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Poly-N-isopropylacrylamide (pNIPAM): a reversible bioadhesive for sclerotomy closure
PURPOSE: To determine the safety and efficacy of poly-N-isopropylacrylamide, a thermoresponsive adhesive, for sutureless sclerotomy closure in rabbits. METHODS: Eight rabbits were randomized into three groups: short-term acute, mid-term chronic, and long-term chronic studies. A corresponding control...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088454/ https://www.ncbi.nlm.nih.gov/pubmed/27847641 http://dx.doi.org/10.1186/s40942-016-0048-5 |
Sumario: | PURPOSE: To determine the safety and efficacy of poly-N-isopropylacrylamide, a thermoresponsive adhesive, for sutureless sclerotomy closure in rabbits. METHODS: Eight rabbits were randomized into three groups: short-term acute, mid-term chronic, and long-term chronic studies. A corresponding control group in which the scleral wounds were sutured by 6–0 vicryl sutures was assigned for each study group. A 20-gauge sclerotomy was performed following a core vitrectomy and 0.1 mL of 50 % liquid poly-N-isopropylacrylamide was applied to the scleral wounds. Before the polymer application, the scleral surface was raised above 32 °C using a halogen bulb lamp. Follow-up exams included color external and fundus photography, fundus fluorescein angiography, optical coherence tomography, and electroretinography. After the last follow-up assessment, the rabbits were sacrificed and histopathological studies on the scleral incision sites were performed. RESULTS: Scleral wound healing was observed in the long-term chronic study rabbits. Histological studies were able to identify poly-N-isopropylacrylamide polymer at the sclerotomy site in the mid-term chronic study rabbits. Besides iatrogenic cataracts due to mechanical instrument touch in 2 rabbits, no other ocular abnormalities were identified in any of the eyes in the perioperative setting or during the follow-up period. Cornea, retina, and vitreous remained unaffected, and no abnormal inflammatory reaction or endophthalmitis was noticed in the 3 study groups. Filtering blebs indicative of leakage through the sclerotomies were not observed during the follow-up period. CONCLUSION: Poly-N-isopropylacrylamide may provide effective in vitro scleral adhesion above 32 °C. Clinical studies are required to evaluate its utility in patients undergoing pars plana vitrectomy. |
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