Purification of Bioactive Lipopeptides Produced by Bacillus subtilis Strain BIA

Bacillus subtilis strain BIA was used for the production of bioactive lipopeptides. Different extraction and purification methods were assayed as liquid–liquid extraction, and acid and ammonium sulfate precipitation followed by TLC, SPE, and gel filtration. Active fractions were further purified usi...

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Autores principales: Alajlani, Muaaz, Shiekh, Abid, Hasnain, Shahida, Brantner, Adelheid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Original
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088217/
https://www.ncbi.nlm.nih.gov/pubmed/27867207
http://dx.doi.org/10.1007/s10337-016-3164-3
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author Alajlani, Muaaz
Shiekh, Abid
Hasnain, Shahida
Brantner, Adelheid
author_facet Alajlani, Muaaz
Shiekh, Abid
Hasnain, Shahida
Brantner, Adelheid
author_sort Alajlani, Muaaz
collection PubMed
description Bacillus subtilis strain BIA was used for the production of bioactive lipopeptides. Different extraction and purification methods were assayed as liquid–liquid extraction, and acid and ammonium sulfate precipitation followed by TLC, SPE, and gel filtration. Active fractions were further purified using RP-HPLC. The molecular mass of the purified product from HPLC was determined through Tris-Tricine SDS-PAGE and MALDI–TOF-MS. The results revealed that Bacillus subtilis strain BIA produced surfactin and iturin like compounds. Coproduction of surfactin and iturin like compounds by this strain is a remarkable trait for a potential biocontrol agent. This paper also includeds techniques that have been developed for the optimal and convenient extraction of bioactive lipopeptides from microbial origin.
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spelling pubmed-50882172016-11-16 Purification of Bioactive Lipopeptides Produced by Bacillus subtilis Strain BIA Alajlani, Muaaz Shiekh, Abid Hasnain, Shahida Brantner, Adelheid Chromatographia Original Bacillus subtilis strain BIA was used for the production of bioactive lipopeptides. Different extraction and purification methods were assayed as liquid–liquid extraction, and acid and ammonium sulfate precipitation followed by TLC, SPE, and gel filtration. Active fractions were further purified using RP-HPLC. The molecular mass of the purified product from HPLC was determined through Tris-Tricine SDS-PAGE and MALDI–TOF-MS. The results revealed that Bacillus subtilis strain BIA produced surfactin and iturin like compounds. Coproduction of surfactin and iturin like compounds by this strain is a remarkable trait for a potential biocontrol agent. This paper also includeds techniques that have been developed for the optimal and convenient extraction of bioactive lipopeptides from microbial origin. Springer Berlin Heidelberg 2016-09-17 2016 /pmc/articles/PMC5088217/ /pubmed/27867207 http://dx.doi.org/10.1007/s10337-016-3164-3 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original
Alajlani, Muaaz
Shiekh, Abid
Hasnain, Shahida
Brantner, Adelheid
Purification of Bioactive Lipopeptides Produced by Bacillus subtilis Strain BIA
title Purification of Bioactive Lipopeptides Produced by Bacillus subtilis Strain BIA
title_full Purification of Bioactive Lipopeptides Produced by Bacillus subtilis Strain BIA
title_fullStr Purification of Bioactive Lipopeptides Produced by Bacillus subtilis Strain BIA
title_full_unstemmed Purification of Bioactive Lipopeptides Produced by Bacillus subtilis Strain BIA
title_short Purification of Bioactive Lipopeptides Produced by Bacillus subtilis Strain BIA
title_sort purification of bioactive lipopeptides produced by bacillus subtilis strain bia
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088217/
https://www.ncbi.nlm.nih.gov/pubmed/27867207
http://dx.doi.org/10.1007/s10337-016-3164-3
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