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Development of a Peptide ELISA for the Diagnosis of Aleutian Mink Disease

Aleutian disease (AD) is a common immunosuppressive disease in mink farms world-wide. Since the 1980s, counterimmunoelectrophoresis (CIEP) has been the main detection method for infection with the Aleutian Mink Disease Virus (AMDV). In this study, six peptides derived from the AMDV structural protei...

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Autores principales: Ma, Fanshu, Zhang, Lei, Wang, Yang, Lu, Rongguang, Hu, Bo, Lv, Shuang, Xue, Xianghong, Li, Xintong, Ling, Mingyu, Fan, Sining, Zhang, Hailing, Yan, Xijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5089682/
https://www.ncbi.nlm.nih.gov/pubmed/27802320
http://dx.doi.org/10.1371/journal.pone.0165793
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author Ma, Fanshu
Zhang, Lei
Wang, Yang
Lu, Rongguang
Hu, Bo
Lv, Shuang
Xue, Xianghong
Li, Xintong
Ling, Mingyu
Fan, Sining
Zhang, Hailing
Yan, Xijun
author_facet Ma, Fanshu
Zhang, Lei
Wang, Yang
Lu, Rongguang
Hu, Bo
Lv, Shuang
Xue, Xianghong
Li, Xintong
Ling, Mingyu
Fan, Sining
Zhang, Hailing
Yan, Xijun
author_sort Ma, Fanshu
collection PubMed
description Aleutian disease (AD) is a common immunosuppressive disease in mink farms world-wide. Since the 1980s, counterimmunoelectrophoresis (CIEP) has been the main detection method for infection with the Aleutian Mink Disease Virus (AMDV). In this study, six peptides derived from the AMDV structural protein VP2 were designed, synthesized, and used as ELISA antigens to detect anti-AMDV antibodies in the sera of infected minks. Serum samples were collected from 764 minks in farms from five different provinces, and analyzed by both CIEP (a gold standard) and peptide ELISA. A peptide designated P1 (415 aa–433 aa) exhibited good antigenicity. A novel ELISA was developed using ovalbumin-linked peptide P1 to detect anti-AMDV antibodies in mink sera. The sensitivity and specificity of the peptide ELISA was 98.0% and 97.5%, respectively. Moreover, the ELISA also detected 342 early-stage infected samples (negative by CIEP and positive by PCR), of which 43.6% (149/342) were true positives. These results showed that the peptide ELISA had better sensitivity compared with CIEP, and therefore could be preferable over CIEP for detecting anti-AMDV antibodies in serological screening.
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spelling pubmed-50896822016-11-15 Development of a Peptide ELISA for the Diagnosis of Aleutian Mink Disease Ma, Fanshu Zhang, Lei Wang, Yang Lu, Rongguang Hu, Bo Lv, Shuang Xue, Xianghong Li, Xintong Ling, Mingyu Fan, Sining Zhang, Hailing Yan, Xijun PLoS One Research Article Aleutian disease (AD) is a common immunosuppressive disease in mink farms world-wide. Since the 1980s, counterimmunoelectrophoresis (CIEP) has been the main detection method for infection with the Aleutian Mink Disease Virus (AMDV). In this study, six peptides derived from the AMDV structural protein VP2 were designed, synthesized, and used as ELISA antigens to detect anti-AMDV antibodies in the sera of infected minks. Serum samples were collected from 764 minks in farms from five different provinces, and analyzed by both CIEP (a gold standard) and peptide ELISA. A peptide designated P1 (415 aa–433 aa) exhibited good antigenicity. A novel ELISA was developed using ovalbumin-linked peptide P1 to detect anti-AMDV antibodies in mink sera. The sensitivity and specificity of the peptide ELISA was 98.0% and 97.5%, respectively. Moreover, the ELISA also detected 342 early-stage infected samples (negative by CIEP and positive by PCR), of which 43.6% (149/342) were true positives. These results showed that the peptide ELISA had better sensitivity compared with CIEP, and therefore could be preferable over CIEP for detecting anti-AMDV antibodies in serological screening. Public Library of Science 2016-11-01 /pmc/articles/PMC5089682/ /pubmed/27802320 http://dx.doi.org/10.1371/journal.pone.0165793 Text en © 2016 Ma et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ma, Fanshu
Zhang, Lei
Wang, Yang
Lu, Rongguang
Hu, Bo
Lv, Shuang
Xue, Xianghong
Li, Xintong
Ling, Mingyu
Fan, Sining
Zhang, Hailing
Yan, Xijun
Development of a Peptide ELISA for the Diagnosis of Aleutian Mink Disease
title Development of a Peptide ELISA for the Diagnosis of Aleutian Mink Disease
title_full Development of a Peptide ELISA for the Diagnosis of Aleutian Mink Disease
title_fullStr Development of a Peptide ELISA for the Diagnosis of Aleutian Mink Disease
title_full_unstemmed Development of a Peptide ELISA for the Diagnosis of Aleutian Mink Disease
title_short Development of a Peptide ELISA for the Diagnosis of Aleutian Mink Disease
title_sort development of a peptide elisa for the diagnosis of aleutian mink disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5089682/
https://www.ncbi.nlm.nih.gov/pubmed/27802320
http://dx.doi.org/10.1371/journal.pone.0165793
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