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Vibrio vulnificus Type 6 Secretion System 1 Contains Anti-Bacterial Properties
Vibrio vulnificus is a bacterium responsible for severe gastroenteritis, sepsis and wound infections. Gastroenteritis and sepsis are commonly associated with the consumption of raw oysters, whereas wound infection is often associated with the handling of contaminated fish. Although classical virulen...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5087951/ https://www.ncbi.nlm.nih.gov/pubmed/27798649 http://dx.doi.org/10.1371/journal.pone.0165500 |
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author | Church, Selina R. Lux, Thomas Baker-Austin, Craig Buddington, Sam P. Michell, Stephen Ll. |
author_facet | Church, Selina R. Lux, Thomas Baker-Austin, Craig Buddington, Sam P. Michell, Stephen Ll. |
author_sort | Church, Selina R. |
collection | PubMed |
description | Vibrio vulnificus is a bacterium responsible for severe gastroenteritis, sepsis and wound infections. Gastroenteritis and sepsis are commonly associated with the consumption of raw oysters, whereas wound infection is often associated with the handling of contaminated fish. Although classical virulence factors of this emerging pathogen are well characterised, there remains a paucity of knowledge regarding the general biology of this species. To investigate the presence of previously unreported virulence factors, we applied whole genome sequencing to a panel of ten V. vulnificus strains with varying virulence potentials. This identified two novel type 6 secretion systems (T6SSs), systems that are known to have a role in bacterial virulence and population dynamics. By utilising a range of molecular techniques and assays we have demonstrated the functionality of one of these T6SSs. Furthermore, we have shown that this system is subject to thermoregulation and is negatively regulated by increasing salinity concentrations. This secretion system was also shown to be involved in the killing of V. vulnificus strains that did not possess this system and a model is proposed as to how this interaction may contribute to population dynamics within V. vulnificus strains. In addition to this intra-species killing, this system also contributes to the killing of inter bacterial species and may have a role in the general composition of Vibrio species in the environment. |
format | Online Article Text |
id | pubmed-5087951 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-50879512016-11-15 Vibrio vulnificus Type 6 Secretion System 1 Contains Anti-Bacterial Properties Church, Selina R. Lux, Thomas Baker-Austin, Craig Buddington, Sam P. Michell, Stephen Ll. PLoS One Research Article Vibrio vulnificus is a bacterium responsible for severe gastroenteritis, sepsis and wound infections. Gastroenteritis and sepsis are commonly associated with the consumption of raw oysters, whereas wound infection is often associated with the handling of contaminated fish. Although classical virulence factors of this emerging pathogen are well characterised, there remains a paucity of knowledge regarding the general biology of this species. To investigate the presence of previously unreported virulence factors, we applied whole genome sequencing to a panel of ten V. vulnificus strains with varying virulence potentials. This identified two novel type 6 secretion systems (T6SSs), systems that are known to have a role in bacterial virulence and population dynamics. By utilising a range of molecular techniques and assays we have demonstrated the functionality of one of these T6SSs. Furthermore, we have shown that this system is subject to thermoregulation and is negatively regulated by increasing salinity concentrations. This secretion system was also shown to be involved in the killing of V. vulnificus strains that did not possess this system and a model is proposed as to how this interaction may contribute to population dynamics within V. vulnificus strains. In addition to this intra-species killing, this system also contributes to the killing of inter bacterial species and may have a role in the general composition of Vibrio species in the environment. Public Library of Science 2016-10-31 /pmc/articles/PMC5087951/ /pubmed/27798649 http://dx.doi.org/10.1371/journal.pone.0165500 Text en © 2016 Church et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Church, Selina R. Lux, Thomas Baker-Austin, Craig Buddington, Sam P. Michell, Stephen Ll. Vibrio vulnificus Type 6 Secretion System 1 Contains Anti-Bacterial Properties |
title | Vibrio vulnificus Type 6 Secretion System 1 Contains Anti-Bacterial Properties |
title_full | Vibrio vulnificus Type 6 Secretion System 1 Contains Anti-Bacterial Properties |
title_fullStr | Vibrio vulnificus Type 6 Secretion System 1 Contains Anti-Bacterial Properties |
title_full_unstemmed | Vibrio vulnificus Type 6 Secretion System 1 Contains Anti-Bacterial Properties |
title_short | Vibrio vulnificus Type 6 Secretion System 1 Contains Anti-Bacterial Properties |
title_sort | vibrio vulnificus type 6 secretion system 1 contains anti-bacterial properties |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5087951/ https://www.ncbi.nlm.nih.gov/pubmed/27798649 http://dx.doi.org/10.1371/journal.pone.0165500 |
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