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Legionella pneumophila prevents proliferation of its natural host Acanthamoeba castellanii
Legionella pneumophila is a ubiquitous, pathogenic, Gram-negative bacterium responsible for legionellosis. Like many other amoeba-resistant microorganisms, L. pneumophila resists host clearance and multiplies inside the cell. Through its Dot/Icm type IV secretion system, the bacterium injects more t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5091012/ https://www.ncbi.nlm.nih.gov/pubmed/27805070 http://dx.doi.org/10.1038/srep36448 |
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author | Mengue, Luce Régnacq, Matthieu Aucher, Willy Portier, Emilie Héchard, Yann Samba-Louaka, Ascel |
author_facet | Mengue, Luce Régnacq, Matthieu Aucher, Willy Portier, Emilie Héchard, Yann Samba-Louaka, Ascel |
author_sort | Mengue, Luce |
collection | PubMed |
description | Legionella pneumophila is a ubiquitous, pathogenic, Gram-negative bacterium responsible for legionellosis. Like many other amoeba-resistant microorganisms, L. pneumophila resists host clearance and multiplies inside the cell. Through its Dot/Icm type IV secretion system, the bacterium injects more than three hundred effectors that modulate host cell physiology in order to promote its own intracellular replication. Here we report that L. pneumophila prevents proliferation of its natural host Acanthamoeba castellanii. Infected amoebae could not undergo DNA replication and no cell division was observed. The Dot/Icm secretion system was necessary for L. pneumophila to prevent the eukaryotic proliferation. The absence of proliferation was associated with altered amoebal morphology and with a decrease of mRNA transcript levels of CDC2b, a putative regulator of the A. castellanii cell cycle. Complementation of CDC28-deficient Saccharomyces cerevisiae by the CDC2b cDNA was sufficient to restore proliferation of CDC28-deficient S. cerevisiae and suggests for the first time that CDC2b from A. castellanii could be functional and a bona fide cyclin-dependent kinase. Hence, our results reveal that L. pneumophila impairs proliferation of A. castellanii and this effect could involve the cell cycle protein CDC2b. |
format | Online Article Text |
id | pubmed-5091012 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50910122016-11-08 Legionella pneumophila prevents proliferation of its natural host Acanthamoeba castellanii Mengue, Luce Régnacq, Matthieu Aucher, Willy Portier, Emilie Héchard, Yann Samba-Louaka, Ascel Sci Rep Article Legionella pneumophila is a ubiquitous, pathogenic, Gram-negative bacterium responsible for legionellosis. Like many other amoeba-resistant microorganisms, L. pneumophila resists host clearance and multiplies inside the cell. Through its Dot/Icm type IV secretion system, the bacterium injects more than three hundred effectors that modulate host cell physiology in order to promote its own intracellular replication. Here we report that L. pneumophila prevents proliferation of its natural host Acanthamoeba castellanii. Infected amoebae could not undergo DNA replication and no cell division was observed. The Dot/Icm secretion system was necessary for L. pneumophila to prevent the eukaryotic proliferation. The absence of proliferation was associated with altered amoebal morphology and with a decrease of mRNA transcript levels of CDC2b, a putative regulator of the A. castellanii cell cycle. Complementation of CDC28-deficient Saccharomyces cerevisiae by the CDC2b cDNA was sufficient to restore proliferation of CDC28-deficient S. cerevisiae and suggests for the first time that CDC2b from A. castellanii could be functional and a bona fide cyclin-dependent kinase. Hence, our results reveal that L. pneumophila impairs proliferation of A. castellanii and this effect could involve the cell cycle protein CDC2b. Nature Publishing Group 2016-11-02 /pmc/articles/PMC5091012/ /pubmed/27805070 http://dx.doi.org/10.1038/srep36448 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Mengue, Luce Régnacq, Matthieu Aucher, Willy Portier, Emilie Héchard, Yann Samba-Louaka, Ascel Legionella pneumophila prevents proliferation of its natural host Acanthamoeba castellanii |
title | Legionella pneumophila prevents proliferation of its natural host Acanthamoeba castellanii |
title_full | Legionella pneumophila prevents proliferation of its natural host Acanthamoeba castellanii |
title_fullStr | Legionella pneumophila prevents proliferation of its natural host Acanthamoeba castellanii |
title_full_unstemmed | Legionella pneumophila prevents proliferation of its natural host Acanthamoeba castellanii |
title_short | Legionella pneumophila prevents proliferation of its natural host Acanthamoeba castellanii |
title_sort | legionella pneumophila prevents proliferation of its natural host acanthamoeba castellanii |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5091012/ https://www.ncbi.nlm.nih.gov/pubmed/27805070 http://dx.doi.org/10.1038/srep36448 |
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