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A murine model of early Pseudomonas aeruginosa lung disease with transition to chronic infection
Pseudomonas aeruginosa (PA) remains an important pathogen in patients with cystic fibrosis (CF) lung disease as well as non-CF bronchiectasis and chronic obstructive airways disease. Initial infections are cleared but chronic infection with mucoid strains ensues in the majority of CF patients and sp...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5090221/ https://www.ncbi.nlm.nih.gov/pubmed/27804985 http://dx.doi.org/10.1038/srep35838 |
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author | Bayes, H. K. Ritchie, N. Irvine, S. Evans, T. J. |
author_facet | Bayes, H. K. Ritchie, N. Irvine, S. Evans, T. J. |
author_sort | Bayes, H. K. |
collection | PubMed |
description | Pseudomonas aeruginosa (PA) remains an important pathogen in patients with cystic fibrosis (CF) lung disease as well as non-CF bronchiectasis and chronic obstructive airways disease. Initial infections are cleared but chronic infection with mucoid strains ensues in the majority of CF patients and specific interventions to prevent this critical infection transition are lacking. The PA bead model has been widely used to study pulmonary P.aeruginosa infection but has limitations in animal husbandry and in accurately mimicking human disease. We have developed an adapted agar bead murine model using a clinical mucoid strain that demonstrates the key features of transition from transitory to chronic airways infection. Infected animals show very limited acute morbidity and mortality, but undergo infection-related weight loss and neutrophilic inflammation, development of anti-pseudomonal antibodies, variable bacterial clearance, endobronchial infection and microbial adaptation with PA small colony variants. We anticipate this model will allow research into the host and microbial factors governing this critical period in Pseudomonas aeruginosa pulmonary pathogenesis when transition to chronicity is occurring. |
format | Online Article Text |
id | pubmed-5090221 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50902212016-11-08 A murine model of early Pseudomonas aeruginosa lung disease with transition to chronic infection Bayes, H. K. Ritchie, N. Irvine, S. Evans, T. J. Sci Rep Article Pseudomonas aeruginosa (PA) remains an important pathogen in patients with cystic fibrosis (CF) lung disease as well as non-CF bronchiectasis and chronic obstructive airways disease. Initial infections are cleared but chronic infection with mucoid strains ensues in the majority of CF patients and specific interventions to prevent this critical infection transition are lacking. The PA bead model has been widely used to study pulmonary P.aeruginosa infection but has limitations in animal husbandry and in accurately mimicking human disease. We have developed an adapted agar bead murine model using a clinical mucoid strain that demonstrates the key features of transition from transitory to chronic airways infection. Infected animals show very limited acute morbidity and mortality, but undergo infection-related weight loss and neutrophilic inflammation, development of anti-pseudomonal antibodies, variable bacterial clearance, endobronchial infection and microbial adaptation with PA small colony variants. We anticipate this model will allow research into the host and microbial factors governing this critical period in Pseudomonas aeruginosa pulmonary pathogenesis when transition to chronicity is occurring. Nature Publishing Group 2016-11-02 /pmc/articles/PMC5090221/ /pubmed/27804985 http://dx.doi.org/10.1038/srep35838 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Bayes, H. K. Ritchie, N. Irvine, S. Evans, T. J. A murine model of early Pseudomonas aeruginosa lung disease with transition to chronic infection |
title | A murine model of early Pseudomonas aeruginosa lung disease with transition to chronic infection |
title_full | A murine model of early Pseudomonas aeruginosa lung disease with transition to chronic infection |
title_fullStr | A murine model of early Pseudomonas aeruginosa lung disease with transition to chronic infection |
title_full_unstemmed | A murine model of early Pseudomonas aeruginosa lung disease with transition to chronic infection |
title_short | A murine model of early Pseudomonas aeruginosa lung disease with transition to chronic infection |
title_sort | murine model of early pseudomonas aeruginosa lung disease with transition to chronic infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5090221/ https://www.ncbi.nlm.nih.gov/pubmed/27804985 http://dx.doi.org/10.1038/srep35838 |
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