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Matriptase and prostasin are expressed in human skin in an inverse trend over the course of differentiation and are targeted to different regions of the plasma membrane

Matriptase and prostasin, acting as a tightly coupled proteolytic cascade, were reported to be required for epidermal barrier formation in mouse skin. Here we show that, in human skin, matriptase and prostasin are expressed with an inverse pattern over the course of differentiation. Matriptase was d...

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Autores principales: Lai, Chih-Hsin, Chang, Shun-Cheng, Chen, Yen-Ju, Wang, Yi-Jie J., Lai, Ying-Jun J., Chang, Hsiang-Hua D., Berens, Eric B., Johnson, Michael D., Wang, Jehng-Kang, Lin, Chen-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5087689/
https://www.ncbi.nlm.nih.gov/pubmed/27543057
http://dx.doi.org/10.1242/bio.019745
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author Lai, Chih-Hsin
Chang, Shun-Cheng
Chen, Yen-Ju
Wang, Yi-Jie J.
Lai, Ying-Jun J.
Chang, Hsiang-Hua D.
Berens, Eric B.
Johnson, Michael D.
Wang, Jehng-Kang
Lin, Chen-Yong
author_facet Lai, Chih-Hsin
Chang, Shun-Cheng
Chen, Yen-Ju
Wang, Yi-Jie J.
Lai, Ying-Jun J.
Chang, Hsiang-Hua D.
Berens, Eric B.
Johnson, Michael D.
Wang, Jehng-Kang
Lin, Chen-Yong
author_sort Lai, Chih-Hsin
collection PubMed
description Matriptase and prostasin, acting as a tightly coupled proteolytic cascade, were reported to be required for epidermal barrier formation in mouse skin. Here we show that, in human skin, matriptase and prostasin are expressed with an inverse pattern over the course of differentiation. Matriptase was detected primarily in epidermal basal keratinocytes and the basaloid cells in the outer root sheath of hair follicles and the sebaceous gland, where prostasin was not detected. In contrast, prostasin was detected primarily in differentiated cells in the epidermal granular layer, the inner root sheath of hair follicles, and the sebaceous gland, where matriptase expression is negligible. While co-expressed in the middle stage of differentiation, prostasin was detected as polarized patches, and matriptase at intercellular junctions. Targeting to different subcellular localizations is also observed in HaCaT human keratinocytes, in which matriptase was detected primarily at intercellular junctions, and prostasin primarily on membrane protrusion. Furthermore, upon induction of zymogen activation, free active prostasin remains cell-associated and free active matriptase is rapidly shed into the extracellular milieu. Our data suggest that matriptase and prostasin likely function as independent entities in human skin rather than as a tightly coupled proteolytic cascade as observed in mouse skin.
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spelling pubmed-50876892016-10-31 Matriptase and prostasin are expressed in human skin in an inverse trend over the course of differentiation and are targeted to different regions of the plasma membrane Lai, Chih-Hsin Chang, Shun-Cheng Chen, Yen-Ju Wang, Yi-Jie J. Lai, Ying-Jun J. Chang, Hsiang-Hua D. Berens, Eric B. Johnson, Michael D. Wang, Jehng-Kang Lin, Chen-Yong Biol Open Research Article Matriptase and prostasin, acting as a tightly coupled proteolytic cascade, were reported to be required for epidermal barrier formation in mouse skin. Here we show that, in human skin, matriptase and prostasin are expressed with an inverse pattern over the course of differentiation. Matriptase was detected primarily in epidermal basal keratinocytes and the basaloid cells in the outer root sheath of hair follicles and the sebaceous gland, where prostasin was not detected. In contrast, prostasin was detected primarily in differentiated cells in the epidermal granular layer, the inner root sheath of hair follicles, and the sebaceous gland, where matriptase expression is negligible. While co-expressed in the middle stage of differentiation, prostasin was detected as polarized patches, and matriptase at intercellular junctions. Targeting to different subcellular localizations is also observed in HaCaT human keratinocytes, in which matriptase was detected primarily at intercellular junctions, and prostasin primarily on membrane protrusion. Furthermore, upon induction of zymogen activation, free active prostasin remains cell-associated and free active matriptase is rapidly shed into the extracellular milieu. Our data suggest that matriptase and prostasin likely function as independent entities in human skin rather than as a tightly coupled proteolytic cascade as observed in mouse skin. The Company of Biologists Ltd 2016-08-19 /pmc/articles/PMC5087689/ /pubmed/27543057 http://dx.doi.org/10.1242/bio.019745 Text en © 2016. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Lai, Chih-Hsin
Chang, Shun-Cheng
Chen, Yen-Ju
Wang, Yi-Jie J.
Lai, Ying-Jun J.
Chang, Hsiang-Hua D.
Berens, Eric B.
Johnson, Michael D.
Wang, Jehng-Kang
Lin, Chen-Yong
Matriptase and prostasin are expressed in human skin in an inverse trend over the course of differentiation and are targeted to different regions of the plasma membrane
title Matriptase and prostasin are expressed in human skin in an inverse trend over the course of differentiation and are targeted to different regions of the plasma membrane
title_full Matriptase and prostasin are expressed in human skin in an inverse trend over the course of differentiation and are targeted to different regions of the plasma membrane
title_fullStr Matriptase and prostasin are expressed in human skin in an inverse trend over the course of differentiation and are targeted to different regions of the plasma membrane
title_full_unstemmed Matriptase and prostasin are expressed in human skin in an inverse trend over the course of differentiation and are targeted to different regions of the plasma membrane
title_short Matriptase and prostasin are expressed in human skin in an inverse trend over the course of differentiation and are targeted to different regions of the plasma membrane
title_sort matriptase and prostasin are expressed in human skin in an inverse trend over the course of differentiation and are targeted to different regions of the plasma membrane
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5087689/
https://www.ncbi.nlm.nih.gov/pubmed/27543057
http://dx.doi.org/10.1242/bio.019745
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