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Optical Detection of Early Damage in Retinal Ganglion Cells in a Mouse Model of Partial Optic Nerve Crush Injury

PURPOSE: Elastic light backscattering spectroscopy (ELBS) has exquisite sensitivity to the ultrastructural properties of tissue and thus has been applied to detect various diseases associated with ultrastructural alterations in their early stages. This study aims to test whether ELBS can detect earl...

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Autores principales: Yi, Ji, Puyang, Zhen, Feng, Liang, Duan, Lian, Liang, Peiji, Backman, Vadim, Liu, Xiaorong, Zhang, Hao F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5089219/
https://www.ncbi.nlm.nih.gov/pubmed/27784071
http://dx.doi.org/10.1167/iovs.16-19955
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author Yi, Ji
Puyang, Zhen
Feng, Liang
Duan, Lian
Liang, Peiji
Backman, Vadim
Liu, Xiaorong
Zhang, Hao F.
author_facet Yi, Ji
Puyang, Zhen
Feng, Liang
Duan, Lian
Liang, Peiji
Backman, Vadim
Liu, Xiaorong
Zhang, Hao F.
author_sort Yi, Ji
collection PubMed
description PURPOSE: Elastic light backscattering spectroscopy (ELBS) has exquisite sensitivity to the ultrastructural properties of tissue and thus has been applied to detect various diseases associated with ultrastructural alterations in their early stages. This study aims to test whether ELBS can detect early damage in retinal ganglion cells (RGCs). METHODS: We used a mouse model of partial optic nerve crush (pONC) to induce rapid RGC death. We confirmed RGC loss by axon counting and characterized the changes in retinal morphology by optical coherence tomography (OCT) and in retinal function by full-field electroretinogram (ERG), respectively. To quantify the ultrastructural properties, elastic backscattering spectroscopic analysis was implemented in the wavelength-dependent images recorded by reflectance confocal microscopy. RESULTS: At 3 days post-pONC injury, no significant change was found in the thickness of the RGC layer or in the mean amplitude of the oscillatory potentials measured by OCT and ERG, respectively; however, we did observe a significantly decreased number of axons compared with the controls. At 3 days post-pONC, we used ELBS to calculate the ultrastructural marker (D), the shape factor quantifying the shape of the local mass density correlation functions. It was significantly reduced in the crushed eyes compared with the controls, indicating the ultrastructural fragmentation in the crushed eyes. CONCLUSIONS: Elastic light backscattering spectroscopy detected ultrastructural neuronal damage in RGCs following the pONC injury when OCT and ERG tests appeared normal. Our study suggests a potential clinical method for detecting early neuronal damage prior to anatomical alterations in the nerve fiber and ganglion cell layers.
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spelling pubmed-50892192016-11-06 Optical Detection of Early Damage in Retinal Ganglion Cells in a Mouse Model of Partial Optic Nerve Crush Injury Yi, Ji Puyang, Zhen Feng, Liang Duan, Lian Liang, Peiji Backman, Vadim Liu, Xiaorong Zhang, Hao F. Invest Ophthalmol Vis Sci Multidisciplinary Ophthalmic Imaging PURPOSE: Elastic light backscattering spectroscopy (ELBS) has exquisite sensitivity to the ultrastructural properties of tissue and thus has been applied to detect various diseases associated with ultrastructural alterations in their early stages. This study aims to test whether ELBS can detect early damage in retinal ganglion cells (RGCs). METHODS: We used a mouse model of partial optic nerve crush (pONC) to induce rapid RGC death. We confirmed RGC loss by axon counting and characterized the changes in retinal morphology by optical coherence tomography (OCT) and in retinal function by full-field electroretinogram (ERG), respectively. To quantify the ultrastructural properties, elastic backscattering spectroscopic analysis was implemented in the wavelength-dependent images recorded by reflectance confocal microscopy. RESULTS: At 3 days post-pONC injury, no significant change was found in the thickness of the RGC layer or in the mean amplitude of the oscillatory potentials measured by OCT and ERG, respectively; however, we did observe a significantly decreased number of axons compared with the controls. At 3 days post-pONC, we used ELBS to calculate the ultrastructural marker (D), the shape factor quantifying the shape of the local mass density correlation functions. It was significantly reduced in the crushed eyes compared with the controls, indicating the ultrastructural fragmentation in the crushed eyes. CONCLUSIONS: Elastic light backscattering spectroscopy detected ultrastructural neuronal damage in RGCs following the pONC injury when OCT and ERG tests appeared normal. Our study suggests a potential clinical method for detecting early neuronal damage prior to anatomical alterations in the nerve fiber and ganglion cell layers. The Association for Research in Vision and Ophthalmology 2016-10 /pmc/articles/PMC5089219/ /pubmed/27784071 http://dx.doi.org/10.1167/iovs.16-19955 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Multidisciplinary Ophthalmic Imaging
Yi, Ji
Puyang, Zhen
Feng, Liang
Duan, Lian
Liang, Peiji
Backman, Vadim
Liu, Xiaorong
Zhang, Hao F.
Optical Detection of Early Damage in Retinal Ganglion Cells in a Mouse Model of Partial Optic Nerve Crush Injury
title Optical Detection of Early Damage in Retinal Ganglion Cells in a Mouse Model of Partial Optic Nerve Crush Injury
title_full Optical Detection of Early Damage in Retinal Ganglion Cells in a Mouse Model of Partial Optic Nerve Crush Injury
title_fullStr Optical Detection of Early Damage in Retinal Ganglion Cells in a Mouse Model of Partial Optic Nerve Crush Injury
title_full_unstemmed Optical Detection of Early Damage in Retinal Ganglion Cells in a Mouse Model of Partial Optic Nerve Crush Injury
title_short Optical Detection of Early Damage in Retinal Ganglion Cells in a Mouse Model of Partial Optic Nerve Crush Injury
title_sort optical detection of early damage in retinal ganglion cells in a mouse model of partial optic nerve crush injury
topic Multidisciplinary Ophthalmic Imaging
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5089219/
https://www.ncbi.nlm.nih.gov/pubmed/27784071
http://dx.doi.org/10.1167/iovs.16-19955
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