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The influence of different nanostructured scaffolds on fibroblast growth

Skin serves as a protective barrier, modulating body temperature and waste discharge. It is therefore desirable to be able to repair any damage that occurs to the skin as soon as possible. In this study, we demonstrate a relatively easy and cost-effective method for the fabrication of nanostructured...

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Autores principales: Chung, I-Cheng, Li, Ching-Wen, Wang, Gou-Jen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5090312/
https://www.ncbi.nlm.nih.gov/pubmed/27877586
http://dx.doi.org/10.1088/1468-6996/14/4/044401
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author Chung, I-Cheng
Li, Ching-Wen
Wang, Gou-Jen
author_facet Chung, I-Cheng
Li, Ching-Wen
Wang, Gou-Jen
author_sort Chung, I-Cheng
collection PubMed
description Skin serves as a protective barrier, modulating body temperature and waste discharge. It is therefore desirable to be able to repair any damage that occurs to the skin as soon as possible. In this study, we demonstrate a relatively easy and cost-effective method for the fabrication of nanostructured scaffolds, to shorten the time taken for a wound to heal. Various scaffolds consisting of nanohemisphere arrays of poly(lactic-co-glycolic acid) (PLGA), polylactide and chitosan were fabricated by casting using a nickel (Ni) replica mold. The Ni replica mold is electroformed using the highly ordered nanohemisphere array of the barrier-layer surface of an anodic aluminum oxide membrane as the template. Mouse fibroblast cells (L929s) were cultured on the nanostructured polymer scaffolds to investigate the effect of these different nanohemisphere arrays on cell proliferation. The concentration of collagen type I on each scaffold was then measured through enzyme-linked immunosorbent assay to find the most effective scaffold for shortening the wound-healing process. The experimental data indicate that the proliferation of L929 is superior when a nanostructured PLGA scaffold with a feature size of 118 nm is utilized.
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spelling pubmed-50903122016-11-22 The influence of different nanostructured scaffolds on fibroblast growth Chung, I-Cheng Li, Ching-Wen Wang, Gou-Jen Sci Technol Adv Mater Focus Articles Skin serves as a protective barrier, modulating body temperature and waste discharge. It is therefore desirable to be able to repair any damage that occurs to the skin as soon as possible. In this study, we demonstrate a relatively easy and cost-effective method for the fabrication of nanostructured scaffolds, to shorten the time taken for a wound to heal. Various scaffolds consisting of nanohemisphere arrays of poly(lactic-co-glycolic acid) (PLGA), polylactide and chitosan were fabricated by casting using a nickel (Ni) replica mold. The Ni replica mold is electroformed using the highly ordered nanohemisphere array of the barrier-layer surface of an anodic aluminum oxide membrane as the template. Mouse fibroblast cells (L929s) were cultured on the nanostructured polymer scaffolds to investigate the effect of these different nanohemisphere arrays on cell proliferation. The concentration of collagen type I on each scaffold was then measured through enzyme-linked immunosorbent assay to find the most effective scaffold for shortening the wound-healing process. The experimental data indicate that the proliferation of L929 is superior when a nanostructured PLGA scaffold with a feature size of 118 nm is utilized. Taylor & Francis 2013-07-18 /pmc/articles/PMC5090312/ /pubmed/27877586 http://dx.doi.org/10.1088/1468-6996/14/4/044401 Text en © 2013 National Institute for Materials Science http://creativecommons.org/licenses/by-nc-sa/3.0/ Content from this work may be used under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 licence (http://creativecommons.org/licenses/by-nc-sa/3.0) . Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
spellingShingle Focus Articles
Chung, I-Cheng
Li, Ching-Wen
Wang, Gou-Jen
The influence of different nanostructured scaffolds on fibroblast growth
title The influence of different nanostructured scaffolds on fibroblast growth
title_full The influence of different nanostructured scaffolds on fibroblast growth
title_fullStr The influence of different nanostructured scaffolds on fibroblast growth
title_full_unstemmed The influence of different nanostructured scaffolds on fibroblast growth
title_short The influence of different nanostructured scaffolds on fibroblast growth
title_sort influence of different nanostructured scaffolds on fibroblast growth
topic Focus Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5090312/
https://www.ncbi.nlm.nih.gov/pubmed/27877586
http://dx.doi.org/10.1088/1468-6996/14/4/044401
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