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Amperometric catechol biosensor based on laccase immobilized on nitrogen-doped ordered mesoporous carbon (N-OMC)/PVA matrix

A functionalized nitrogen-containing ordered mesoporous carbon (N-OMC), which shows good electrical properties, was synthesized by the carbonization of polyaniline inside a SBA-15 mesoporous silica template. Based on this, through entrapping laccase onto the N-OMC/polyvinyl alcohol (PVA) film a faci...

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Autores principales: Guo, Meiqing, Wang, Hefeng, Huang, Di, Han, Zhijun, Li, Qiang, Wang, Xiaojun, Chen, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5090526/
https://www.ncbi.nlm.nih.gov/pubmed/27877681
http://dx.doi.org/10.1088/1468-6996/15/3/035005
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author Guo, Meiqing
Wang, Hefeng
Huang, Di
Han, Zhijun
Li, Qiang
Wang, Xiaojun
Chen, Jing
author_facet Guo, Meiqing
Wang, Hefeng
Huang, Di
Han, Zhijun
Li, Qiang
Wang, Xiaojun
Chen, Jing
author_sort Guo, Meiqing
collection PubMed
description A functionalized nitrogen-containing ordered mesoporous carbon (N-OMC), which shows good electrical properties, was synthesized by the carbonization of polyaniline inside a SBA-15 mesoporous silica template. Based on this, through entrapping laccase onto the N-OMC/polyvinyl alcohol (PVA) film a facilely fabricated amperometric biosensor was developed. Laccase from Trametes versicolor was assembled on a composite film of a N-OMC/PVA modified Au electrode and the electrochemical behavior was investigated. The results indicated that the N-OMC modified electrode exhibits electrical properties towards catechol. The optimum experimental conditions of a biosensor for the detection of catechol were studied in detail. Under the optimal conditions, the sensitivity of the biosensor was 0.29 A*M(−1) with a detection limit of 0.31 μM and a linear detection range from 0.39 μM to 8.98 μM for catechol. The calibration curve followed the Michaelis–Menten kinetics and the apparent Michaelis–Menten [Image: see text] was 6.28 μM. This work demonstrated that the N-OMC/PVA composite provides a suitable support for laccase immobilization and the construction of a biosensor.
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spelling pubmed-50905262016-11-22 Amperometric catechol biosensor based on laccase immobilized on nitrogen-doped ordered mesoporous carbon (N-OMC)/PVA matrix Guo, Meiqing Wang, Hefeng Huang, Di Han, Zhijun Li, Qiang Wang, Xiaojun Chen, Jing Sci Technol Adv Mater Papers A functionalized nitrogen-containing ordered mesoporous carbon (N-OMC), which shows good electrical properties, was synthesized by the carbonization of polyaniline inside a SBA-15 mesoporous silica template. Based on this, through entrapping laccase onto the N-OMC/polyvinyl alcohol (PVA) film a facilely fabricated amperometric biosensor was developed. Laccase from Trametes versicolor was assembled on a composite film of a N-OMC/PVA modified Au electrode and the electrochemical behavior was investigated. The results indicated that the N-OMC modified electrode exhibits electrical properties towards catechol. The optimum experimental conditions of a biosensor for the detection of catechol were studied in detail. Under the optimal conditions, the sensitivity of the biosensor was 0.29 A*M(−1) with a detection limit of 0.31 μM and a linear detection range from 0.39 μM to 8.98 μM for catechol. The calibration curve followed the Michaelis–Menten kinetics and the apparent Michaelis–Menten [Image: see text] was 6.28 μM. This work demonstrated that the N-OMC/PVA composite provides a suitable support for laccase immobilization and the construction of a biosensor. Taylor & Francis 2014-06-10 /pmc/articles/PMC5090526/ /pubmed/27877681 http://dx.doi.org/10.1088/1468-6996/15/3/035005 Text en © 2014 National Institute for Materials Science http://creativecommons.org/licenses/by/3.0/ Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence (http://creativecommons.org/licenses/by/3.0) . Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
spellingShingle Papers
Guo, Meiqing
Wang, Hefeng
Huang, Di
Han, Zhijun
Li, Qiang
Wang, Xiaojun
Chen, Jing
Amperometric catechol biosensor based on laccase immobilized on nitrogen-doped ordered mesoporous carbon (N-OMC)/PVA matrix
title Amperometric catechol biosensor based on laccase immobilized on nitrogen-doped ordered mesoporous carbon (N-OMC)/PVA matrix
title_full Amperometric catechol biosensor based on laccase immobilized on nitrogen-doped ordered mesoporous carbon (N-OMC)/PVA matrix
title_fullStr Amperometric catechol biosensor based on laccase immobilized on nitrogen-doped ordered mesoporous carbon (N-OMC)/PVA matrix
title_full_unstemmed Amperometric catechol biosensor based on laccase immobilized on nitrogen-doped ordered mesoporous carbon (N-OMC)/PVA matrix
title_short Amperometric catechol biosensor based on laccase immobilized on nitrogen-doped ordered mesoporous carbon (N-OMC)/PVA matrix
title_sort amperometric catechol biosensor based on laccase immobilized on nitrogen-doped ordered mesoporous carbon (n-omc)/pva matrix
topic Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5090526/
https://www.ncbi.nlm.nih.gov/pubmed/27877681
http://dx.doi.org/10.1088/1468-6996/15/3/035005
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