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Genome-Wide Association of Stem Water Soluble Carbohydrates in Bread Wheat
Water soluble carbohydrates (WSC) in stems play an important role in buffering grain yield in wheat against biotic and abiotic stresses; however, knowledge of genes controlling WSC is very limited. We conducted a genome-wide association study (GWAS) using a high-density 90K SNP array to better under...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5089554/ https://www.ncbi.nlm.nih.gov/pubmed/27802269 http://dx.doi.org/10.1371/journal.pone.0164293 |
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author | Dong, Yan Liu, Jindong Zhang, Yan Geng, Hongwei Rasheed, Awais Xiao, Yonggui Cao, Shuanghe Fu, Luping Yan, Jun Wen, Weie Zhang, Yong Jing, Ruilian Xia, Xianchun He, Zhonghu |
author_facet | Dong, Yan Liu, Jindong Zhang, Yan Geng, Hongwei Rasheed, Awais Xiao, Yonggui Cao, Shuanghe Fu, Luping Yan, Jun Wen, Weie Zhang, Yong Jing, Ruilian Xia, Xianchun He, Zhonghu |
author_sort | Dong, Yan |
collection | PubMed |
description | Water soluble carbohydrates (WSC) in stems play an important role in buffering grain yield in wheat against biotic and abiotic stresses; however, knowledge of genes controlling WSC is very limited. We conducted a genome-wide association study (GWAS) using a high-density 90K SNP array to better understand the genetic basis underlying WSC, and to explore marker-based breeding approaches. WSC was evaluated in an association panel comprising 166 Chinese bread wheat cultivars planted in four environments. Fifty two marker-trait associations (MTAs) distributed across 23 loci were identified for phenotypic best linear unbiased estimates (BLUEs), and 11 MTAs were identified in two or more environments. Liner regression showed a clear dependence of WSC BLUE scores on numbers of favorable (increasing WSC content) and unfavorable alleles (decreasing WSC), indicating that genotypes with higher numbers of favorable or lower numbers of unfavorable alleles had higher WSC content. In silico analysis of flanking sequences of trait-associated SNPs revealed eight candidate genes related to WSC content grouped into two categories based on the type of encoding proteins, namely, defense response proteins and proteins triggered by environmental stresses. The identified SNPs and candidate genes related to WSC provide opportunities for breeding higher WSC wheat cultivars. |
format | Online Article Text |
id | pubmed-5089554 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-50895542016-11-15 Genome-Wide Association of Stem Water Soluble Carbohydrates in Bread Wheat Dong, Yan Liu, Jindong Zhang, Yan Geng, Hongwei Rasheed, Awais Xiao, Yonggui Cao, Shuanghe Fu, Luping Yan, Jun Wen, Weie Zhang, Yong Jing, Ruilian Xia, Xianchun He, Zhonghu PLoS One Research Article Water soluble carbohydrates (WSC) in stems play an important role in buffering grain yield in wheat against biotic and abiotic stresses; however, knowledge of genes controlling WSC is very limited. We conducted a genome-wide association study (GWAS) using a high-density 90K SNP array to better understand the genetic basis underlying WSC, and to explore marker-based breeding approaches. WSC was evaluated in an association panel comprising 166 Chinese bread wheat cultivars planted in four environments. Fifty two marker-trait associations (MTAs) distributed across 23 loci were identified for phenotypic best linear unbiased estimates (BLUEs), and 11 MTAs were identified in two or more environments. Liner regression showed a clear dependence of WSC BLUE scores on numbers of favorable (increasing WSC content) and unfavorable alleles (decreasing WSC), indicating that genotypes with higher numbers of favorable or lower numbers of unfavorable alleles had higher WSC content. In silico analysis of flanking sequences of trait-associated SNPs revealed eight candidate genes related to WSC content grouped into two categories based on the type of encoding proteins, namely, defense response proteins and proteins triggered by environmental stresses. The identified SNPs and candidate genes related to WSC provide opportunities for breeding higher WSC wheat cultivars. Public Library of Science 2016-11-01 /pmc/articles/PMC5089554/ /pubmed/27802269 http://dx.doi.org/10.1371/journal.pone.0164293 Text en © 2016 Dong et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Dong, Yan Liu, Jindong Zhang, Yan Geng, Hongwei Rasheed, Awais Xiao, Yonggui Cao, Shuanghe Fu, Luping Yan, Jun Wen, Weie Zhang, Yong Jing, Ruilian Xia, Xianchun He, Zhonghu Genome-Wide Association of Stem Water Soluble Carbohydrates in Bread Wheat |
title | Genome-Wide Association of Stem Water Soluble Carbohydrates in Bread Wheat |
title_full | Genome-Wide Association of Stem Water Soluble Carbohydrates in Bread Wheat |
title_fullStr | Genome-Wide Association of Stem Water Soluble Carbohydrates in Bread Wheat |
title_full_unstemmed | Genome-Wide Association of Stem Water Soluble Carbohydrates in Bread Wheat |
title_short | Genome-Wide Association of Stem Water Soluble Carbohydrates in Bread Wheat |
title_sort | genome-wide association of stem water soluble carbohydrates in bread wheat |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5089554/ https://www.ncbi.nlm.nih.gov/pubmed/27802269 http://dx.doi.org/10.1371/journal.pone.0164293 |
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