 by Anais Brasileiros de Dermatologia http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium provided the original work is properly cited.
spellingShingle Investigation
Pinhal, Maria Aparecida Silva
Almeida, Maria Carolina Leal
Costa, Alessandra Scorse
Theodoro, Thérèse Rachell
Serrano, Rodrigo Lorenzetti
Machado Filho, Carlos D'Apparecida Santos
Expression of heparanase in basal cell carcinoma and squamous cell carcinoma
title Expression of heparanase in basal cell carcinoma and squamous cell carcinoma
title_full Expression of heparanase in basal cell carcinoma and squamous cell carcinoma
title_fullStr Expression of heparanase in basal cell carcinoma and squamous cell carcinoma
title_full_unstemmed Expression of heparanase in basal cell carcinoma and squamous cell carcinoma
title_short Expression of heparanase in basal cell carcinoma and squamous cell carcinoma
title_sort expression of heparanase in basal cell carcinoma and squamous cell carcinoma
topic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5087216/
https://www.ncbi.nlm.nih.gov/pubmed/27828631
http://dx.doi.org/10.1590/abd1806-4841.20164957
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