A MoS(2) Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation

MoS(2) nanomaterial has unique properties, including innate affinity with ss-DNA and quenching ability for fluorescence dyes. Here, we present the development of a simple fluorescence biosensor based on water-soluble MoS(2) nanosheets and restriction endonuclease BstUI for methylation analysis of p1...

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Detalles Bibliográficos
Autores principales: Xiao, Le, Xu, Li, Gao, Chuan, Zhang, Yulin, Yao, Qunfeng, Zhang, Guo-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5087350/
https://www.ncbi.nlm.nih.gov/pubmed/27669248
http://dx.doi.org/10.3390/s16101561
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author Xiao, Le
Xu, Li
Gao, Chuan
Zhang, Yulin
Yao, Qunfeng
Zhang, Guo-Jun
author_facet Xiao, Le
Xu, Li
Gao, Chuan
Zhang, Yulin
Yao, Qunfeng
Zhang, Guo-Jun
author_sort Xiao, Le
collection PubMed
description MoS(2) nanomaterial has unique properties, including innate affinity with ss-DNA and quenching ability for fluorescence dyes. Here, we present the development of a simple fluorescence biosensor based on water-soluble MoS(2) nanosheets and restriction endonuclease BstUI for methylation analysis of p16 promoter. The biosensing platform exhibited excellent sensitivity in detecting DNA with a linear range of 100 pM~20 nM and a detection limit of 140 pM. More importantly, our method could distinguish as low as 1% difference in methylation level. Compared with previous methylation analysis, our design is both time saving and simple to operate, avoiding the limitations of PCR-based assays without compromising performance.
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spelling pubmed-50873502016-11-07 A MoS(2) Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation Xiao, Le Xu, Li Gao, Chuan Zhang, Yulin Yao, Qunfeng Zhang, Guo-Jun Sensors (Basel) Article MoS(2) nanomaterial has unique properties, including innate affinity with ss-DNA and quenching ability for fluorescence dyes. Here, we present the development of a simple fluorescence biosensor based on water-soluble MoS(2) nanosheets and restriction endonuclease BstUI for methylation analysis of p16 promoter. The biosensing platform exhibited excellent sensitivity in detecting DNA with a linear range of 100 pM~20 nM and a detection limit of 140 pM. More importantly, our method could distinguish as low as 1% difference in methylation level. Compared with previous methylation analysis, our design is both time saving and simple to operate, avoiding the limitations of PCR-based assays without compromising performance. MDPI 2016-09-22 /pmc/articles/PMC5087350/ /pubmed/27669248 http://dx.doi.org/10.3390/s16101561 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xiao, Le
Xu, Li
Gao, Chuan
Zhang, Yulin
Yao, Qunfeng
Zhang, Guo-Jun
A MoS(2) Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation
title A MoS(2) Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation
title_full A MoS(2) Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation
title_fullStr A MoS(2) Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation
title_full_unstemmed A MoS(2) Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation
title_short A MoS(2) Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation
title_sort mos(2) nanosheet-based fluorescence biosensor for simple and quantitative analysis of dna methylation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5087350/
https://www.ncbi.nlm.nih.gov/pubmed/27669248
http://dx.doi.org/10.3390/s16101561
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