Mate-pair editing: a perspective to double mate-pair sequencing coverage

In this report, I am proposing a hypothetical approach that can enable sequencing of four short reads from the same insert using Illumina next-generation sequencing. The methodology is similar to that used in mate-pair sequencing except that it involves two circularization steps and the sequencing s...

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Detalles Bibliográficos
Autor principal: Jighly, Abdulqader
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088180/
https://www.ncbi.nlm.nih.gov/pubmed/28330306
http://dx.doi.org/10.1007/s13205-016-0554-z
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author Jighly, Abdulqader
author_facet Jighly, Abdulqader
author_sort Jighly, Abdulqader
collection PubMed
description In this report, I am proposing a hypothetical approach that can enable sequencing of four short reads from the same insert using Illumina next-generation sequencing. The methodology is similar to that used in mate-pair sequencing except that it involves two circularization steps and the sequencing slide should have four different oligonucleotides.
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spelling pubmed-50881802016-11-01 Mate-pair editing: a perspective to double mate-pair sequencing coverage Jighly, Abdulqader 3 Biotech Letter to the Editor In this report, I am proposing a hypothetical approach that can enable sequencing of four short reads from the same insert using Illumina next-generation sequencing. The methodology is similar to that used in mate-pair sequencing except that it involves two circularization steps and the sequencing slide should have four different oligonucleotides. Springer Berlin Heidelberg 2016-10-31 2016-12 /pmc/articles/PMC5088180/ /pubmed/28330306 http://dx.doi.org/10.1007/s13205-016-0554-z Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Letter to the Editor
Jighly, Abdulqader
Mate-pair editing: a perspective to double mate-pair sequencing coverage
title Mate-pair editing: a perspective to double mate-pair sequencing coverage
title_full Mate-pair editing: a perspective to double mate-pair sequencing coverage
title_fullStr Mate-pair editing: a perspective to double mate-pair sequencing coverage
title_full_unstemmed Mate-pair editing: a perspective to double mate-pair sequencing coverage
title_short Mate-pair editing: a perspective to double mate-pair sequencing coverage
title_sort mate-pair editing: a perspective to double mate-pair sequencing coverage
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088180/
https://www.ncbi.nlm.nih.gov/pubmed/28330306
http://dx.doi.org/10.1007/s13205-016-0554-z
work_keys_str_mv AT jighlyabdulqader matepaireditingaperspectivetodoublematepairsequencingcoverage

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