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Interleukin-7 in Patients With Chronic Hepatitis B May Have Effect on T Follicular Helper Cells and Specific Cellular Immunity

BACKGROUND: In patients with chronic hepatitis B (CHB), the relation of interkeukin-7 (IL-7) to either the T follicular helper cells (Tfh cells) or to a specific cellular immune response is not clear. OBJECTIVES: The present study aims to explore the possible relationship of IL-7 to Tfh cells and to...

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Autores principales: Zhong, Hua, Xibing, Gu, Yaping, Dai, Zheng, Wang, Decai, Fu, Xiaoye, Guo, Hangyuan, Wu, Dong, Wang, Zhonghua, Lu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5091030/
https://www.ncbi.nlm.nih.gov/pubmed/27822258
http://dx.doi.org/10.5812/hepatmon.36068
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author Zhong, Hua
Xibing, Gu
Yaping, Dai
Zheng, Wang
Decai, Fu
Xiaoye, Guo
Hangyuan, Wu
Dong, Wang
Zhonghua, Lu
author_facet Zhong, Hua
Xibing, Gu
Yaping, Dai
Zheng, Wang
Decai, Fu
Xiaoye, Guo
Hangyuan, Wu
Dong, Wang
Zhonghua, Lu
author_sort Zhong, Hua
collection PubMed
description BACKGROUND: In patients with chronic hepatitis B (CHB), the relation of interkeukin-7 (IL-7) to either the T follicular helper cells (Tfh cells) or to a specific cellular immune response is not clear. OBJECTIVES: The present study aims to explore the possible relationship of IL-7 to Tfh cells and to hepatitis B virus (HBV)-specific cellular immune response in patients with CHB. METHODS: Ninety-one adult patients with CHB were divided into groups A, B, and C, according to the patients’ IL-7 levels (low, medium, and high). Tfh cells and HBV-specific cytotoxic T lymphocytes (CTLs) were detected with flow cytometry; IL-7 and IL-21 were determined with a double antibody sandwich enzyme-linked immunosorbent assay; and HBV DNA was determined by using a real-time fluorescent quantitative polymerase chain reaction. RESULTS: The results showed that the levels of IL-7, Tfh cells, IL-21, and HBV-specific CTLs of patients in group C were significantly higher than those of patients in group B, (P < 0.01 for each comparison) and that the levels of these four parameters of patients in group B were significantly higher than those of the patients in group A (P < 0.01 for each comparison). Meanwhile, the level of HBV DNA of the patients in group C was significantly lower than that of the patients in group B (P < 0.01), and that of the patients in group B was significantly lower than that of the patients in group A (P < 0.05). Multiple linear regression analyses showed that IL-7, Tfh cells, IL-21, and HBV-specific CTL might have effects on HBV DNA and that only the HBV-specific CTL had an independent effect on HBV DNA (P < 0.01). IL-7, Tfh cells, and IL-21 showed independent effects on HBV-specific CTL (P < 0.05, P < 0.01, and P < 0.01). CONCLUSIONS: This study suggests that the IL-7 level of CHB patients may be related to Tfh cells. In CHB patients, IL-7 possibly increases the level of Tfh cells and HBV-specific cellular immune responses and thereby reduces the HBV DNA level.
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spelling pubmed-50910302016-11-07 Interleukin-7 in Patients With Chronic Hepatitis B May Have Effect on T Follicular Helper Cells and Specific Cellular Immunity Zhong, Hua Xibing, Gu Yaping, Dai Zheng, Wang Decai, Fu Xiaoye, Guo Hangyuan, Wu Dong, Wang Zhonghua, Lu Hepat Mon Research Article BACKGROUND: In patients with chronic hepatitis B (CHB), the relation of interkeukin-7 (IL-7) to either the T follicular helper cells (Tfh cells) or to a specific cellular immune response is not clear. OBJECTIVES: The present study aims to explore the possible relationship of IL-7 to Tfh cells and to hepatitis B virus (HBV)-specific cellular immune response in patients with CHB. METHODS: Ninety-one adult patients with CHB were divided into groups A, B, and C, according to the patients’ IL-7 levels (low, medium, and high). Tfh cells and HBV-specific cytotoxic T lymphocytes (CTLs) were detected with flow cytometry; IL-7 and IL-21 were determined with a double antibody sandwich enzyme-linked immunosorbent assay; and HBV DNA was determined by using a real-time fluorescent quantitative polymerase chain reaction. RESULTS: The results showed that the levels of IL-7, Tfh cells, IL-21, and HBV-specific CTLs of patients in group C were significantly higher than those of patients in group B, (P < 0.01 for each comparison) and that the levels of these four parameters of patients in group B were significantly higher than those of the patients in group A (P < 0.01 for each comparison). Meanwhile, the level of HBV DNA of the patients in group C was significantly lower than that of the patients in group B (P < 0.01), and that of the patients in group B was significantly lower than that of the patients in group A (P < 0.05). Multiple linear regression analyses showed that IL-7, Tfh cells, IL-21, and HBV-specific CTL might have effects on HBV DNA and that only the HBV-specific CTL had an independent effect on HBV DNA (P < 0.01). IL-7, Tfh cells, and IL-21 showed independent effects on HBV-specific CTL (P < 0.05, P < 0.01, and P < 0.01). CONCLUSIONS: This study suggests that the IL-7 level of CHB patients may be related to Tfh cells. In CHB patients, IL-7 possibly increases the level of Tfh cells and HBV-specific cellular immune responses and thereby reduces the HBV DNA level. Kowsar 2016-08-15 /pmc/articles/PMC5091030/ /pubmed/27822258 http://dx.doi.org/10.5812/hepatmon.36068 Text en Copyright © 2016, Kowsar Corp http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Research Article
Zhong, Hua
Xibing, Gu
Yaping, Dai
Zheng, Wang
Decai, Fu
Xiaoye, Guo
Hangyuan, Wu
Dong, Wang
Zhonghua, Lu
Interleukin-7 in Patients With Chronic Hepatitis B May Have Effect on T Follicular Helper Cells and Specific Cellular Immunity
title Interleukin-7 in Patients With Chronic Hepatitis B May Have Effect on T Follicular Helper Cells and Specific Cellular Immunity
title_full Interleukin-7 in Patients With Chronic Hepatitis B May Have Effect on T Follicular Helper Cells and Specific Cellular Immunity
title_fullStr Interleukin-7 in Patients With Chronic Hepatitis B May Have Effect on T Follicular Helper Cells and Specific Cellular Immunity
title_full_unstemmed Interleukin-7 in Patients With Chronic Hepatitis B May Have Effect on T Follicular Helper Cells and Specific Cellular Immunity
title_short Interleukin-7 in Patients With Chronic Hepatitis B May Have Effect on T Follicular Helper Cells and Specific Cellular Immunity
title_sort interleukin-7 in patients with chronic hepatitis b may have effect on t follicular helper cells and specific cellular immunity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5091030/
https://www.ncbi.nlm.nih.gov/pubmed/27822258
http://dx.doi.org/10.5812/hepatmon.36068
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