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Comparative assessment of antimicrobial efficacy of different hand sanitizers: An in vitro study

BACKGROUND: To evaluate the antimicrobial efficacy of four different hand sanitizers against Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecalis as well as to assess and compare the antimicrobial effectiveness among four different ha...

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Autores principales: Jain, Vardhaman Mulchand, Karibasappa, Gundabaktha Nagappa, Dodamani, Arun Suresh, Prashanth, Vishwakarma K., Mali, Gaurao Vasant
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5091001/
https://www.ncbi.nlm.nih.gov/pubmed/27857768
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author Jain, Vardhaman Mulchand
Karibasappa, Gundabaktha Nagappa
Dodamani, Arun Suresh
Prashanth, Vishwakarma K.
Mali, Gaurao Vasant
author_facet Jain, Vardhaman Mulchand
Karibasappa, Gundabaktha Nagappa
Dodamani, Arun Suresh
Prashanth, Vishwakarma K.
Mali, Gaurao Vasant
author_sort Jain, Vardhaman Mulchand
collection PubMed
description BACKGROUND: To evaluate the antimicrobial efficacy of four different hand sanitizers against Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecalis as well as to assess and compare the antimicrobial effectiveness among four different hand sanitizers. MATERIALS AND METHODS: The present study is an in vitro study to evaluate antimicrobial efficacy of Dettol, Lifebuoy, PureHands, and Sterillium hand sanitizers against clinical isolates of the aforementioned test organisms. The well variant of agar disk diffusion test using Mueller-Hinton agar was used for evaluating the antimicrobial efficacy of hand sanitizers. McFarland 0.5 turbidity standard was taken as reference to adjust the turbidity of bacterial suspensions. Fifty microliters of the hand sanitizer was introduced into each of the 4 wells while the 5(th) well incorporated with sterile water served as a control. This was done for all the test organisms and plates were incubated in an incubator for 24 h at 37΀C. After incubation, antimicrobial effectiveness was determined using digital caliper (mm) by measuring the zone of inhibition. RESULTS: The mean diameters of zones of inhibition (in mm) observed in Group A (Sterillium), Group B (PureHands), Group C (Lifebuoy), and Group D (Dettol) were 22 ± 6, 7.5 ± 0.5, 9.5 ± 1.5, and 8 ± 1, respectively. Maximum inhibition was found with Group A against all the tested organisms. Data were statistically analyzed using analysis of variance, followed by post hoc test for group-wise comparisons. The difference in the values of different sanitizers was statistically significant at P < 0.001. CONCLUSION: Sterillium was the most effective hand sanitizer to maintain the hand hygiene.
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spelling pubmed-50910012016-11-17 Comparative assessment of antimicrobial efficacy of different hand sanitizers: An in vitro study Jain, Vardhaman Mulchand Karibasappa, Gundabaktha Nagappa Dodamani, Arun Suresh Prashanth, Vishwakarma K. Mali, Gaurao Vasant Dent Res J (Isfahan) Original Article BACKGROUND: To evaluate the antimicrobial efficacy of four different hand sanitizers against Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecalis as well as to assess and compare the antimicrobial effectiveness among four different hand sanitizers. MATERIALS AND METHODS: The present study is an in vitro study to evaluate antimicrobial efficacy of Dettol, Lifebuoy, PureHands, and Sterillium hand sanitizers against clinical isolates of the aforementioned test organisms. The well variant of agar disk diffusion test using Mueller-Hinton agar was used for evaluating the antimicrobial efficacy of hand sanitizers. McFarland 0.5 turbidity standard was taken as reference to adjust the turbidity of bacterial suspensions. Fifty microliters of the hand sanitizer was introduced into each of the 4 wells while the 5(th) well incorporated with sterile water served as a control. This was done for all the test organisms and plates were incubated in an incubator for 24 h at 37΀C. After incubation, antimicrobial effectiveness was determined using digital caliper (mm) by measuring the zone of inhibition. RESULTS: The mean diameters of zones of inhibition (in mm) observed in Group A (Sterillium), Group B (PureHands), Group C (Lifebuoy), and Group D (Dettol) were 22 ± 6, 7.5 ± 0.5, 9.5 ± 1.5, and 8 ± 1, respectively. Maximum inhibition was found with Group A against all the tested organisms. Data were statistically analyzed using analysis of variance, followed by post hoc test for group-wise comparisons. The difference in the values of different sanitizers was statistically significant at P < 0.001. CONCLUSION: Sterillium was the most effective hand sanitizer to maintain the hand hygiene. Medknow Publications & Media Pvt Ltd 2016-09 /pmc/articles/PMC5091001/ /pubmed/27857768 Text en Copyright: © Dental Research Journal http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Jain, Vardhaman Mulchand
Karibasappa, Gundabaktha Nagappa
Dodamani, Arun Suresh
Prashanth, Vishwakarma K.
Mali, Gaurao Vasant
Comparative assessment of antimicrobial efficacy of different hand sanitizers: An in vitro study
title Comparative assessment of antimicrobial efficacy of different hand sanitizers: An in vitro study
title_full Comparative assessment of antimicrobial efficacy of different hand sanitizers: An in vitro study
title_fullStr Comparative assessment of antimicrobial efficacy of different hand sanitizers: An in vitro study
title_full_unstemmed Comparative assessment of antimicrobial efficacy of different hand sanitizers: An in vitro study
title_short Comparative assessment of antimicrobial efficacy of different hand sanitizers: An in vitro study
title_sort comparative assessment of antimicrobial efficacy of different hand sanitizers: an in vitro study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5091001/
https://www.ncbi.nlm.nih.gov/pubmed/27857768
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